Lipidosome-protected nano-gold gene vector and preparation method thereof
A gene carrier and nano-gold technology, applied in the field of gene carriers, can solve the problems of inability to release the target gene, inability to express the target gene, slow release of the target gene, etc. Effect
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[0041] The invention discloses a preparation method of a liposome-protected nano-gold gene carrier, which comprises the following steps:
[0042](A) Dissolving di-octadecyldimethylammonium bromide and dioleoylphosphatidylethanolamine in an organic solvent, then removing the organic solvent, dissolving in water, and ultrasonically treating to obtain a phospholipid vesicle solution;
[0043] The molar ratio of dioctadecyldimethylammonium bromide to dioleoylphosphatidylethanolamine is 1:10~10:1;
[0044] (B) After the phospholipid vesicle solution is mixed with a compound containing gold ions, a reducing agent is added to react to obtain a liposome-protected nano-gold gene carrier.
[0045] In the present invention, dioctadecyldimethylammonium bromide and dioleoylphosphatidylethanolamine are dissolved in an organic solvent first, then the organic solvent is removed and dissolved in water, and the phospholipid vesicle solution is obtained by ultrasonication. The molar ratio of di...
Embodiment 1
[0050] Dissolve 0.0032g of DODAB and 0.0018g of DOPE in 10mL of chloroform, remove most of the chloroform with nitrogen flow, and then vacuum dry for 2 hours to remove traces of chloroform. After adding 7 mL of aqueous solution, sonicate until clarified to prepare DODAB / DOPE vesicle solution. Then, 7 μl of 0.1M chloroauric acid solution was added to the prepared vesicle solution and stirred to mix. After mixing, 8 μl of 0.4 M sodium borohydride solution was added. DODAB / DOPE-protected gold nanoparticles were obtained after stirring for 1 hour.
[0051] Remove 500 µl and record the UV-Vis absorption spectrum at room temperature, see figure 1 , figure 1 It is the ultraviolet absorption spectrum of the nano gold gene carrier that the liposome protection that embodiment 1 obtains. figure 2 Electron micrograph of the nano-gold gene carrier protected by the liposome prepared in Example 1. Depend on figure 1 and figure 2 It can be seen that the present invention has obtained...
Embodiment 2
[0054] Dissolve 0.0032g of DODAB and 0.0037g of DOPE in 10mL of chloroform, remove most of the chloroform with nitrogen flow, and then vacuum dry for 2 hours to remove traces of chloroform. After adding 10 mL of aqueous solution, sonicate until clarified to prepare a DODAB / DOPE vesicle solution. Then, 10 microliters of 0.1M chloroauric acid solution was added to the prepared vesicle solution and stirred to mix. After mixing, 12 microliters of 0.4M sodium borohydride solution was added. DODAB / DOPE-protected gold nanoparticles were obtained after stirring for 1 hour.
[0055] Take out 500 µl and record the UV-Vis absorption spectrum at room temperature. see Figure 4 , Figure 4 The UV absorption spectrum of the liposome-protected nano-gold gene carrier obtained in Example 2. Figure 5 Electron micrograph of the nano-gold gene carrier protected by the liposome prepared in Example 2. Depend on Figure 4 and Figure 5 It can be seen that the present invention has obtained ...
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