Biosensor interface constructed through phospholipid membrane layer and preparation method of interface

Abstract

The invention discloses a biosensor interface constructed through a phospholipid membrane layer. The biosensor interface can effectively hinder nonspecific adsorption, introduction of a sealing agent is avoided, a bionic environment capable of protecting activity is provided for the subsequent molecular recognition process, and the requirement of a biosensor for a sensing interface is met. The biosensor interface comprises 1-decanethiol mixed with phospholipid vesicles, DPPC (dipalmitoylphosphatidylcholine) used for forming single-layer phospholipid vesicles, cholesterol used for forming a phospholipid modified layer as well as a biosensing surface, wherein the biosensing surface is a transparent solid substrate plated with a 50-nm gold membrane, a phospholipid mixture mixed with the 1-decanethiol fixes the single-layer phospholipid vesicles on the biosensing surface by the aid of sulfhydryl groups of the 1-decanethiol. The invention further provides a preparation method.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and in particular relates to a biosensor interface constructed by a phospholipid film layer and a method for preparing the biosensor interface. Background technique [0002] The phospholipid membrane layer is the basic unit of the cell membrane. It has the characteristics of high biomimeticity and no protein adsorption, and is an ideal material for constructing the interface of biosensors. However, in the traditional construction method, the commonly used gold substrate at the interface between the phospholipid film and the biosensor is bonded by hydrophilic force, and the structure is unstable, so it is not suitable for the sensing interface of the biosensor. Moreover, it is necessary to introduce a blocking agent, which cannot block non-specific adsorption, cannot provide a biomimetic environment that can protect the activity, is complicated to operate, has many chemical synthesis processes,...

AI Technical Summary

Problems solved by technology

However, in the traditional construction method, the commonly used gold substrate at the interface between the phospholipid film layer and the biosensor is bonded by hydrophilic force, and the structure is unstable, so it is not suitable for the sensing interface of the biosensor.

Moreover, it is necessary to introduce a blocking agent, which cannot block non-specific adsorption, cannot provide a biomimetic environment that can protect the activity, is complicated to operate, has many chemical synthesis processes, and cannot protect the fluidity of phospholipid molecular vesicles.

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