A method for preparing a giant
phospholipid vesicle by using a finger-like
microelectrode relates to a preparation method for the giant
phospholipid vesicle. The method for preparing the giant
phospholipid vesicle by using the finger-like
microelectrode is realized by the following technical scheme: firstly, sequentially carrying out ultrasonic cleaning on a finger-like mircroelectrode with absolute ethyl
alcohol and
distilled water,
drying the finger-like mircroelectrode by blowing with
nitrogen gas and treating the dried finger-like mircroelectrode through a
plasma cleaning
machine for later use; secondly, dissolving phospholipid into
chloroform to prepare a phospholipid solution, tiling the phospholipid solution on the finger-like mircroelectrode and carrying out
vacuum drying on the finger-like mircroelectrode; thirdly, assembling the dried finger-like mircroelectrode coated with phospholipid, a polyfluortetraethylene rectangular frame and
cover glass to form a device, injecting a
sucrose or glucose solution in the device and placing the device on a heating plate; and fourthly, respectively connecting the
electrode with a
signal generator, setting the
voltage and the frequency and forming the giant phospholipid vesicle. The method disclosed by the invention has the advantages of small
electrode area, mild
reaction conditions, uniformity in size, favorable
controllability of the generated giant phospholipid vesicle and the like.