The invention relates to the technical field of in-vitro diagnosis, and particularly relates to an
antithrombin III determination kit, and overcomes the defects that the determination rate of a determination kit in the prior art is low, and after determination is completed, a
reagent reaction is still carried out continuously, so that the numerical value of a determination experiment result is influenced, and a final result is deviated. The
antithrombin III determination kit comprises a
reagent I, a
reagent II, a speed-up solution and a stop solution, wherein the reagent I comprises
thrombin,
heparin, a
buffer solution and salt; the reagent II comprises a
chromogenic substrate of the
thrombin, a
buffer solution and
mannitol; and the speed-up solution comprises a disodium
hydrogen phosphatesolution; the stop solution comprises
polyvinyl chloride resin and
sodium azide. The reagent I, the reagent II, the speed-up solution and the stop solution are combined to form the kit, the
antithrombin III is determined quickly by utilizing the reagent I, the reagent II and the speed-up solution, and the reaction is stopped by utilizing the stop solution after determination is completed, so thatdata deviation caused by slow and
continuous reaction is avoided; the whole determination kit is convenient to use, quick in determination and accurate in data.