43results about How to "High magnetic content" patented technology

The invention discloses a preparation method of a functional magnetic adsorbent for industrial wastewater treatment. The method is divided into four steps: (1) using a solvothermal method to prepare ferric oxide nanocrystal clusters with high magnetic saturation; (2) Using the sol-gel method to modify the surface of ferroferric oxide nanocrystal clusters into double bond functional groups; (3) using distillation precipitation polymerization to coat the surface of ferric oxide nanocrystal clusters with a polymer shell containing epoxy functional groups ; (4) The surface of the microspheres is modified into amino groups or carboxyl groups by the ring-opening reaction of epoxy groups. The magnetic adsorbent has high functional group content, fast magnetic response, and easy magnetic separation; regular structure, stable chemical and physical properties, and high dispersion stability in aqueous solution; its surface-modified amino and carboxyl functional groups can efficiently adsorb multiple A heavy metal or organic pollutant, which can be used in the field of industrial wastewater treatment. The method of the invention has simple operation, controllable process and good application prospect.

The invention relates to a preparing method used for utilizing protein to separate and purify nanometer magnetic composite microspheres. The preparing method comprises the following steps of: preparinThe invention relates to a preparing method used for utilizing protein to separate and purify nanometer magnetic composite microspheres. The preparing method comprises the following steps of: preparing magnetic nanoparticles, then adopting a silane coupling agent to modify the surfaces of the nanoparticles, mixing and dispersing the modified particles and high-molecular monomers, a cross linker ang magnetic nanoparticles, then adopting a silane coupling agent to modify the surfaces of the nanoparticles, mixing and dispersing the modified particles and high-molecular monomers, a cross linker and an initiator in water phase containing a surface active agent, carrying out emulsion polymerization and obtaining the magnetic composite microspheres. The prepared nanometer magnetic composite microd an initiator in water phase containing a surface active agent, carrying out emulsion polymerization and obtaining the magnetic composite microspheres. The prepared nanometer magnetic composite microspheres have particle diameters between 50 to 500 nanometers, rich surface function groups, good dispersion stability in medium, high efficiency of immune ligand coupling and strong magnetic responsivspheres have particle diameters between 50 to 500 nanometers, rich surface function groups, good dispersion stability in medium, high efficiency of immune ligand coupling and strong magnetic responsiveness, and are applicable to fast separation and purification of protein in biological samples.eness, and are applicable to fast separation and purification of protein in biological samples.

The invention discloses a method for preparing nuclear shell type high magnetic content super paramagnetic microsphere. Magnetic powder is treated at certain temperature with nitric acid and urea to obtain magnetic fluid; a certain amount of formaldehyde is added to solidify the magnetic fluid under the condition of water bath after the magnetic fluid is aged for days, and thus, magnetic microspheres are obtained; a proper amount of dispersant, acrylamide and crylic acid are added to the suspension of the magnetic microsphere to prepare the mixed liquid, and then the mixed liquid stands for one day in low temperature; then a certain amount of absolute ethyl alcohol and diisopropyl azodicarboxylate are added to the mixed liquid to carry out water bath heating and deoxidation processes; thetemperature is regulated to initiate reaction after PH value is regulated to neutrality; a product is washed after the reaction, and thus, the final product-magnetic microsphere with acrylamide-acrylic copolymer shell is obtained. The obtained product-nuclear shell type magnetic microsphere of the invention has the characteristics of high magnetic content, uniform grain diameter, few production process steps and low price of raw material and can be cross-linked with antibodies to prepare various types of immune diagnostic reagents.

The invention belongs to the technical field of modification of nano-starch microspheres, and in particular relates to a method for coating nano-starch microspheres by atomic layer deposition. In the present invention, nano-starch microspheres are placed in an ultrasonic vertical fluidized atomic layer deposition device, and ultrasonic vibration is turned on. The surface of the ball forms a single layer of chemical adsorption through the exchange of active functional groups and completes a self-limiting chemical half-reaction to form a dense film, which covers all parts of the surface with a film of uniform thickness. The coating uniformity of the nano-film produced by the atomic layer deposition technology in the present invention is high, especially for the nano-starch microspheres with small particles, which can be uniformly coated, and the formed nano-film has a dense structure, uniform thickness, and excellent consistency Due to the characteristics of its reaction mechanism, it can realize the coating of nano-starch microspheres with different particle sizes.

The invention specifically relates to surface carboxyl-functionalized core-shell type magnetic composite microballoons and a preparation method thereof, belonging to the technical field of functional materials. According to the invention, the core of the core-shell type magnetic composite microballoons is a magnetic ferriferrous oxide nanoparticle cluster, and the shell of the microballoons is a cross-linked carboxyl-containing polymer network. The preparation method comprises the following steps: at first, preparing the citric acid stabilized magnetic nanoparticle cluster (thereafter referred as magnetic cluster for short); then, modifying the surface of the magnetic cluster with active vinyl function groups by using the sol-gel method; finally, preparing the monodisperse core-shell type magnetic polymer composite microballoons which have high magnetic responsibility and contain abundant active carboxyl groups on their surface through distillation-precipitation polymerization. A variety of bioactive molecules can be bonded with the surface of the core-shell type microballoons through chemical modification for application in the field of biomedicine. Furthermore, the method can accurately control the thickness and cross-linking degree of the shell of polymers. The invention has the advantages of simple operation and controllable process and has a good application prospect.

The invention relates to a preparation method and application of a core-shell magnetic composite microsphere that can be used for separating glycoproteins. The core of the core-shell magnetic composite microsphere of the present invention is a cluster of magnetic ferric iron tetroxide nanoparticles, and the shell is a cross-linked polymer network rich in carboxyl groups, and then the carboxyl group and aminophenyl boron ester amidation reaction for surface modification, through A large number of boron esters immobilized on the surface can rapidly separate and enrich glycoproteins under physiological conditions. Firstly, sodium citrate-stabilized magnetic nanoparticle clusters were prepared, followed by the sol-gel method to modify the surface of the magnetic clusters with active vinyl functional groups, and then a highly magnetically responsive monodisperse surface rich in carboxyl groups was prepared by reflux precipitation polymerization The core-shell magnetic polymer composite microspheres were modified with aminophenylboronate and carboxyl groups for amidation reaction to modify a large number of boronate groups, and finally the separation and enrichment of glycoproteins was carried out. The method of the invention is simple, the process is controllable, the efficiency of separating and purifying glycoprotein is high, and it can be separated and enriched under physiological conditions.

The invention discloses a method for preparing a magnetic liposome. The medicine is coated inside the magnetic liposome; and magnetic particles are coated outside the liposome. The preparation method comprises the following steps that: a yolk lecithin solution and a cholesterin solution are mixed in an eggplant-shaped bottle according to the molar ratio of 2:1 to 2.5:1; the mixture is subjected to rotary evaporation to form a liposome film; then a phosphate buffering solution is used to carry out full hydration on the liposome film to obtain the liposome of which the surfaceis provided with negative charges; then, cationic polyelectrolyte-poly diallyl dimethyl ammonium chloride, anionic polyelectrolyte which is sodium polystyrene sulfonate and cationic polyelectrolyte which is poly diallyl dimethyl ammonium chloride are subjected to alternate adsorption; and finally, through the charge action and a self-assembling method, Fe3O4 magnetic particles of which the surfaces are provided with negative charges are deposited on the surface of the liposome to obtain the magnetic liposome. As the inside of the liposome is packed with the medicine, the magnetic particles are deposited on the surface of the liposome and a finite space of the inner space of the liposome is totally packed with the medicine, the packing amount of the medicine is improved and the magnetic content is simultaneously improved.

The invention discloses a preparation method of a nuclear-shell type magnetic composite microsphere which can be used for separating histidine protein, and an application of the nuclear-shell type magnetic composite microsphere. The core of the nuclear-shell type magnetic composite microsphere is a magnetic ferriferrous oxide nano particle cluster, the shell of the nuclear-shell type magnetic composite microsphere is a crosslinked polymer network rich in imidazole groups, the imidazole groups are chelated with the nickel ions to ensure that a great number of the nickel ions are fixed on the surface of the microsphere, natural histidine protein or recombinant protein which contains six histidine tags is rapidly separated through the interaction of the fixed nickel ions and the imidazole groups in histidine under neutral conditions. The preparation method comprises the steps of firstly preparing a sodium citrate stabilized magnetic nano particle cluster; modifying active vinyl functional groups on the surface of the magnetic cluster by adopting a sol-gel method; then preparing a nuclear-shell type magnetic polymer composite microsphere with the surface rich in imidazole groups, which has high magnetic responsibility and monodispersity, through backflow, precipitation and polymerization; then performing complexing on the nickel ions lack in electrons by using imidazole rich in the electrons; finally enriching or removing histidine protein. The preparation method disclosed by the invention is simple, controllable in process and high in efficiency of separating and purifying histidine protein, can be recycled for many times, and has good application prospects.