Preparation method of eukaryotic cell model

A eukaryotic cell and model technology, applied in biochemical equipment and methods, microorganisms, tissue culture, etc., can solve problems that do not satisfy the research of life evolution, achieve the effect of uniform size, complete structure, and functionalization

Active Publication Date: 2016-01-20
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims to solve the problem that the existing cell models only simulate prokaryotic cells and are not satisfied with the research of life evolution, and provide a preparation method of eukaryotic cell models

Method used

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  • Preparation method of eukaryotic cell model
  • Preparation method of eukaryotic cell model
  • Preparation method of eukaryotic cell model

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Experimental program
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specific Embodiment approach 1

[0027] Specific embodiment one: the preparation method of the eukaryotic cell model of this embodiment is carried out according to the following steps:

[0028] 1. Preparation of unicellular phospholipid vesicles: add dimyristoylphosphatidylcholine and cholesterol to chloroform to obtain a phospholipid solution, and coat the surfaces of two ITO glass electrodes with 5-7 μL of phospholipid solution respectively, and wait for the chloroform to evaporate to dryness , to obtain two ITO glass electrodes whose surfaces are covered with phospholipid solution;

[0029] Place the polytetrafluoroethylene rectangular frame between two ITO glass electrodes covered with phospholipid solution to obtain the electroforming device, use vacuum grease to seal the electroforming device, and the polytetrafluoroethylene rectangular frame and the two surfaces are covered with phospholipid The ITO glass electrode of the solution forms a middle cavity, and fills the PCR buffer solution in the middle c...

specific Embodiment approach 2

[0034] Embodiment 2: This embodiment differs from Embodiment 1 in that the mass ratio of dimyristoylphosphatidylcholine to cholesterol in step 1 is (7-4):(3-1). Others are the same as in the first embodiment.

specific Embodiment approach 3

[0035] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: the concentration of dimyristoylphosphatidylcholine in the phospholipid solution in step 1 is 3-5 mg / mL. Others are the same as in the first or second embodiment.

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Abstract

The invention provides a preparation method of an eukaryotic cell model, and relates to a preparation method of cell models. The problems that an existing cell model only simulates an eukaryotic cell, and research of life evolution is not satisfied are solved. The method comprises the steps that 1, a single-chamber phospholipid vesicle is prepared; 2, an artificial eukaryotic cell model is prepared; 3, PCR amplification is conducted; 4, artificial cell division is conducted. According to the preparation method of the eukaryotic cell model, an artificial eukaryotic cell of which the structure is complete, the size is uniform, and the size of a small vesicle is close to that of a human body cell nucleus is prepared, a PCR technology and a fission process are conducted in the artificial cell nucleus successfully, and the functionalization of the artificial cell nucleus is achieved. The preparation method of the eukaryotic cell model is used for preparing the eukaryotic cell model.

Description

technical field [0001] The invention relates to a preparation method of a cell model. Background technique [0002] Explore the origin of life, study the evolution of organisms, and construct a model of structure, shape and performance, which is more conducive to clarifying the interaction between cells and the relationship between function and structure and form. This is exactly what building a cell model is all about. [0003] At present, the establishment of cell models using liposomes only simulates prokaryotic cells. However, prokaryotic cells are cells that make up prokaryotic organisms. These cells do not have a nucleus bounded by a nuclear membrane, but only nucleoids, which have a low evolutionary status. Eukaryotic cells evolved gradually from a prokaryotic cell through natural selection and mutation. In order to study the evolution process of life, it is of great significance to establish eukaryotic cell models. Contents of the invention [0004] The inventio...

Claims

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Application Information

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IPC IPC(8): C12N5/00
Inventor 韩晓军宗薇
Owner HARBIN INST OF TECH
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