Polyphenol oxidase sulfur-free inhibitor for bulbus fritillariae thunbergii as well as formula and preparation method of polyphenol oxidase sulfur-free inhibitor

A technology of polyphenol oxidase and fritillary fritillary, applied in the field of traditional Chinese medicine processing, can solve problems such as unreasonable overall design, avoid exogenous toxin sulfur and its compounds, avoid changes in the content of active ingredients, prevent browning changing effect

Inactive Publication Date: 2017-02-22
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the above solution solves the deficiencies of the prior art to a certain extent, the polyphen...

Method used

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  • Polyphenol oxidase sulfur-free inhibitor for bulbus fritillariae thunbergii as well as formula and preparation method of polyphenol oxidase sulfur-free inhibitor
  • Polyphenol oxidase sulfur-free inhibitor for bulbus fritillariae thunbergii as well as formula and preparation method of polyphenol oxidase sulfur-free inhibitor
  • Polyphenol oxidase sulfur-free inhibitor for bulbus fritillariae thunbergii as well as formula and preparation method of polyphenol oxidase sulfur-free inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The formula of the non-sulfur inhibitor of fritillaria polyphenol oxidase includes 1-5 parts of vitamin C, 8-12 parts of sodium chloride and 10-14 parts of citric acid. The formula is used to make the sulfur-free inhibitor of fritillary fritillary polyphenol oxidase. The polyphenol oxidase activity (OD) was used as an indicator of the inhibitory effect. Wherein, the assay method of PPO activity of Fritillaria japonicus is as follows:

[0026] (1) Prepare the following materials required for the determination of PPO activity of Fritillaria japonicus.

[0027] 1. Test materials

[0028] Fresh Fritillaria thunbergii Miq. was purchased from Pan'an Fritillaria thunbergii Planting Base in Zhejiang Province. It was identified as the bulb of Fritillaria thunbergii Miq. by Professor Xiong Yaokang from Zhejiang University of Traditional Chinese Medicine.

[0029] 2. Main reagents

[0030] Ascorbic acid (vitamin C) (analytically pure) (batch number: 20111105, Guangdong Guanghu...

Embodiment 2

[0055] The formula of this Zhejiang Fritillaria polyphenol oxidase sulfur-free inhibitor includes 1-5 parts of vitamin C, 8-12 parts of sodium chloride, 10-14 parts of citric acid, 5-9 parts of lycopene, and 10- 12 servings. The formula is used to make the sulfur-free inhibitor of fritillary fritillary polyphenol oxidase. The same method for measuring the PPO activity of Fritillaria japonicus as in Example 1 was used to determine the inhibitory effect of the sulfur-free inhibitor of Fritillaria japonicus polyphenol oxidase in this example. Polyphenol oxidase activity (OD) was used as an index of inhibitory effect. The results are shown in Table 1-2.

[0056] Table 1-2 Test results

[0057]

Embodiment 3

[0059] The formula of the sulfur-free inhibitor of fritillary fritillaria polyphenol oxidase includes 1 part of vitamin C, 8 parts of sodium chloride, 10 parts of citric acid, 5 parts of lycopene and 10 parts of β-carotene. The formula is used to make the sulfur-free inhibitor of fritillary fritillary polyphenol oxidase. The same method for measuring the PPO activity of Fritillaria japonicus as in Example 1 was used to determine the inhibitory effect of the sulfur-free inhibitor of Fritillaria japonicus polyphenol oxidase in this example. Polyphenol oxidase activity (OD) was used as an index of inhibitory effect. The results are shown in Table 1-3.

[0060] Table 1-3 Test results

[0061]

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Abstract

The invention belongs to the technical field of traditional Chinese medicine processing, and in particular relates to a polyphenol oxidase sulfur-free inhibitor for bulbus fritillariae thunbergii as well as a formula and a preparation method of the polyphenol oxidase sulfur-free inhibitor. The inhibitor is prepared from the following optimized components in parts by weight: 1-5 parts of vitamin C, 8-12 parts of sodium chloride, and 10-14 parts of citric acid. The polyphenol oxidase sulfur-free inhibitor has the advantages that the activity of polyphenol oxidase is inhibited by the main enzyme inhibition components including vitamin C, sodium chloride and citric acid, the activity of PPO (polyphenol oxidase) is inhibited, so that the browning of the raw medicinal material can be prevented, then the appearance quality is not influenced, therefore, the effect of preventing the appearance quality decrease of bulbus fritillariae thunbergii is achieved, meanwhile, the real sulfur-free processing of the overall process is realized, and the integral design is reasonable.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine processing, and in particular relates to a sulfur-free inhibitor of fritillary fritillaria polyphenol oxidase and its formula and preparation method. Background technique [0002] Sulfur fumigation is a traditional processing method for the processing and maintenance of Chinese medicinal materials. This method uses sulfur dioxide gas generated by sulfur combustion to smoke medicinal materials and decoction pieces. The antiseptic function is beneficial to the storage of Chinese herbal medicines and decoction pieces. Many Chinese herbal medicines, such as Fritillaria fritillaria, Chinese yam, kudzu root, Gastrodia elata, wolfberry, etc., in order to achieve a good appearance and prolong storage time in their production areas, when the medicinal materials are harvested and processed, the medicinal farmers often use the sulfur fumigation method for treatment, and even more Second...

Claims

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Application Information

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IPC IPC(8): A61K36/8966A61K47/22A61K47/12A61K47/02A61K47/06A61K47/46
CPCA61K36/8966A61K47/02A61K47/06A61K47/12A61K47/22A61K47/46A61K2236/10
Inventor 熊耀康张春椿张水利俞冰李石清张婷李效贤
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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