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Immune magnetic microsphere and preparing process and usage thereof

A technology of magnetic microspheres and microspheres, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low content of surface functional groups, complex preparation process, high equipment requirements, etc., to achieve rich functional groups and simple preparation process , the effect of high coupling efficiency

Inactive Publication Date: 2005-09-14
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the defects of existing immunomagnetic microspheres such as a small amount of residual magnetism, less surface functional group content, complex preparation process, high equipment requirements and low yield, so as to provide a Immunomagnetic microspheres with no remanence, rich surface functional groups, high immunoligand coupling efficiency, simple preparation process and high yield, and preparation method and use thereof

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, preparation immunomagnetic microsphere I of the present invention

[0039] Preparation of oleic acid-coated Fe using a conventional chemical co-precipitation method 3 o 4 Magnetic fluid: Add 0.086mol ferrous chloride (FeCl 2 4H 2 O) and 0.173mol ferric chloride (FeCl 3 ·6H 2 O), in nitrogen N 2 Under protection, the temperature was raised to 90°C, and the solution containing 0.956mol NH 3 ·H 2 O aqueous solution, and about 15ml of oleic acid was added dropwise, and the temperature was continued for 30min. After being separated by a magnet, it was repeatedly washed with deionized water to obtain black lumps of oleic acid-coated Fe 3 o 4 ferrofluid. The total weight of the magnetic fluid is about 30g, which contains Fe 3 o 4 About 10g, observed under transmission electron microscope, Fe 3 o 4 The particles are nearly spherical, with superparamagnetic properties, and the average particle size is 8nm.

[0040] Preparation of magnetic polymer micr...

Embodiment 2

[0042] Embodiment 2, preparation immunomagnetic microsphere II of the present invention

[0043] Preparation of oleic acid-coated Fe using a conventional chemical co-precipitation method 3 o 4 Magnetic fluid: add 0.17mol ferrous chloride (FeCl 2 4H 2 O) and 0.34mol ferric chloride (FeCl 3 ·6H 2 O), in nitrogen N 2 Under protection, heat up to 70°C, pour 56ml of 25% NaHCO 3 Aqueous solution, and about 20ml of oleic acid was added dropwise, and the constant temperature was continued until the upper layer was clear. After being separated by a magnet, it was washed repeatedly with deionized water to obtain black lumps of oleic acid-coated Fe 3 o 4 ferrofluid. The total weight of the magnetic fluid is about 60g, which contains Fe 3 o 4 About 20g, observed under transmission electron microscope, Fe 3 o 4 The particles are nearly spherical, with superparamagnetic properties, and the average particle size is 12nm.

[0044] Preparation of magnetic polymer microspheres by e...

Embodiment 3

[0046] Embodiment 3, preparation immunomagnetic microsphere III of the present invention

[0047] Preparation of oleic acid-coated Fe using a conventional chemical co-precipitation method 3 o 4 Magnetic fluid: add 0.20mol ferrous chloride (FeCl 2 4H 2 O) and 0.40mol ferric chloride (FeCl 3 ·6H 2 O), in nitrogen N 2 Under protection, heat up to 80°C, pour 65ml of ammonia-containing aqueous solution, and drop about 35ml of oleic acid, continue to keep constant temperature until the upper layer is clear, separate with a magnet, and wash repeatedly with deionized water to obtain black lumps of oleic acid. Covered Fe 3 o 4 ferrofluid. The total weight of the magnetic fluid is about 75g, which contains Fe 3 o 4 About 24g, observed under transmission electron microscope, Fe 3 o 4 The particles are nearly spherical, with superparamagnetic properties, and the average particle size is 18nm.

[0048] Preparation of magnetic polymer microspheres by emulsion-suspension polymer...

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Abstract

This invention relates to an immunological magnetic microballoon, the process method of which comprises following steps: fully mixing oleic acid coating Fe3O4 magnetofluid and polymer monomer, cross linker and initiating agent to form oil phase; floating them in polyvinyl alcohol aqueous phase; polymerizing emulsion and suspension to process magnetic polymer microballoon; then introducing functional group on microballon surface through aminolysis reaction.The magnetic microballon is able to couple special immune genin on its surface, then these special immune genin further identify corresponding antibody, antigen and bioepiderm or directly use amidogen on immune magnetic microballon surface to identify antibody, antigen and bioepiderm able to react with amidogen.

Description

technical field [0001] The invention relates to an immunomagnetic microsphere, its preparation method and application. Background technique [0002] Immunomagnetic microsphere technology is a new technology developed by combining immunology and magnetic carrier technology. It is a new type of material that emerged in the 1970s. Immunomagnetic microspheres are usually composed of inorganic magnetic carriers and polymer shells, and are chemically coupled to specific immunoligands such as proteins, antibodies, antigens, and lectins on their surfaces. These immune ligands can further recognize corresponding antibodies, antigens, biotin, etc. in the reaction medium to achieve the purpose of separation or detection. The magnetic carrier has superparamagnetism, that is, it can generate magnetism in an external magnetic field, and there is no residual magnetism when the external magnetic field disappears. Immunomagnetic microspheres have the characteristics of superparamagnetism ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/547
Inventor 刘先桥官月平马志亚刘会洲杨宇吴小兵
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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