Tissue factor-loaded calcium carbonate particle self-propelled hemostatic dressing

A tissue factor and hemostatic dressing technology, applied in the fields of application, medical science, pharmaceutical formulation, etc., can solve the problem of insufficiency of wounds, and achieve the effects of easy removal, avoidance of secondary damage, and high coagulation ability.

Inactive Publication Date: 2021-08-17
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there are many kinds of hemostatic dressings in various forms, but most of them have some conditions that cannot be satisfied for the wound

Method used

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  • Tissue factor-loaded calcium carbonate particle self-propelled hemostatic dressing
  • Tissue factor-loaded calcium carbonate particle self-propelled hemostatic dressing
  • Tissue factor-loaded calcium carbonate particle self-propelled hemostatic dressing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Expression and purification of tissue factor

[0027] A plasmid (pET-22b) carrying the tissue factor gene was transformed into bacterial cells. Place the cells in medium containing ampicillin and grow at 37 °C until A 600 Reach 0.6-0.8. The culture was then moved to 25°C and induced overnight with isopropyl-β-D-1-thiogalactopyranoside. The culture was centrifuged to obtain a pellet and suspended with Triton X-100 reagent. After lysing with a French press and centrifugation, the supernatant was collected into a new tube. Next, expressed tissue factor was purified from the supernatant by Ni-affinity chromatography in the presence of Tween 80.

[0028] (2) Preparation of tissue factor-integrated liposomes

[0029] The mixture of DOPC and DOPS was dried in a glass bottle and dissolved in Tris-HCl containing deoxycholate and tissue factor. The samples were stirred with the biobeads for 90 min at room temperature before adding part of the biobeads removal detergent ...

Embodiment 2

[0041] This example is the same as Example 1 except that the final concentration of tissue factor in step (3) is 3 nM, and other steps are the same.

Embodiment 3

[0043] This example is the same as Example 1 except that the final concentration of tissue factor in step (3) is 9 nM, and other steps are the same.

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PUM

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Abstract

The invention discloses a method for preparing and producing a hemostatic dressing embedded with a tissue factor by taking calcium carbonate as a raw material. The method comprises the following steps: (1) expressing and purifying in bacteria to obtain the tissue factor with biological activity; (2) treating phospholipid and the tissue factor obtained in the step (1) to obtain a combination of the tissue factor and phospholipid vesicles; (3) adding the tissue factor-vesicle combination obtained in the step (2) into a calcium chloride solution, fully and uniformly mixing, adding a sodium carbonate solution, and carrying out post-treatment to obtain CaCO3 particle powder; and (4) uniformly mixing the CaCO3 particle powder obtained in the step (3) with acid powder to obtain the hemostatic dressing with the self-propelling capability. The tissue factor is a protein with biological activity and efficient blood coagulation capability, and can start a human body blood coagulation cascade exogenous pathway to stop bleeding. The prepared hemostatic dressing is in the form of powder, has self-pushing capacity, can push tissue factors to the depth of a wound, is suitable for irregular wounds which have certain depth and cannot effectively stop bleeding by a traditional dressing, and is wide in application environment. By adding the tissue factors, the hemostatic dressing can play a more active role in wound management, the passive hemostatic effect of a traditional hemostatic dressing in wound management is replaced, the biocompatibility is better, the degradability is high, and the risk of secondary damage to the wound can be reduced.

Description

technical field [0001] The invention relates to a hemostatic dressing with pushing ability constructed by using calcium carbonate microparticles as a carrier and loading tissue factor with blood coagulation bioactivity into the calcium carbonate microparticles. Background technique [0002] In clinical (such as surgical and dental operations, severe epistaxis, postpartum hemorrhage, and hemophilia), uncontrolled bleeding may occur, and the first task during uncontrolled bleeding is to effectively control bleeding to prevent life-threatening complications such as hemorrhagic shock . The risk of uncontrolled bleeding can be avoided by rapid delivery of therapeutic agents (such as coagulants, antifibrinolytics, or growth factors) to damaged vascular tissue. Since some irregular wounds have a certain depth, there is a certain distance between the bleeding source and the skin surface, and the therapeutic agent cannot effectively reach the bleeding source through the injection sy...

Claims

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Application Information

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IPC IPC(8): A61L26/00A61L24/00A61L24/02A61L24/10
CPCA61L26/0028A61L26/0004A61L26/0066A61L26/0061A61L24/10A61L24/02A61L24/001A61L24/0015A61L2300/418A61L2300/252A61L2400/04C08L89/00
Inventor 刘承琨石壮王小强黄方
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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