Cell counting method

Abstract

The method discloses a method of cell counting, which comprises the steps: the adherent culture of the cells of an experimental group to be counted and the cells of a criterion group with a known number is carried out on a plane surface; an original culture medium is soaked up, then the cells are washed, after that, glutaraldehyde solution is added for soaking, and the cells are dried in the air after washing by phosphate buffer solution; the fluorescence intensity of samples is detected by iraser; a standard curve is drawn by combing the known number of the cells and the fluorescence intensity so as to obtain a relation formula between the fluorescence intensity I standard and the number of the cells N standard: I standard equals to a multiplied by N standard and then plus b; and according to the standard curve and the fluorescence intensity of the cell samples in the experimental group, the number of the cells in the experimental group is calculated, namely, N experiment equals to (I experiment minus b) / a. The invention reflects the beneficial effects of simplifying experimental procedures, reducing cost, improving experimental precision and result parallelism.

Description

A method of counting cells technical field The present invention relates to a measuring method, in particular to the cell counting method and its application, especially the application of the method in intracellular western blotting. Background technique Cell counting is a frequently used technique in life science research. There are many methods for cell counting at present, but each has its own limitations: the classic hemocytometer method and its series of derivative methods (such as the cell counting method described in Chinese Patent Application No. Counting errors may occur due to factors such as the proficiency of the testing personnel, and the counting of a single sample takes a long time, and it is impossible to operate a large number of samples at the same time, which reduces the parallelism of the experimental results; although the staining method can meet the requirements of batch operations, each The consumption of dyes increases the cost of the experiment (...

AI Technical Summary

Problems solved by technology

In order to overcome the disadvantages of low counting accuracy and high cost in the prior art, the present invention provides a cell counting method with low cost, reliable data and good parallelism of batch processing results