121 results about "Protein digestion" patented technology

Disclosed are an apparatus, system, and method for performing electrospray of biomolecules, particularly peptides, polypeptides, and proteins. The apparatus comprises at least (1) a microfluidic substrate for containing an electrospray microchannel for delivering analyte molecules to a side edge of the substrate, and (2) a porous membrane attached to the side edge for performing electrospray from the exposed membrane surface. In one preferred embodiment, the exposed membrane surface is positioned above a target surface for depositing analyte molecules onto the target surface by electrospray. In another preferred embodiment, a proteolytic enzyme is bound to the porous membrane for performing protein digestion during electrospray.

The present invention relates to a nutritional supplement composition, comprising a therapeutically effective amounts of Vitamin C, Vitamin D3, Thiamin, Riboflavin, Niacin, Vitamin B6, Folic acid, Vitamin B12, Pantothenic acid, Calcium, Magnesium, Zinc, Chromium, Sugar, Protein, Acetyl-L-Carnitine, Dimethylaminoethanol complex, Phosphatidylserine complex, L-Glutamine, N-Acetyl-L-Tyrosine, L-Phenylalanine, Taurine, Methionine, Valine, Isoleucine, 5 Hydroxytryptophan, L-Taurine, N-Acetyl-Tyrosine, N-Acetyl-L-Cysteine, Alpha Lipoic Acid, Alpha Glycerylphosphoricholine complex, Bacopa Monnieri extract, Gingko Biloba extract, Passion flower, Lemon Balm, Gotu Kola, Ashwagandha, Choline Bitartrate complex, Panax Ginseng extract, Turmeric, Organic freeze dried fruit juice blends (concord grape, red raspberry, pineapple, cranberry, acai, pomegranate, acerola cherry, bilberry, lingonberry, black currant, aronia, sour cherry, black raspberry), Organic freeze dried greens blends (barley grass, broccoli, beet, carrot, alfalfa, oat), and Protein digestive enzyme blends (Protease 4.5, peptidase, bromelain, protease 6.0, protease 3.0, L planatrum, B bifidum) in a mixture to provide optimal cognitive function.

The invention relates to a liquid forage and the preparation method, which comprises the following components in weight percentage: corn 20 to 60 percent, puffing soybean 15 to 50 percent, whey powder 2 to 15 percent, blood plasma protein 1 to 10 percent, fish meal 2 to 15 percent, treacle 1 to 7 percent, soybean oil 1 to 5 percent , CaHPO4 0.1 to 2 percent, stone powder 0.1 to 1 percent, NaCl 0.1 to 2 percent, choline 0.1 to 1 percent, L-Lys (L- lysine salt) 0.1 to 1 percent, DL-Met (methionine) 0.1 to 1 percent, pre-mixture 1.00 to 4.00 percent, and deflocculant 0.1 to 2 percent. Compared with the dry forage, the invention enhances the food intake of weaned newborn pigs; the invention also increases the feeding speed; the liquid forage can avoid the damages of dry forage to the intestinal wall morphology of weaned newborn pigs; liquid forage is beneficial to the inner environment of the digestive canal of newborn pigs; the enhancement of growing performance of early weaning newborn pigs can also be gained from the increase of ADFI, and simultaneously low pH value is also helpful to the protein digestion in the stomach of newborn pigs. The invention has the advantages of the regulation of the growing performance, the influence on the immunity function, and the balance of intestinal microbiological flora of early isolated weaning newborn pigs to reduce the cultivation cost greatly.

The present invention provides compositions for the therapeutic and / or cosmetic treatment of Elastin comprising tissues. Therapeutic and cosmetic compositions comprising an elastin digest stimulate the endogenous production of Elastin and appear to enhance the elasticity of the skin and provide an external supply of peptide precursors of Elastin that penetrate into the tissue to which it is applied. The present invention describes compositions containing an elastin digest derived from proteolytic digestion of insoluble elastin derived from mammalian ligaments with a protein digesting composition, such as proteinase K. The elastin digest is a mixture of elastin peptides wherein the elastin peptide mixture comprises peptides of the sequence GXXPG, wherein X represents one of the natural amino acids. The elastin digest of the present invention may also comprise epitopes of cytokines, growth factors and di-peptides. Methods of using these elastin digest comprising compositions for treating tissues in need of increased elasticity and or Elastin are described.

The invention discloses a protein digestion stability analyzing method based on mass spectrometric detection and application thereof. The method includes the following steps of preparing a sample protein solution and a contrast protein solution to simulate stomach / intestine fluid, conducting stomach / intestine fluid digestion tests through a prepared sample and the contrast protein solution according to the reaction time of 0 second, 15 second, 2 minutes, 15 minutes, 30 minutes and 60 minutes, meanwhile setting a stomach / pancreas protease contrast and a sample protein contrast without stomach / pancreas protease, conducting SDS-PAGE electrophoresis on the obtained sample, conducting in-gel digestion on electrophoresis bands at different time moments of 0 second, 15 second, 2 minutes, 15 minutes, 30 minutes and 60 minutes, conducting liquid chromatography-mass spectrometry / mass spectrum (LC-MS / MS) detection, and retrieving data through Mascot software to obtain an identification map number of sample protein matched peptide fragments. The invention further discloses the application of the method in judging the digestion stability of foreign protein in transgenic rice. The method is free of antibody and high in targeted performance, sensitivity and accuracy and can be accurately used for judging the digestion stability of food and other foreign protein.

The present invention discloses a feather protein peptide feed additive and an application thereof. The preparation method for the feather protein peptide feed additive comprises: 1, mixing a substrate and water to obtain a solid state fermentation culture medium A, inoculating saccharomyces cerevisiae and saccharomycopsis fibuligera in the solid state fermentation culture medium A, culturing for 28-30 hours at a temperature of 30 DEG C, and drying the resulting culture at a temperature of 35-45 DEG C after culturing to obtain a yeast culture; and 2, mixing avian feather powder and the yeast culture, adding water to the resulting mixed material, uniformly stirring to obtain a solid state fermentation culture medium B, respectively inoculating bnfillus licheniformis and bacillus subtilis in the solid state fermentation culture medium B, and carrying out fermentation culture for 30-45 hours at a temperature of 35-38 DEG C to obtain the feather protein peptide feed additive. The feather protein peptide feed additive of the present invention has the following advantages that: feather protein digestion absorption efficiency can be 90%; the additive contains a variety of bioactive peptides, and provides digestion and growth promotion effects; the additive has natural fermentation flavor and good palatability; and pica of livestock and poultry can be significantly improved.

The invention provides a bighead feed with low cost. A high dosage of fermented cottonseed meal and a high dosage of fermented rapeseed meal are added and used as main raw materials; furthermore, the use of soybean meal and the use of animal protein raw materials are eliminated; and the high-protein-content bighead feed is manufactured by adding corn alcohol grain, wheat, monocalcium phosphate and various vitamins and trace elements. Toxic and harmful substances in the fermented cottonseed meal and the fermented rapeseed meal are reduced, and the fermented cottonseed meal and the fermented rapeseed meal are rich in small peptide, lactic acid, acetic acid and glutamic acid; various probiotics, vitamins, enzymic preparations and the like are retained and are favorable for improvement of protein digestion and absorption utilization rate; the pollution of residual feeds to a water body is reduced; the normal intestinal microecology of bigheads is retained, and the intestinal health of the bighead is balanced; and the feed coefficient is reduced.

The invention discloses a large-scale preparation method of a recombinant staphylococcus aureus vaccine. The method comprises the following steps: opening a protein working seed lot, inoculating the protein working seed lot strain in a conical flask, inoculating first-generation production strain in a seeding tank, performing continuous second-generation cultivation, inoculating the second-generation production strain in a fermentation tank, and performing continuous third-generation cultivation; centrifugally removing supernate from a bacterial solution through a continuous flow after fermentation is completed; and performing bacterium body dissolving and bacterium disruption; performing combination, washing the mixture after combination is completed, performing digestion, purifying the protein digestion solution by adopting a chromatographic column and performing desalination, endotoxin removing, sterile filtration and storage. The preparation method is simple and can be used for preparing recombinant staphylococcus aureus vaccine in a large scale.

The present invention provides a thermostable duplex-specific nuclease (DSN) and a method for digesting a nucleic acid using said DSN, namely, a protein having a Brachyura-derived duplex-specific nuclease activity, a gene encoding for said protein, a recombinant vector comprising said gene and a transformed cell or transduced cell comprising said vector and a method for producing a protein having a duplex-specific nuclease activity, which comprises culturing said transformed cell or transduced cell using a medium and collecting the protein having a duplex-specific nuclease activity from the cultured mixture, a method for digesting a nucleic acid using said protein having duplex-specific nuclease activity, a method for detecting RNA using said DSN and a reagent kit to be used in the aforementioned methods.

Concerning a fermented alcoholic beverage using beer yeast and a method of producing the same, it is intended to provide a fermented alcoholic beverage having a natural color level and a flavor like beer imparted thereto and a process for producing the same. By controlling the liquid color and the flavor of a fermented alcoholic beverage with the use of a Maillard reaction product obtained from a sugar and a protein digestion product and a preparation of the same, a fermented alcoholic beverage having a natural color level and a flavor like beer imparted thereto can be produced without resorting to a coloring agent such as caramel. This method of producing a fermented alcoholic beverage is applicable to sparkling liquors and miscellaneous liquors the color level and flavor of which as fermented alcoholic beverages should be reinforced due to restricted fermentation materials.

The invention relates to a feed, in particular to a powdered oil feed with whey powder and a preparation method thereof, which mainly solve the technical problems that in the prior art, protein, whey powder and oil all in the conventional feed are simply mixed and stirred, so that oil and protein digestion utilization of animals is low, the protein, the whey powder and the oil carry bacterial viruses easily and insanitation is caused, the storage time is short, the application is poor and the like. According to the preparation method, whey powder, plant protein, animal protein and plant oil are mixed in proportion, added into water and heated, then emulsifier is added, and the mixture is fully stirred and then gets into an emulsifying machine for treatment, the homogenization treatment is performed to the product subjected to emulsification through a homogenizer, spray drying is performed to the product through the variable frequency drying technology and via a spray drying tower, and the dried mixture is subjected to sieving, metering and packing, so that a finished product is obtained.

The invention discloses a pretreatment method for directly detecting infection urine pathogen by MALDI-TOF MS. An urine sample pretreatment part is improved on a methodology, bacteria is washed and collected, formic acid and acetonitrile are used for performing protein digestion on bacteria, and then the detection is carried out, thereby the bacteria identification time is substantially shortened and the identification accuracy is enhanced. The pretreatment method is capable of rapidly and accurately identifying the infection urine sample, and can timely guide the clinical medication with pertinence.

The invention concerns a method of reducing body fat content of a subject in need thereof, the method comprising administering to the subject an agent capable of down-regulating activity and / or expression of at least one component participating in protein digestion and / or absorption. Such agents may be (i) an oligonucleotide directed to an endogenous nucleic acid sequence expressing said at least one component participating in said protein digestion and / or absorption or (ii) a protease inhibitor directed to said at least one component participating in protein digestion and / or absorption. The invention is particularly directed to a method of reducing body fat content of a subject in need thereof, the method comprising administering to the subject serine protease inhibitor inhibiting both enteropeptidase and trypsin activity.

The invention provides high-protein and full-nutrition seven-day meal replacement milkshake. The milkshake is prepared from the following raw materials in parts by mass: 20-50 parts of protein powder,10-30 parts of milk powder, 5-15 parts of comprehensive fruit and vegetable powder, 1-5 parts of compound mineral powder, 5-10 parts of prebiotics, 50-100 parts of satiety powder, 0.1-0.8 part of conjugated linoleic acid glyceride, 3-10 parts of fruit powder and 2-8 parts of fruit granules. The meal replacement milkshake provided by the invention can supplement the proteins, vitamins and mineralswhich are required for daily nutrition of a human body, and is balanced and comprehensive in nutrition; on the basis, due to the optimal combination of protein ratios, the protein digestion rate is slowed down; since the prebiotics are scientifically proportioned, the intestinal flora can be adjusted, the intestinal motility is enhanced, and the metabolism is accelerated; by adopting the low-carbon, low-fat and high-fiber dietary collocation, high satiety and low intake are realized; the diverse tastes of seven different meals for seven days can satisfy the taste buds. The meal replacement milkshake has the characteristics of being low in energy, high in protein, rich in minerals and vitamins, and the like. The product is good in taste, strong in satiety, comprehensive in nutrition and significant in weight reducing effect.

The present invention relates to a fermented infant formulae for decreasing protein digestive effort by decreasing the amount of endogenously formed proteases, concomitant with an increased protein digestion. The invention also relates to low protein fermented infant formulae.

The invention provides reagents and methods for the accurate quantification of proteins in complex biological samples. Quantification is obtained by adding to a sample a peptide combo, which is essentially a collection of synthetic reference peptides. The synthetic reference peptides have a small mass difference when compared to the biological reference peptides that originate upon digestion from the proteins present in the sample. Reference peptides and synthetic reference peptides are selected and the identity and accurate amounts of reference peptides are determined by mass spectrometry. The methods can be used in high throughput assays to interrogate proteomes.

The invention relates to a feed for increasing laying hen fertilization rate and laying rate, which comprises a feed main material and a traditional Chinese medicine additive, wherein the main material comprises the following raw materials by weight: 50-60 parts of corn, 25-30 parts of soybean meal, 4-6 parts of fish meal, 5-7 parts of wheat bran, 4-6 parts of shell powder, 2-3 parts of bone meal, 3-5 parts of trace elements, 3 parts of compound vitamin, and 2 parts of lysine; the weight part of the traditional Chinese medicine additive is 1, and the traditional Chinese medicine additive comprises isatis roots, radix astragali, epimeddium, venenum bufonis, bezoar, borneol, gypsum, glutinous rehmannia, buffalo horn, coptis, fructus forsythia, and balloonflower roots. The feed is not only added with trace elements such as nitrogen, phosphorus, calcium and the like, but also added with trace of pyridoxine algae on the basis of general feed; pyridoxine has promotion effect on internal protein digestion and absorption of laying hens, so absorption of amino acids by laying hens is promoted, and thus disease resistance of laying hens is improved; during feeding, the rates of diseased hens and dead hens are decreased by 50% and 80% respectively.

The invention provides a method for producing rice bran proteins by an enzyme hydrolysis method, and relates to a method for producing protein powder. Defatted rice bran is used as raw materials for extracting and preparing rice bran proteins. The method comprises the steps of raw material pretreatment, mixing enzymolysis, pH regulation, protein digestion, centrifugation, spraying drying and rice bran protein obtaining. The rice bran protein extraction rate reaches 76.19 percent, and the purity of the obtained rice bran proteins reaches higher than 70 percent. The method has the advantages that the extraction efficiency is high; the process is simple; the method is reliable; the stability is good; a path is provided for the integral utilization of rice bran resources and the preparation of the rice bran proteins.

Reagents are disclosed for chemoselective tagging of methionine residues in peptides and polypeptides, subsequent bioorthogonal tag functionalization, and cleavage of the tags when desired to regenerate unmodified samples. This method compliments other peptide tagging strategies and adds capability for tag removal, which may be useful for release of therapeutic peptides from a carrier, or release of tagged protein digests from solid supports.

The invention discloses a preparation method of a grain-albumin-free soy sauce, and the grain-albumin-free soy sauce, and relates to the technical field of soy sauce brewing. The grain-albumin-free soy sauce is prepared from beans, bean pulp, aspergillus oryzae, table salt, water and yeast, grain raw materials such as wheat are not added into production raw materials of the soy sauce, the producedsoy sauce does not contain a grain albumin allergen, the quality index and nutrients both meet the quality standard requirements of an existing soy sauce, and the grain-albumin-free soy sauce can replace the existing soy sauce to meet the edible needs of the crowd allergic to grain albumin; and the protein content of the produced soy sauce can reach 15 g / 100mL and is higher than the existing soysauce by 50%, the content of soluble non-salt solid matter is increased by about 30% than that of the current soy sauce, the nutritional value is higher, the sauce flavor is thicker, the grain-albumin-free soy sauce can serve as the soy sauce special for Japanese food such as sushi and sashimi, and the food taste is enough to be wrapped. The invention further provides a production method of the grain-albumin-free soy sauce, and the protein digestion rate of a production process is increased by about 10% than that of an existing process.

Processes for increasing rumen undegraded protein in protein containing compositions, fermentation by-products or combinations thereof are disclosed. Uses of alkaline crystalline solids to increase rumen undegraded protein in protein containing compositions, fermentation by-products or combinations thereof are further disclosed. Products produced from such processes are also disclosed.

The invention provides a pig feed and a preparation method thereof. The pig feed comprises 1 percent by weight of premix, a synthetic amino acid and a compound enzyme, wherein the synthetic amino acid is a mixture of L-lysine, DL-methionine, L-threonine and L-tryptophan; the compound enzyme is a mixture of protease, amylase, phytase, xylanase and beta-mannase. Compared with a common pig feed, the daily pig feed prepared from the synthetic amino acid and the compound enzyme has the advantages that the daily grain and the conversion efficiency of the feed are obviously increased, and fecal nitrogen and urine nitrogen are obviously reduced; the growth speed of pigs is increased, and the environmental improvement is facilitated; the growth of animals is not affected by the added synthetic amino acid; the reduction of the crude proteins of the daily pig feed facilitates the development of intestinal tracts, the villus height of duodenum in the intestinal tracts is increased, and the digestion and the absorption of proteins are facilitated.

The invention discloses feed for preventing desquamation bleeding of megalobrama amblycephala in a high-density breeding condition. The feed comprises the following components, by mass percentage: 14.8 of wheat, 15.0 of rice bran, 0.2 of choline chloride, 0.2 of betaine, 10.0 of corn germ dreg, 8.0 of starch maize slag, 18.0 of soybean meal, 6.0 of cottonseed meal, 10.0 of rapeseed meal, 4.0 of chicken meal, 9.0 of fish meal, 1.8 of calcium dihydrogen phosphate, 1.0 of soya-bean oil, 0.5 of lard, 0.5 of a Chinese herbal medicine preparation, and 1.0 of premix, and the total amount of the components is 100%. According to the invention, the Chinese herbal medicine preparation is added in the feed, and the amount of vitamin K3, vitamin C, and vitamin E is added in the feed formula, so that the nutrition metabolism function of the body is effectively enhanced; metabolism and fat conversion of the body are improved; the viscosities of the fat in the blood and the liver are effectively reduced; and conversion and absorption of the feed are improved. The feed has functions of invigorating the stomach and promoting digestion, protecting the liver and the gallbladder, resisting bacteria and virus, and promoting fat conversion and protein digestion and absorption; the stress resistance and immunity of the megalobrama amblycephala are improved; the survival rate is increased; and desquamation bleeding of megalobrama amblycephala is effectively prevented.

The invention discloses an extraction method of auricularia auricula polysaccharide. The extraction method comprises the following specific steps: carrying out superfine grinding on auricularia auricula to obtain auricularia auricula superfine powder; adding water into the auricularia auricula superfine powder, and adding yeast lysing enzymes to carry out enzymolysis and lysing, so as to obtain anauricularia auricula enzymatic hydrolysate and an auricularia auricula residue; adding water into the auricularia auricula residue, and carrying out microwave extraction to obtain an auricularia auricula extracting solution; combining the auricularia auricula enzymatic hydrolysate and the auricularia auricula extracting solution, carrying out filtering, adding protease for enzymolysis, carrying out centrifuging, and removing proteins of a supernate by using a Sevage method to obtain a deproteinized polysaccharide solution; and concentrating the deproteinized polysaccharide solution, then precipitating polysaccharide by using ethanol, and carrying out centrifuging, precipitating and freeze-drying to obtain the auricularia auricula polysaccharide. The extraction method provided by the invention is simple and feasible, the extraction rate and purity of the auricularia auricula polysaccharide are high, the auricularia auricula polysaccharide has good application values and market potential; and dissociative and zymolytic protein in a polysaccharide liquid can be effectively removed, so that the activity of the polysaccharide is kept from being damaged.

The invention provides a microfluidic system and method to rapidly analyze large numbers of compounds or complex mixtures of compounds, particularly, low abundance cellular proteins involved in cell signaling pathways. In one aspect, an integrated microfluidic system comprises an upstream separation module (preferably, a multi-dimensional separation device), a microfluidic device for on-device protein digestion of substantially separated proteins received from the upstream separation module, a downstream separation module for separating digestion products of said proteins, a peptide analysis module and a processor for determining the amino acid sequence of said proteins. Preferably, the system comprises an interfacing microfluidic device between the downstream separation module and the peptide analysis module.

The invention provides reagents and methods for the accurate quantification of proteins in complex biological samples. Quantification is obtained by adding to a sample a peptide combo, which is essentially a collection of synthetic reference peptides. The synthetic reference peptides have a small mass difference when compared to the biological reference peptides that originate upon digestion from the proteins present in the sample. Reference peptides and synthetic reference peptides are selected and the identity and accurate amounts of reference peptides are determined by mass spectrometry. The methods can be used in high throughput assays to interrogate proteomes.

The invention discloses soybean milk powder and a preparation method thereof, salty soybean milk powder prepared by using the soybean milk powder, and salty soybean milk. The preparation method of thesoybean milk powder comprises: 1) immersing peeled soybeans in 25-35 DEG C water, soaking for 2-4 h, carrying out wet grinding to obtain a slurry, and separating the slurry and the residue, wherein the temperature of the water for grinding is 80-90 DEG C; 2) carrying out deodorization, enzyme inactivation and sterilization, carrying out enzymolysis on the soybean milk supernatant for 0.5-1 h at atemperature of 40-55 DEG C, carrying out centrifugation, and carrying out three-effect concentration on the separated soybean milk supernatant, wherein the first-effect concentration temperature is 60-75 DEG C, the evaporation vacuum degree is 0.055-0.065 MPa, the second-effect concentration temperature is 50-63 DEG C, the evaporation vacuum degree is 0.065-0.070 MPa, the third-effect concentration temperature is 42-55 DEG C, the evaporation vacuum degree is 0.075-0.095 MPa, and the solid content of the discharged concentrated material is 15-25%; and 3) carrying out spray drying on the concentrated material, collecting the power, cooling, and packaging. According to the present invention, the soybean milk powder has characteristics of easy brewing, no odor, no bean flavor, good taste andhigh protein digestion and absorption rate; and with the salty soy milk, people can enjoy the delicious taste of the soybean milk and can chew the fresh fragrance of vegetables.

The invention relates to a dried ampelopsis grossedentata konjac and a preparation method thereof. The main ingredients of the dried ampelopsis grossedentata konjac comprise ampelopsis grossedentata and konjac glucomannan. The ampelopsis grossedentata contains 19 kinds of essential nutrients and trace elements for human bodies, having functions of preventing of such diseases as cardiovascular disease, osteoporosis, cancer, and the like; inhibiting of Gram-negative bacteria, influenza A virus, and influenza B virus; and no influence on protein digestion and absorption because the ampelopsis grossedentata does not contain tannic acid, and being suitable to eat by people with anemia. Konjac has such benefits as detoxifying, weight losing, blood sugar reducing, blood pressure lowering, cancerpreventing, and the like. Complex gum formed by Konjac gum and xanthan gum is used as an encapsulating wall material, protecting ingredient activity of the ampelopsis grossedentata. The dried ampelopsis grossedentata konjac provided by the invention has the advantages of easily obtained raw materials, low price, safety and antibacterial property which totally meet the requirements of the nationalrelated standards, and more abundant nutrition compared with the common leisure foods. Besides, the dried ampelopsis grossedentata konjac has such health care functions as weight losing, inflammationdiminishing, deficiency fire reducing, blood sugar reducing, blood pressure lowering, appearance improving, sleep improving, and the like.