32 results about "Phenol oxidase activity" patented technology

The invention discloses a method and specific primers of detecting the character of the activity of wheat polyphenol oxidase. The primers provided by the invention for detecting the character of the activity of wheat polyphenol oxidase is a pair of primers consisting of the nucleotide sequences of the sequence 1 and the sequence 2 in the sequence table. The method of detecting the character of the activity of wheat polyphenol oxidase is that PCR amplification can be conducted by taking the DNA of a to-be-detected gene group as a templet and taking the nucleotide sequences of the sequence 1 and the sequence 2 in the sequence table as primers; and then the amplified outcomes can be detected to see whether 750 to 1000 bp of bands exist. The method and specific primers can play an important role both in the detection of the character of the activity of wheat polyphenol oxidase and in the breeding.

The invention relates to the technical field of enzyme activity, in particular to an apple polyphenol oxidase activity promoter and application thereof. The apple polyphenol oxidase activity promoter of the present invention comprises lauryltrimethylammonium bromide or hexadecyltrimethylammonium bromide and Gemini surfactant in a weight ratio of 10~2000:1~4 The mixture that mixes in proportion, described Gemini surfactant is dibromide-bis(dimethyldodecyl)pentammonium or dibromide-bis(dimethylhexadecyl)pentammonium. The present invention also includes the application of the above accelerator to improve the activity of apple polyphenol oxidase, which can increase the activity of apple polyphenol oxidase to 240%, and the consumption level of the accelerator is low.

The invention discloses a processing method of sulfur-free konjac chips, which comprises the following steps: 1) washing the konjac; 2) rubbing the konjac corms; 3) slicing and collecting the konjac corms; 4) 1. Use steam to blanch the konjac chips to inactivate enzymes to inhibit the activity of polyphenol oxidase; 5) process the konjac chips with konjac flour so that the surface of the konjac chips is coated with a layer of konjac flour; 6) konjac chips Carrying out microwave drying The object of the present invention is to provide a kind of processing method of sulfur-free konjac slices, use fresh konjac as raw material, through specific processing method, obtain sulfur-free konjac slices.

The invention relates to a preparation method and application of a prawn non-specific immunity reinforcing agent oligopeptide. The preparation method comprises the following steps of: mincing pacific alaskapollack dogmeat by a meat mincer and then placing in an enzyme reactor to heat and sterilize; triturating and then heating to 90 DEG C to preserve the temperature; sterilizing for 20 minutes in a constant temperature water bath; adding water and protease preparations to mix; carrying out enzymolysis under the condition of optimized enzymolysis process parameters; then heating enzymolysis liquid so that protease is inactivated; roughly filtering fishbones and centrifugalizing the enzymolysis liquid to obtain a soluble protein oligopeptide; ultrafiltering, purifying, freezing and drying and then obtaining protein oligopeptide powder. The protease preparations comprise Alcalase and Flavourzyme, and the enzymolysis process parameters comprise the enzymolysis temperature of 55 DEG C, the initial pH value of 8.5, the material and water ratio of 1:1 and the enzymolysis time of 3 hours. A macromolecule substance is intercepted by using a method for combining 8,000rpm high-speed centrifugalization and 100kDa ultrafiltration membrane ultrafiltrating purification, and 30 percent of protein oligopeptide with the molecular weight of smaller than 100Da is obtained, the further filteration is carried out by 1kDa / 5kDa ultrafiltration membrane to obtain an ultrafiltrate, and the oligopeptide powder is obtained by freezing and drying the ultrafiltrate. The oligopeptide powder replaces 5 percent of fish meal in prawn feed and can obviously improve the blood corpuscle density, the phenoloxidase activity, the respiratory burst activity, the SOD activity, the NOS activity and the specific growing rate of a prawn.

The invention discloses a preparation method of phenol oxidase active protein. The present invention uses the residual waste plasma of Limulus reagent as raw material, salts out the protein through saturated ammonium sulfate solution, freezes and centrifuges to obtain Limulus hemocyanin, and then desalts the PBS solution of hemocyanin through MW3500Da dialysis bag, and passes through SephacrylS‑ 100 propylene dextran gel column purification, concentrated treatment to obtain high-efficiency phenoloxidase activity of hemocyanin, the reaction initial velocity Vi = 74.52 nmol · (min · mg). The product obtained by the present invention has high purity and high activity, and can be used to prepare efficient, convenient, and low-cost micro-detectors for phenolic pollutants; the preparation method of the present invention is simple, and adopts MW3500Da dialysis bags and SephacrylS-100 acryl-dextran gel The combination of columns can achieve the effects of desalination, separation, purification and concentration at the same time.