The invention relates to a manufacturing method and application of a non-reproducible perovskite fluorescent anti-counterfeiting label; the manufacturing method comprises the following steps: preparing a substrate with a patterned lyophilic and lyophobic array, preparing a precursor droplet array substrate, and heating the substrate to obtain the perovskite fluorescent anti-counterfeiting label. According to the invention, different patterns are obtained by combining the solid patterns and the hollow patterns, and unique pattern morphology textures are obtained by adjusting the temperature. According to the invention, the solid pattern and the hollow pattern can be set, and the solid pattern and the hollow pattern can also be combined, so that anti-counterfeiting patterns set by different shape classification symbols are obtained, and the potential of the anti-counterfeiting label in commercial anti-counterfeiting application is improved. By using the method disclosed by the invention, the crystal films with highly similar shapes can be prepared, but each crystal film has irreproducible textures, and the reflection of the textures in a fluorescence image is unique light and shade distribution. The anti-counterfeit label is low in cost, the verification process is simple and efficient, and the anti-counterfeit label has a good market prospect.