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Monodisperse strong-magnetism immunologic nanoparticles and preparation method thereof

A nano-microsphere and magnetic nanotechnology, applied in the direction of magnetic materials, magnetic objects, inorganic materials, etc., can solve the problems of reduced magnetic content of magnetic nanoparticles, reduced practical application effect, difficulty in achieving monodispersion, etc., and achieve particle size distribution Narrow, easy to industrialized mass production, good suspension and stability

Active Publication Date: 2016-08-17
杭州卓立纳米科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the prior art, the methods used to prepare magnetic nano-microspheres mainly include embedding method, polymerization method, etc. The main principle is to wrap magnetic nanoparticles in polymer microspheres. Since the surface of magnetic particles needs to be wrapped with enough polymers, It leads to a small amount of magnetic nanoparticles, which leads to a decrease in the magnetic content of the entire magnetic nanoparticles, an increase in the non-magnetic content, and it is often difficult to achieve monodispersion when they are agglomerated together
Such magnetic nano-microspheres have the following disadvantages: first, due to their poor dispersion and easy agglomeration, their specific surface area becomes smaller, which greatly reduces the practical application effect; second, due to their small magnetic content and weak magnetism, they are usually used in It takes dozens of seconds or even longer for enrichment, which greatly prolongs the operation time in the application. At the same time, in order to avoid this problem too much, it is necessary to increase the volume of magnetic beads or connect multiple magnetic beads together. The steric hindrance becomes larger, the sensitivity decreases, and the suspension becomes worse; third, the magnetic nanospheres of the prior art often cannot meet the practical application in terms of regularity in shape, uniformity of particle size, and content of surface functional active groups. needs

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  • Monodisperse strong-magnetism immunologic nanoparticles and preparation method thereof
  • Monodisperse strong-magnetism immunologic nanoparticles and preparation method thereof

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preparation example Construction

[0051] A method for preparing monodisperse strong magnetic immune nano-microspheres of the present invention comprises the following steps:

[0052] Step 1, preparation of iron ferric oxide nanoparticles;

[0053]Step 2, preparation of monodisperse silanol magnetic nano-microspheres;

[0054] Step 3, preparation of monodisperse aminated magnetic nanospheres;

[0055] Step 4, preparation of monodisperse strong magnetic immune nano-microspheres.

[0056] Specifically, each step includes the following in detail:

[0057] Step one includes the following steps:

[0058] S1. Take Fe with a concentration of 0.1-3mol / L 3+ salt solution and Fe 2+ 1:1 to 10:1 by volume, take 10-100mL of the mixed solution, add it to 5-500mL aqueous solution containing 0.1%-10% emulsifier, and mix well to obtain the mixed solution 1;

[0059] S2. Add 20%-80% polyethylene glycol-6000 aqueous solution 20-100mL to the mixed solution 1 to obtain the mixed solution 2;

[0060] S3. Heat the mixed soluti...

Embodiment 1

[0081] Include the following steps:

[0082] S1. Accurately weigh 8.94g of ferric sulfate and 4.34g of ferrous sulfate, dissolve them in 200mL of aqueous solution with 0.25mL of emulsifier added, and mix well to obtain mixed solution 1;

[0083] S2. Add 100 mL of 25% polyethylene glycol 6000 aqueous solution to the mixed solution 1 to obtain the mixed solution 2;

[0084] S3. Heat the mixed solution 2 to 20° C., and slowly add 0.1 ml of ammonia diluent with a concentration of 20% dropwise at the same time, keep the temperature and continue to react for 1 hour to obtain the particle 1;

[0085] S4. Wash the particles 1 three times with absolute ethanol and ultrapure water respectively, and perform magnetic separation on the particles 1, and dry them at a temperature of 65° C. for 2 hours to obtain the ferric oxide nanoparticles;

[0086] S5. Disperse the ferric ferric oxide nanoparticles in 3% sodium dodecylbenzenesulfonate aqueous solution, then add 100 mL of a mixed solution...

Embodiment 2

[0098] S1, take Fe with a mass ratio of 3 3+ salt solution and Fe 2+ salt solution, mixed uniformly at a volume ratio of 1:1, took 10 milliliters and added it to an aqueous solution containing 0.1% emulsifier, and mixed thoroughly to obtain a mixed solution 1;

[0099] S2. Add 20 ml of 20% polyethylene glycol 6000 aqueous solution to the mixed solution 1 to obtain the mixed solution 2;

[0100] S3. Heat the mixed solution 2 to 50° C., and slowly add 0.5 ml of ammonia diluent with a concentration of 75% dropwise at the same time, keep the temperature and continue to react for 3 hours to obtain the particle 1;

[0101] S4. Wash the particles 1 three times with absolute ethanol and ultrapure water respectively, and perform magnetic separation on the particles 1, and dry them at a temperature of 65° C. for 2 hours to obtain the ferric oxide nanoparticles;

[0102] S5. Disperse the ferric ferric oxide nanoparticles in an aqueous solution containing 1% sodium dodecylbenzenesulfona...

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Abstract

The invention discloses monodisperse strong-magnetism immunologic nanoparticles. The particle size of the monodisperse strong-magnetism immunologic nanoparticles ranges from 5 nm to 5 microns. The invention further discloses a preparation method of the monodisperse strong-magnetism immunologic nanoparticles. The method includes the following steps that firstly, ferroferric oxide nanoparticles are prepared; secondly, monodisperse silicon hydroxyl magnetic nanoparticles are prepared; thirdly, monodisperse amination magnetic nanoparticles are prepared; fourthly, the monodisperse strong-magnetism immunologic nanoparticles are prepared. The monodisperse strong-magnetism immunologic nanoparticles and the preparation method thereof have the advantages that superparamagnetism is good, the magnetic content is high, dispersity is good, the agglomeration phenomenon dose not exist, the appearance is regular and standard, the particle size is unified and controllable, and functional modification is easy.

Description

technical field [0001] The invention relates to the technical field of magnetic nanometer materials, in particular to a monodisperse strong magnetic immune nanometer microsphere and a preparation method thereof. Background technique [0002] With the development of nanotechnology, nanomaterials are more and more widely used in the field of biomedicine, especially magnetic nanoparticles composed of magnetic microspheres and nanomaterials, because of their magnetic responsiveness, superparamagnetism and good biological properties. Compatibility and other advantages have good application prospects in the fields of biotechnology and biomedicine. [0003] In the prior art, the methods used to prepare magnetic nano-microspheres mainly include embedding method, polymerization method, etc. The main principle is to wrap magnetic nanoparticles in polymer microspheres. Since the surface of magnetic particles needs to be wrapped with enough polymers, As a result, the amount of magnetic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H01F1/01H01F41/00
CPCH01F1/0054H01F1/01
Inventor 杨磊
Owner 杭州卓立纳米科技有限公司
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