The invention relates to a method for separating and purifying
plant ice
structural protein, which belongs to the field of
food additive separation. The method uses cold-stress plants as raw materials to separate and purify the ice
structural protein by leaching and adopting a
cloud point extraction method, and comprises the following steps: firstly, adopting
buffer solution containing a non-ionic surfactant to leach out the ice
structural protein from the
plant raw materials; secondly, performing
cloud point extraction by adopting a micellar solution formed by the non-ionic surfactant, wherein the mixture is divided into a water phase and a micellar phase after being heated to the
cloud point temperature, hydrophobic impurities are enriched at the micellar phase, while the hydrophilic ice structural
protein is kept at the water phase; and finally, performing
dialysis concentration and
freeze drying to obtain a white ice structural
protein mixture. A
differential scanning calorimetry is adopted to perform
thermal hysteresis activity detection on a separated and purified sample to prove that the product has high
thermal hysteresis activity. The method has the advantages of simple
separation process, low production cost, no volatile
organic solvent in the process of extraction and high purification efficiency, and is advantageous for producing the ice structural
protein industrially and making full use of
plant resources.