31 results about "Phenol oxidase activity" patented technology

The present invention relates to a composition for detecting an infinitesimal quantity of β-1,3-glucan, a preparation method thereof and a diagnostic kit detecting β-1,3-glucan. The composition of the present invention shows phenoloxidase activity by β-1,3-glucan in the presence of calcium ions. Using the composition of the present invention, a sample is gathered from a specimen, the composition of the present invention and calcium ions are added to the sample, and β-1,3-glucan is detected by measuring phenoloxidase activity.

The invention relates to the technical field of enzymatic activity, in particular to an apple polyphenol oxidase accelerative activator and application thereof. The apple polyphenol oxidase accelerative activator comprises a mixture of dodecyltrimethylammonium bromide or hexadecyl trimethyl ammonium bromide and a Gemini surfactant according to the weight ratio of 10-2,000: 1-4; and the Gemini surfactant is dibromo-bis(dimethyl dodecyl) pentyl diammonium or dibromo-bis(dimethyl hexadecyl) pentyl diammonium. The invention further provides application of the apple polyphenol oxidase accelerative activator in improvement of apple polyphenol oxidase activity. The apple polyphenol oxidase activity can be increased to be 240%, and the use amount level of the apple polyphenol oxidase accelerative activator is low.

The invention relates to a preparation method and application of a prawn non-specific immunity reinforcing agent oligopeptide. The preparation method comprises the following steps of: mincing pacific alaskapollack dogmeat by a meat mincer and then placing in an enzyme reactor to heat and sterilize; triturating and then heating to 90 DEG C to preserve the temperature; sterilizing for 20 minutes in a constant temperature water bath; adding water and protease preparations to mix; carrying out enzymolysis under the condition of optimized enzymolysis process parameters; then heating enzymolysis liquid so that protease is inactivated; roughly filtering fishbones and centrifugalizing the enzymolysis liquid to obtain a soluble protein oligopeptide; ultrafiltering, purifying, freezing and drying and then obtaining protein oligopeptide powder. The protease preparations comprise Alcalase and Flavourzyme, and the enzymolysis process parameters comprise the enzymolysis temperature of 55 DEG C, the initial pH value of 8.5, the material and water ratio of 1:1 and the enzymolysis time of 3 hours. A macromolecule substance is intercepted by using a method for combining 8,000rpm high-speed centrifugalization and 100kDa ultrafiltration membrane ultrafiltrating purification, and 30 percent of protein oligopeptide with the molecular weight of smaller than 100Da is obtained. The oligopeptide powder replaces 5 percent of fish meal in prawn feed and can obviously improve the blood corpuscle density, the phenoloxidase activity, the respiratory burst activity, the SOD activity, the NOS activity and the specific growing rate of a prawn.

The invention discloses a processing method of a sulfur-free konjak slice. The processing method comprises the following steps of (1) washing the konjak; (2) rubbing the peel of the konjak bulb; (3) slicing and collecting the konjak bulb; (4) blanching the konjak slice by steam, deactivating enzyme, and inhibiting the activity of polyphenol oxidase; (5) treating the konjak slice by konjak powder,so as to coat one layer of konjak powder onto the surface of the konjak slice; (6) drying the konjak slice by microwaves. The processing method of the sulfur-free konjak slice has the advantages thatthe fresh konjak is used as the raw material; the special processing method is adopted, so as to obtain the sulfur-free konjak slice.

The invention discloses a preparation method of a phenoloxidase active protein. The preparation method comprises the following steps: salting out proteins from a raw material waste blood plasma residual on Tachypleus Amebocyte Lysate by a saturated ammonium sulfate solution, performing refrigerating centrifugation to obtain limulus hemocyanin, performing dialysis desalting on a PBS solution of thehemocyanin through an MW 3500 Da dialysis bag, and carrying out Sephacryl S-100 allyl dextran gel column purification and concentrating treatment to obtain high-efficiency phenoloxidase active hemocyanin, wherein the initial reaction rate Vi of the high-efficiency phenoloxidase active hemocyanin is 74.52 nmol.(min.mg). The product obtained in the invention has a high purity and a high activity, and can be used for preparing a phenolic pollutant micro-detector with the advantages of high efficiency, simplicity and low cost; and the preparation method is simple, and simultaneously achieves desalting, separating, purifying and concentrating effects by cooperating the MW 3500 Da dialysis bag with the Sephacryl S-100 allyl dextran gel column.

The invention discloses a method for brewing cherry fruit wine. The method is characterized by comprising the following steps of: after selecting, washing and stoning cherries serving as raw materials, obtaining primary pulp by juicing; adding the following substances such as pectinase, calcium citrate, vitamin C, and g / L kojic acid into the primary pulp; standing for 5 to 24 hours; preparing clear juice by performing centrifugal separation; inoculating 0.05 to 2 percent of fruit wine yeast based on the weight of the clear juice; controlling a temperature and fermenting, wherein the primary fermentation temperature is between 16 and 28 DEG C, the fermentation time is 7 to 15 days, the secondary fermentation temperature is between 15 and 20 DEG C, and the fermentation time is 20 to 40 days; aging for 3 to 6 months; clarifying and filtering by adopting an ultra-filtration method; and filling, sterilizing and packaging to obtain finished cherry fruit wine. The method effectively improvesa production process of cherry wine, reduces the content of polyphenol fundamentally, inhibits the activity of polyphenol oxidase, reduces harsh taste and improves stability. Compared with the prior art, the cherry fruit wine produced by the method maintains effective nutrient components, reduces the harsh taste and has a pure flavor.

The present invention discloses a shrimp meat separation device using a vibration and separation method and belongs to the technical field of shrimp meat processing. The device comprises a box body and a vibration screen arranged inside the box body; a clamping block is connected with one side of the vibration screen; a sifting spring is connected with the other side of the vibration screen; a material suction pipe is connected with the inner wall of the box body at the lower end of the vibration screen; the material suction pipe is connected with a water pump through a support pipe; the waterpump is connected with a material outlet pipe; connection springs are connected with the outer wall of the head end of the material suction pipe; and the connection springs are connected with a movable cover distributed with cover holes. The device is high in working reliability, rapid in shrimp meat separation speed and good in effects, enables the shrimp meat to be compact in texture, inhibitsshrimp polyphenol oxidase activity in the shrimp meat body, effectively extends shelf life of the shrimp meat and improves mouthfeel of the shrimp meat.

The invention discloses a method and specific primers of detecting the character of the activity of wheat polyphenol oxidase. The primers provided by the invention for detecting the character of the activity of wheat polyphenol oxidase is a pair of primers consisting of the nucleotide sequences of the sequence 1 and the sequence 2 in the sequence table. The method of detecting the character of the activity of wheat polyphenol oxidase is that PCR amplification can be conducted by taking the DNA of a to-be-detected gene group as a templet and taking the nucleotide sequences of the sequence 1 and the sequence 2 in the sequence table as primers; and then the amplified outcomes can be detected to see whether 750 to 1000 bp of bands exist. The method and specific primers can play an important role both in the detection of the character of the activity of wheat polyphenol oxidase and in the breeding.

The invention relates to the technical field of enzyme activity, in particular to an apple polyphenol oxidase activity promoter and application thereof. The apple polyphenol oxidase activity promoter of the present invention comprises lauryltrimethylammonium bromide or hexadecyltrimethylammonium bromide and Gemini surfactant in a weight ratio of 10~2000:1~4 The mixture that mixes in proportion, described Gemini surfactant is dibromide-bis(dimethyldodecyl)pentammonium or dibromide-bis(dimethylhexadecyl)pentammonium. The present invention also includes the application of the above accelerator to improve the activity of apple polyphenol oxidase, which can increase the activity of apple polyphenol oxidase to 240%, and the consumption level of the accelerator is low.

The invention discloses a processing method of sulfur-free konjac chips, which comprises the following steps: 1) washing the konjac; 2) rubbing the konjac corms; 3) slicing and collecting the konjac corms; 4) 1. Use steam to blanch the konjac chips to inactivate enzymes to inhibit the activity of polyphenol oxidase; 5) process the konjac chips with konjac flour so that the surface of the konjac chips is coated with a layer of konjac flour; 6) konjac chips Carrying out microwave drying The object of the present invention is to provide a kind of processing method of sulfur-free konjac slices, use fresh konjac as raw material, through specific processing method, obtain sulfur-free konjac slices.

The invention relates to a preparation method and application of a prawn non-specific immunity reinforcing agent oligopeptide. The preparation method comprises the following steps of: mincing pacific alaskapollack dogmeat by a meat mincer and then placing in an enzyme reactor to heat and sterilize; triturating and then heating to 90 DEG C to preserve the temperature; sterilizing for 20 minutes in a constant temperature water bath; adding water and protease preparations to mix; carrying out enzymolysis under the condition of optimized enzymolysis process parameters; then heating enzymolysis liquid so that protease is inactivated; roughly filtering fishbones and centrifugalizing the enzymolysis liquid to obtain a soluble protein oligopeptide; ultrafiltering, purifying, freezing and drying and then obtaining protein oligopeptide powder. The protease preparations comprise Alcalase and Flavourzyme, and the enzymolysis process parameters comprise the enzymolysis temperature of 55 DEG C, the initial pH value of 8.5, the material and water ratio of 1:1 and the enzymolysis time of 3 hours. A macromolecule substance is intercepted by using a method for combining 8,000rpm high-speed centrifugalization and 100kDa ultrafiltration membrane ultrafiltrating purification, and 30 percent of protein oligopeptide with the molecular weight of smaller than 100Da is obtained, the further filteration is carried out by 1kDa / 5kDa ultrafiltration membrane to obtain an ultrafiltrate, and the oligopeptide powder is obtained by freezing and drying the ultrafiltrate. The oligopeptide powder replaces 5 percent of fish meal in prawn feed and can obviously improve the blood corpuscle density, the phenoloxidase activity, the respiratory burst activity, the SOD activity, the NOS activity and the specific growing rate of a prawn.

The invention discloses a preparation method of phenol oxidase active protein. The present invention uses the residual waste plasma of Limulus reagent as raw material, salts out the protein through saturated ammonium sulfate solution, freezes and centrifuges to obtain Limulus hemocyanin, and then desalts the PBS solution of hemocyanin through MW3500Da dialysis bag, and passes through SephacrylS‑ 100 propylene dextran gel column purification, concentrated treatment to obtain high-efficiency phenoloxidase activity of hemocyanin, the reaction initial velocity Vi = 74.52 nmol · (min · mg). The product obtained by the present invention has high purity and high activity, and can be used to prepare efficient, convenient, and low-cost micro-detectors for phenolic pollutants; the preparation method of the present invention is simple, and adopts MW3500Da dialysis bags and SephacrylS-100 acryl-dextran gel The combination of columns can achieve the effects of desalination, separation, purification and concentration at the same time.

The invention provides a method for preparing a lotus root juice beverage, which belongs to the technical field of fruit and vegetable juice beverage processing. The invention uses the whole lotus root as a raw material, and prepares the lotus root juice beverage through the steps of cleaning, cutting, color protection, beating, vacuum microwave heating, two-stage ultrasonic-assisted compound enzymolysis, centrifugation and filtration. The invention simplifies the cumbersome process of peeling and removing knots in the processing of lotus root juice, reduces the generation of processing waste, and increases the polyphenol content in the lotus root juice; vacuum microwave heating is carried out on the lotus root pulp to rapidly passivate the oxidation of polyphenols Enzyme activity, reducing the loss of polyphenols oxidation; ultrasonic-assisted composite enzymatic hydrolysis technology promotes the dissolution of polyphenols, and improves the antioxidant activity of lotus root juice. At the same time, the pretreatment time is shortened, the polyphenolic components of the lotus root juice beverage are retained to the greatest extent, its antioxidant activity is significantly improved, and the product quality is improved.