The invention relates to a multi-functional air microorganism sampling case, comprising two cases of I case and II case. The I case comprises a multi-functional air microorganism sampler, a second graded solid bumping sampler, a silicone tube, a charger and a multi-functional air microorganism sampler bracket. The II case comprises a plurality of all-glass liquid bumping samplers, a water filtering sampler, a liquid bumping sampler bracket, a plurality bottles of disinfection solution, a plurality of flat utensils, a plurality of plastic centrifuge tubes and auxiliary tools. The multi-functional air microorganism sampling case solidifies the three modes of the second graded bumping sampling, the liquid bumping sampling and water filtering sampling, which can collect the bacteria, fungi and viruses in the air, and also can collect and condense the microorganism in the polluted water. The multi-functional air microorganism sampling case has the characteristics of simple operation and portable carrying, which can be directly used for rapid inspecting on site, and also can be widely used in environmental protection, medical health, food industry, fermentation industry, medical preparation industry, agriculture and stockbreeding and the related research departments.