Method for preparing giant phospholipid vesicle by using finger-like microelectrode

A giant phospholipid and microelectrode technology, which is applied in the field of preparation of giant vesicles, can solve the problem of electrode non-coplanar electrode area, etc., and achieve the effect of small electrode use area, high yield, and good vesicle shape

Inactive Publication Date: 2012-11-21
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the electrodes used in the current electroforming method mainly use a double-facing electrode system. The electrodes themselves mainly have rod-shaped, planar or mesh structures. The electrodes are not coplanar and the electrode area is relatively large.

Method used

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  • Method for preparing giant phospholipid vesicle by using finger-like microelectrode
  • Method for preparing giant phospholipid vesicle by using finger-like microelectrode
  • Method for preparing giant phospholipid vesicle by using finger-like microelectrode

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Experimental program
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specific Embodiment approach 1

[0015] Specific embodiment 1: This embodiment prepares giant phospholipid vesicles according to the following method:

[0016] 1) Preparation and cleaning of electrodes. The prepared finger-shaped microelectrodes (the width of the interdigital fingers are 50~200μm) are ultrasonically cleaned with absolute ethanol and distilled water for 5~15min, then dried with nitrogen, and treated by a plasma cleaner for 20~30s for later use.

[0017] 2) Application and drying of phospholipids. The phospholipid (egg PC) is dissolved in chloroform (99.95v.%) to prepare a 2-20mg / mL phospholipid solution. Take 2-10μL of the phospholipid solution on the finger-shaped microelectrode, and apply the syringe needle back and forth 2-6 times. , To make it evenly spread on the finger-shaped microelectrode, and then put it in a vacuum dryer and vacuum dry for 2 hours to completely remove the residual chloroform solvent.

[0018] 3) Forming the assembly of the device. The dried finger-shaped microele...

specific Embodiment approach 2

[0020] Specific embodiment 2: This embodiment prepares giant phospholipid vesicles according to the following method:

[0021] 1) Preparation and cleaning of electrodes.

[0022] (1) ITO surface cleaning: ITO conductive glass is ultrasonically cleaned with ethanol and distilled water for 15 minutes, N 2 Blow dry, treat by plasma cleaning machine for 30s, and place on a heating plate to preheat at 110°C for 10 minutes.

[0023] (2) Laminator (GMP Photonex-Sync235) is set to preheat at 110℃, AM175 photoresist is pressed tightly on the ITO substrate to form a film, and the finger electrode mask is placed, and exposed and developed by soft UV (365nm) for 2s, 65℃ Heat and cure for 4min, with a mass percentage of 1.5% Na 2 CO 3 The solution was sonicated for 7 seconds and then washed, and the finger electrode pattern was initially obtained.

[0024] (3) The AUTOLAB PGSTAT302N electrochemical workstation adopts chronoamperometry to selectively corrode the finger electrodes. A hydrochloric a...

specific Embodiment approach 3

[0030] Specific embodiment 3: This embodiment prepares giant phospholipid vesicles according to the following method:

[0031] 1) Preparation and cleaning of electrodes. The prepared 50μm finger electrode was ultrasonically cleaned with absolute ethanol and distilled water for 10 minutes, then dried with nitrogen, and treated by a plasma cleaner for 20 seconds for later use.

[0032] 2) Application and drying of phospholipids. The phospholipid (egg PC) was dissolved in chloroform (99.95%) to prepare a 15mg / mL phospholipid solution, and 5μL of the phospholipid solution was applied to the finger microelectrode, and the syringe needle was used to apply back and forth 4 times to make it evenly spread on the The finger-shaped microelectrodes are then placed in a vacuum desiccator and dried under vacuum for 2 hours to completely remove the residual chloroform solvent.

[0033] 3) Forming the assembly of the device. The dried finger-shaped microelectrode coated with phospholipids...

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Abstract

A method for preparing a giant phospholipid vesicle by using a finger-like microelectrode relates to a preparation method for the giant phospholipid vesicle. The method for preparing the giant phospholipid vesicle by using the finger-like microelectrode is realized by the following technical scheme: firstly, sequentially carrying out ultrasonic cleaning on a finger-like mircroelectrode with absolute ethyl alcohol and distilled water, drying the finger-like mircroelectrode by blowing with nitrogen gas and treating the dried finger-like mircroelectrode through a plasma cleaning machine for later use; secondly, dissolving phospholipid into chloroform to prepare a phospholipid solution, tiling the phospholipid solution on the finger-like mircroelectrode and carrying out vacuum drying on the finger-like mircroelectrode; thirdly, assembling the dried finger-like mircroelectrode coated with phospholipid, a polyfluortetraethylene rectangular frame and cover glass to form a device, injecting a sucrose or glucose solution in the device and placing the device on a heating plate; and fourthly, respectively connecting the electrode with a signal generator, setting the voltage and the frequency and forming the giant phospholipid vesicle. The method disclosed by the invention has the advantages of small electrode area, mild reaction conditions, uniformity in size, favorable controllability of the generated giant phospholipid vesicle and the like.

Description

Technical field [0001] The invention relates to a method for preparing giant vesicles, in particular to a method for preparing giant phospholipid vesicles by electroforming using planar finger-shaped microelectrodes. Background technique [0002] The current methods for preparing giant vesicles mainly include hydration method, reverse phase evaporation method, fusion method, electroformation method, etc. Among them, the electroforming method has the advantages of short preparation cycle, fast speed, high controllability, simple operation, mild reaction conditions, and low environmental pollution. However, the electrode used in the current electroforming method mainly adopts a double-sided counter electrode system. The electrode itself mainly has a rod-shaped, flat or mesh structure, and the electrodes are not coplanar and the electrode area is relatively large. Summary of the invention [0003] The purpose of the present invention is to provide a method for preparing giant phosph...

Claims

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Application Information

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IPC IPC(8): B01J19/08
Inventor 韩晓军毕洪梅刘丹青
Owner HARBIN INST OF TECH
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