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32results about How to "Inhibits polyphenol oxidase activity" patented technology

Brown inhibitor for cutted fresh vegetable and its application

A browning inhibitor for the cut fresh vegetable is prepared from cysteine, acetic acid, citric acid, calcium chloride and water. It is applied through washing and cutting fresh vegetable, immersing the solution of said browning inhibitor for 8-15 min, packing and storing at 4-5 deg.C for more than 12 days.
Owner:NANJING AGRICULTURAL UNIVERSITY

Method for preprocessing Asiatic wormwood to preserve color in combination with quick-freezing and unfreezing for keeping quality

The method for preprocessing Asiatic wormwood to preserve the color in combination with quick-freezing and unfreezing for keeping quality belongs to the technical field of fruit-vegetable food storage and processing. The process of the method is as follows: Asiatic wormwood with tender leaves and stalks is chosen, washed and then added with compound enzyme inhibitor, and by regulating the components, content and material-to-solution ratio of the enzyme inhibitor, the activity of polyphenol oxidase in the Asiatic wormwood is inhibited; the Asiatic wormwood is then blanched in boiled water, thepH value of the solution is regulated, the blanching time is adjusted, and while substances with bed flavor, such as absinthin, are completely removed, the loss of the Asiatic wormwood pigment is reduced; after cooling, metal ion color-preserving processing is carried out, and the dosage of metal ions and the pH value of color preservative are determined; after being sufficiently blanched, the Asiatic wormwood is pulped and filtered, vacuum packages are adopted for precooling and medium-frequency ultrasonic quick-freezing, and the Asiatic wormwood is refrigerated under the condition of negative 18 DEG C, and is ultrasonically unfrozen when being applied later. High-quality Asiatic wormwood product is produced by the method, the method is economic and practical, the cost investment is low,the process is simple and easy to implement, and the method can provide technical support for the industrialized production of Asiatic wormwood.
Owner:JIANGNAN UNIV +1

Mulberry active phenolic compound and pollution-free extraction and purification method thereof

The invention discloses a mulberry active phenolic compound and a pollution-free extraction and purification method thereof. The method comprises the steps that microwave and vapor high temperature instantaneous enzyme deactivation is carried out on fresh mulberries in sequence, then mechanical crushing, centrifugal layering, food grade acidified alcohol extraction, vacuum concentration and ultrafiltration membrane purification are carried out, and therefore a mulberry phenolic compound extracting solution with the biological activity is obtained. An organic reagent with the low concentration is adopted, mulberry crushing and phenolic compound extraction and purification are achieved under the mild condition, and the natural character and biological activity of the mulberry phenolic compound can be maintained. Compared with a conventional extraction method, the mulberry extracting solution has the higher-concentration total phenol and total anthocyanins, the extraction solution has the good color and luster and high oxidation resistance, alpha-glucosidase enzymatic inhibitory activity and food safety, and the mulberry active phenolic compound can be used as a safety raw material of antioxidant and hypoglycemic drug and health care product development. The conditions are mild, operation is convenient, and industrial production of high-activity natural products is facilitated.
Owner:广东普莱健康食品有限公司

Method for preserving cut apple for ultra-long time by conditioning air

The invention relates to a method for preserving cut apple for an ultra-long time by conditioning air. The method comprises the following steps: (1) cutting complete apples into blocks; (2) protecting colour of the apple blocks by using a colour protecting agent and coating films on the apple blocks by using a film coating agent; (3) draining the apple blocks and then processing the apple blocks in a continuous high-oxygen-concentration condition; and (4) carrying out gas packing the processed apple blocks, sterilizing the apple blocks and finally refrigerating the apple blocks, wherein all the operations are carried out at 0-5 DEG C. According to the method, the apples are cut at a low temperature and then processed under a high-oxygen-concentration condition; compared with the process of only refrigerating cut apples, the method provided by the invention has the advantages that the preservation period of the cut apple can be prolonged much longer, and due to the high oxygen concentration, the growth of some bacterium and funguses can be suppressed, the rottenness of the fruits and vegetables in preservation can be reduced, the respiration effect and ethylene synthesis of the fruits and vegetables can be reduced, the tissue brown stain can be slowed and the generation of substances with peculiar smells such as acetaldehyde and ethanol and the like can be reduced, so that the quality of the fruits and vegetables in preservation can be improved.
Owner:SDIC ZHONGLU FRUIT JUICE +1

Processing method of dehydrated lindera glauca chilli sauce

The invention provides a processing method of a dehydrated lindera glauca chilli sauce. The method comprises the following steps: (1) preparing a fermented chilli sauce; (2) preparing fresh lindera glauca raw material; (3) mixing and protecting color; (4) quick-freezing; (5) vacuum freeze-drying, namely quickly putting the quick-frozen lindera glauca chilli sauce in a vacuum freeze-drying apparatus, quickly reducing the vacuum degree in the apparatus to 50pa and starting heating drying on the lindera glauca chilli sauce until the moisture content of the raw sauce is lower than 3%, thereby obtaining the dehydrated lindera glauca chilli sauce. The processing method of the dehydrated lindera glauca chilli sauce is capable of solving the problems of inconvenient preservation, browning and the like of lindera glauca and chilli in the chilli sauce and overcoming the defects of drying the lindera glauca in the sun and by freezing; the prepared dehydrated chilli sauce and lindera glauca have original colors, fragrances and tastes, respectively, and great damage of nutritional ingredients is prevented, and the dehydrated lindera glauca chilli sauce is good in quality and up to the national related food sanitation quality standards; besides, the development of agricultural economy is promoted.
Owner:HUBEI TEMIAO FOOD CO LTD

Method for coating preservation of Litchi chinensis bacterium by phellinus igniarius polysaccharide

InactiveCN106578029AExtended shelf lifeReduce the metabolic intensity of breathingFruits/vegetable preservation by coatingEvaporationPhellinus igniarius
The invention discloses a method for coating preservation of Litchi chinensis bacterium by phellinus igniarius polysaccharide. The method comprises the following steps: classifying the harvested Litchi chinensis bacterium, picking the Litchi chinensis bacterium consistent in maturity and sizes and free of mechanical damage and plant diseases and insect pests, and soaking in a phellinus igniarius polysaccharide solution of which the concentration is 0.8 to 1.2% for 2 to 6min, carrying out air drying by cold air until the surfaces of the Litchi chinensis bacterium are dried and formed by films and then performing preservation at the temperature of 4 DEG C. The invention further discloses a preservation method of the Litchi chinensis bacterium by fumigating the phellinus igniarius polysaccharide solution on the surfaces of the Litchi chinensis bacterium, and further discloses a method for extracting the phellinus igniarius polysaccharide solution. In addition, the invention has the beneficial effects of lowering the respiration rate of the Litchi chinensis bacterium, inhibiting the activity of polyphenol oxidase of the Litchi chinensis bacterium, reducing internal moisture evaporation, delaying aging and having low cost and long preservation time; furthermore, the phellinus igniarius polysaccharide is high in nutritional values and has a variety of biological activities of oxidation resistance, immunity enhancement, tumor resistance and the like, and the films are coated on the surfaces of the Litchi chinensis bacterium, and free of any toxic and side effects to human bodies, thereby being natural and environment-friendly.
Owner:GUANGZHOU JUCHAN MODERN AGRI RES INST CO LTD

Method for extracting anthocyanin from blueberry

The invention discloses a method for extracting anthocyanin from blueberry. The method comprises the following steps of using aspergillus oryzae-fermented rice extract as an extracting additive to assist microwave treatment, extracting by ethyl acetate, and purifying by D101 macroporous resin; finally, eluting by ethyl alcohol, and freeze-drying, so as to prepare the anthocyanin powder of the blueberry. The method has the advantages that compared with the traditional technology, the content of anthocyanin in the prepared anthocyanin powder of the blueberry is increased by 10 to 14%; the technical foundation is provided for the production and processing of the anthocyanin.
Owner:广西南宁桂尔创环保科技有限公司

Pleurotus nebrodensis preservation method

The invention relates to the technical field of fruit and vegetable preservation and in particular relates to a pleurotus nebrodensis preservation method. The method comprises the following steps: performing reduced pressure treatment on the harvested fresh pleurotus nebrodensis, and fumigating the pleurotus nebrodensis by adopting plant essential oil; filling nitrogen, and performing sealed treatment, and finally, taking out the pleurotus nebrodensis, packaging by adopting a preservative film, binding the bag opening, and storing under the condition of 0-1 DEG C until the storage period is ended. According to the method disclosed by the invention, the possibility of rot can be reduced due to use of the plant essential oil, the commodity rate is improved, and the plant essential oil comes from the essential oil extracted from natural plants and has incomparable safety advantage which cannot be achieved by chemically synthetic preservatives.
Owner:天津市蓟县中亿建良食用菌种植专业合作社

Blueberry jelly and preparation method thereof

The invention discloses a blueberry jelly and a preparation method thereof. The blueberry jelly is mainly composed of the following raw materials in parts by weight: 40-60 parts of blueberry anthocyanin extraction powder, 6-10 parts of calcium powder, 15-25 parts of seaweed powder, 5-15 parts of peppermint oil, 1-5 parts of fat sea powder, 1-5 parts of chrysanthemum powder, 1-5 parts of lychee juice, 50-70 parts of soybean protein solution, and appropriate amount of water. The blueberry jelly of the present invention has a sweet and sour taste, contains various health-care active ingredients, and has more obvious functions of protecting eyesight and refreshing eyesight. At the same time, various raw materials are nutritionally balanced and reasonably matched, and are rich in anthocyanins, flavonoids, vitamins and trace amounts of Elements and other health substances, the blueberry jelly has high nutritional content, is convenient to carry and has low cost.
Owner:广西南宁桂尔创环保科技有限公司

Preparation and using method of V.spinulosa Yan rhizome browning inhibitor

The invention relates to preparation and a using method of a V.spinulosa Yan rhizome browning inhibitor. In the preparation of the inhibitor, mixed solution of oxalic acid, citric acid and ascorbic acid is adopted. The using method of the V.spinulosa Yan rhizome browning inhibitor comprises the following steps that: after harvested V.spinulosa Yan rhizomes are cleaned to remove sludge, impurities and the rotten, mildewed and nigrescent V.spinulosa Yan rhizomes, the V.spinulosa Yan rhizomes are soaked in aqueous solution containing the inhibitor to be stored and can be kept being not discolored for 60 days; or before being processed, the V.spinulosa Yan rhizomes are soaked for 2 to 8 hours. The V.spinulosa Yan rhizomes browning inhibitor can be used for effectively inhibiting the V.spinulosa Yan rhizomes from being browned in the machining process and has the advantages of long time of inhibiting the V.spinulosa Yan rhizomes from being browned, good effect, no damage to the original flavor of V.spinulosa Yan, high safety, no toxic or side effects on a human body and the like.
Owner:ANHUI AGRICULTURAL UNIVERSITY

Method for shortening tobacco leaf baking time of closed barking room

The invention discloses a method for shortening the tobacco leaf baking time of a closed barking room, belonging to the technical field of modulation of flue-cured tobacco. The method comprises the following steps: manually introducing oxygen in the front yellowing stage, so as to maintain the oxygen concentration in the closed barking room at 0.5%-2.0%; manually introducing carbon dioxide from the middle-later yellowing stage to a color fixation stage, so as to maintain the carbon dioxide concentration in the closed barking room at 0.5%-2.0%; and continuing to introduce carbon dioxide from alater color fixation stage to a tendon drying stage, so as to maintain the carbon dioxide concentration in the closed barking room at 0.1%-0.8%. The method has the beneficial effects that the respiratory action of tobacco leaves in the baking process can be adjusted, the baking time can be shortened, the quality of the tobacco leave can be improved, the utilization rate of the baking room can be increased, the energy consumption cost can be lowered, and the increase of farmer's income can be promoted.
Owner:云南省烟草公司楚雄州公司 +1

Compound blueberry buccal tablets and preparation method thereof

The present invention discloses compound blueberry buccal tablets and a preparation method thereof. The compound blueberry buccal tablets are mainly composed of the following raw materials in the following parts by weight: 40-60 parts of blueberry anthocyanin extract powder, 6-10 parts of calcium powder, 15-25 parts of seaweed powder, 5-15 parts of peppermint oil, 1-5 parts of semen sterculiae lychnophorae powder, 1-5 parts of chrysanthemum powder, 1-5 parts of litchi juice, 50-70 parts of soybean protein solution, and a proper amount of water. The blueberry buccal tablets are sour, sweet andrefreshing in taste, contain a variety of health-care active ingredients, and have more obvious functions of protecting vision, refreshing mind and improving eyesight. At the same time, the various raw materials are balanced in nutrition and reasonable in matching, and rich in anthocyanins, flavonoids, vitamins, trace elements and other health-care substances. The blueberry buccal tablets producea large amount of carbon dioxide gas after entering water, and thus are quickly dissolved, rapid in drug effects, high in bioavailability, easy to carry, and low in cost.
Owner:广西南宁桂尔创环保科技有限公司

Kiwifruit NFC juice and preparation method thereof

The invention discloses a kiwifruit NFC juice and a preparation method thereof, and belongs to the technical field of food processing. The kiwifruit NFC juice is obtained by selecting, washing, soaking, color protection, pulping, adjusting the ratio of sugar to acid and adding natural antibacterial substance nisin in combination with the ultra-high pressure sterilization technology. The juice aimsto overcome the defects caused by the heat sterilization technology and retain the original fresh characteristics and nutritional characteristics of the kiwifruit NFC juice to the utmost extent. Themethod has the advantages of being simple and efficient, nutritious, healthy and low in production cost. The prepared kiwi NFC juice has taste, flavor and nutritional value closer to those of fresh fruit, and is rich in flesh and unique in flavor.
Owner:SHAANXI UNIV OF SCI & TECH

Noodles and production method thereof

The invention relates to noodles and a production method thereof. Particularly, the production method of the noodles disclosed by the invention comprises the step of fermenting flour through fermentedstarch glue, preferably, the method comprises the following steps of (1) performing pre-fermenting so as to obtain the fermented starch glue; and (2) performing official fermenting, wherein the pre-fermenting step comprises the substeps of fermenting the flour of which the weight is 5-55% of the total weight of the flour to be fermented, to obtain the fermented starch glue, then mixing the fermented starch glue with the surplus flour, and then performing official fermenting. According to the noodles and the production method thereof disclosed by the invention, based on a conventional processing technology of raw fresh noodles and fine dried noodles, a fermentation technology is adopted, a production technique for raw fresh noodles and quickly-cooked fine dried noodles, which is simple tooperate and low in cost, is provided, and the made raw fresh noodles and the made fine dried noodles are better in color and better in mouth feel.
Owner:WILMAR SHANGHAI BIOTECH RES & DEV CENT

High oxygen fresh-keeping method for fresh-cut apples

The invention relates to a high-oxygen fresh-keeping method of fresh-cut apples. The method comprises the following steps: (1) treating unbroken apples at a low temperature and under a continuous high-oxygen concentration condition; (2) taking out the apples and cutting the apples so as to obtain apple blocks; and (3) performing color-protecting and coating treatment on the apple blocks by a color-protecting agent and a coating agent, then performing air-filling packaging and sterilization on the apple blocks, and finally, refrigerating. According to the method, the unbroken apples are subjected to the continuous high-oxygen treatment at a low temperature and then are cut, and the growth of bacteria and fungi can be inhibited under the high-oxygen condition, so that the decomposition of fruits and vegetables during the storage is alleviated, the respiratory action and the ethylene synthesis of fruits and vegetables are relieved, the tissue browning of fruits and vegetables is slowed down, and substances with peculiar smells, such as acetaldehyde and ethanol, are decreased. Thus, the storage quality of fruits and vegetables is effectively improved. The effects of the method provided by the invention are obviously superior to the treatment effects of a method in which the refrigeration and the cutting of apples are separately performed.
Owner:SDIC ZHONGLU FRUIT JUICE +1

Ultra-Long-Term Modified Atmosphere Preservation Method for Sliced ​​Apples

The invention relates to a method for preserving cut apple for an ultra-long time by conditioning air. The method comprises the following steps: (1) cutting complete apples into blocks; (2) protecting colour of the apple blocks by using a colour protecting agent and coating films on the apple blocks by using a film coating agent; (3) draining the apple blocks and then processing the apple blocks in a continuous high-oxygen-concentration condition; and (4) carrying out gas packing the processed apple blocks, sterilizing the apple blocks and finally refrigerating the apple blocks, wherein all the operations are carried out at 0-5 DEG C. According to the method, the apples are cut at a low temperature and then processed under a high-oxygen-concentration condition; compared with the process of only refrigerating cut apples, the method provided by the invention has the advantages that the preservation period of the cut apple can be prolonged much longer, and due to the high oxygen concentration, the growth of some bacterium and funguses can be suppressed, the rottenness of the fruits and vegetables in preservation can be reduced, the respiration effect and ethylene synthesis of the fruits and vegetables can be reduced, the tissue brown stain can be slowed and the generation of substances with peculiar smells such as acetaldehyde and ethanol and the like can be reduced, so that the quality of the fruits and vegetables in preservation can be improved.
Owner:SDIC ZHONGLU FRUIT JUICE +1

Flue-cured tobacco blending coloring agent as well as preparation method and application thereof

The invention discloses a flue-cured tobacco blending coloring agent as well as a preparation method and a use method thereof. The flue-cured tobacco blending coloring agent consists of antioxidants,a synergist and a surfactant, and is used in a flue-cured tobacco blending coloring stage. The natural antioxidants vitamin C and vitamin E can effectively prevent miscellaneous colors generated by excessive oxidation of phenolic substances in the tobacco blending process. Citric acid serves as the synergist of the antioxidants, and the action effect of the antioxidants can be remarkably improved.Sodium dodecyl sulfate serves as the surfactant and can improve the permeability of a tobacco leaf tissue structure, and therefore, on one hand, the antioxidants and the synergist are promoted to enter tobacco leaf cell tissues to take effect, and on the other hand, water in the tobacco leaf cell tissues is promoted to be rapidly discharged; and excessive oxidation of phenolic substances which are not discharged in time in the tobacco blending process is prevented, and yellow is fixed.
Owner:HENAN AGRICULTURAL UNIVERSITY

Preparation method of fresh-keeping solution for grapes

The invention discloses a preparation method of a fresh-keeping solution for grapes. The method comprises the following steps: uniformly mixing, by weight, 10-15 parts of gelatin, 10-12 parts of potassium myristate and 8-10 parts of sucrose ester, dissolving the obtained mixture in 30-40 parts of water, sequentially adding 5-8 parts of Tagetes erecta flower extract, 2-4 parts of clove oil, 1-3 parts of vitamin C and 6-9 parts of modified soybean proteins, and carrying out high-pressure homogenization to obtain the fresh-keeping solution for grapes. The fresh-keeping solution can significantlyprolong the storage period of the grapes, preserve the good flavor of the grapes and improve the benefits of enterprises.
Owner:李侠

Preparation method of fresh apple juice

The invention discloses a preparation method of fresh apple juice. The preparation method comprises the following steps: (1) harvesting and warehousing; (2) selecting fruit; (3) cleaning and dicing; (4) performing anti-browning treatment; (5) sterilizing; (6) performing air drying; (7) preparing juice; (8) performing secondary enzyme deactivation; (9) cooling; and (10) filling: filling the cooled apple juice into a special sterilized glass bottle to obtain the fresh apple juice, and storing at the ice temperature of-1 to -4 DEG C. According to the invention, additives such as essence, pigments and preservatives do not need to be added into the fresh apple juice prepared by the method, enzyme deactivation and sterilization are performed by adopting a pure physical method, the current green, additive-free, safe and pollution-free production concepts are met, and the produced product is non-toxic, green and healthy.
Owner:SDIC ZHONGLU FRUIT JUICE

Traditional Chinese medicine-containing chalk

The invention discloses a traditional Chinese medicine-containing chalk. The chalk is prepared from the following components in parts by mass: 26-34 parts of kaolin, 15.7-23 parts of calcium carbonate, 6-14 parts of ammonium sulfate, 10-16 parts of licorice roots, 16-20 parts of platycodon grandiflorum, 11-16 parts of pericarpium citri reticulatae, 10-17 parts of semen raphanin, 7-11 parts of calcium sulfate, 6-10 parts of soluble starch, 8-12 parts of a flavoring agent and 21-26 parts of water. The chalk has the effect similar to those of medicinal chalk but does have the side effect of medicinal ingredients since traditional Chinese medicinal materials and volatile oil ingredients are added based on existing conventional chalks.
Owner:广安市广安区北仓路小学校

Preservative for litchis

The invention discloses a preservative for litchis. The preservative comprises, by weight, 8-14 parts of lignin, 5-12 parts of Arabic gum, 2-7 parts of citric acid, 7-11 parts of modified barley, 3-9parts of rheum officinale extract, 1-5 parts of cinnamon essential oil, 2-5 parts of chitosan, 1-3 parts of tartaric acid and 2-5 parts of tea oil. The preservative can significantly prolong the storage period of the litchis, preserve the good flavor of the litchis and improve the benefits of enterprises.
Owner:李侠

A kind of processing method of dehydrated mountain pepper chili sauce

The invention provides a processing method of a dehydrated lindera glauca chilli sauce. The method comprises the following steps: (1) preparing a fermented chilli sauce; (2) preparing fresh lindera glauca raw material; (3) mixing and protecting color; (4) quick-freezing; (5) vacuum freeze-drying, namely quickly putting the quick-frozen lindera glauca chilli sauce in a vacuum freeze-drying apparatus, quickly reducing the vacuum degree in the apparatus to 50pa and starting heating drying on the lindera glauca chilli sauce until the moisture content of the raw sauce is lower than 3%, thereby obtaining the dehydrated lindera glauca chilli sauce. The processing method of the dehydrated lindera glauca chilli sauce is capable of solving the problems of inconvenient preservation, browning and the like of lindera glauca and chilli in the chilli sauce and overcoming the defects of drying the lindera glauca in the sun and by freezing; the prepared dehydrated chilli sauce and lindera glauca have original colors, fragrances and tastes, respectively, and great damage of nutritional ingredients is prevented, and the dehydrated lindera glauca chilli sauce is good in quality and up to the national related food sanitation quality standards; besides, the development of agricultural economy is promoted.
Owner:HUBEI TEMIAO FOOD CO LTD

Antibacterial moisturizing degradable porous gel preservative film and preparation method thereof

The invention discloses an antibacterial moisturizing degradable porous gel preservative film and a preparation method thereof. The preparation method comprises the following steps: (1) putting a methacrylic acid monomer into deionized water, and adding double-long-chain methyl tertiary amine and cuprous bromide to obtain a component A solution; (2) dispersing ferulic acid in an ethanol solution, and adding 3, 4-vinyl dioxythiophene to obtain a component B solution; (3) dissolving chitosan in an acetic acid solution, and adding the component A solution, the component B solution and chloroethanol to obtain preservative film slurry; (4) heating the preservative film slurry, and adding L-cysteine to obtain porous gel preservative film slurry; and (5) preparing the porous gel preservative film. The porous gel preservative film prepared by the method is simple in principle, safe in components and good in antibacterial effect, solves the problem of poor mechanical property of a preservative film which takes Kottaki as a main material, is high in degradation rate, safe and environment-friendly, can realize industrial production, and has relatively high market popularization value.
Owner:侯龙辉

Fresh-cut fruit and vegetable preservation method adopting honey and plasma combined treatment

The invention discloses a fresh-keeping method for fresh-cut fruits and vegetables through honey and plasma combined treatment, and belongs to the technical field of fresh-cut fruit and vegetable storage and fresh-keeping. Soaking the fresh-cut fruits and vegetables in the honey solution, and after soaking is finished, taking out and draining off the fresh-cut fruits and vegetables; the drained fresh-cut fruits and vegetables are exposed to plasma for treatment; and packaging the fresh-cut fruits and vegetables subjected to plasma treatment, and refrigerating and storing. The fresh-keeping method provided by the invention effectively solves the problem that the fresh-cut fruits and vegetables are easy to discolor, prolongs the shelf life of the fresh-cut fruits and vegetables, retains the specific nutritional value of the fruits and vegetables, and is green, safe, simple and easy to implement.
Owner:JIANGNAN UNIV

Powder chalk

The invention discloses a medicated powder chalk. The composition of the medicated powder chalk comprises: 26-34 parts by mass of Guanyin soil, 16-23 parts by mass of borax, 6-14 parts by mass of ammonium chloride, 10-16 parts by mass of licorice, and 16-23 parts by mass of bellflower 20 parts by mass, 11-16 parts by mass of tangerine peel, 10-17 parts by mass of radish seeds, 7-11 parts by mass of food coloring, 6-10 parts by mass of soluble starch, 8-12 parts by mass of flavoring agent, and 21-26 parts by mass of water.
Owner:四川省广安中学实验学校

Method for extracting total ribonucleic acid from plants with polysaccharide and polyphenol by using silica membrane

ActiveCN102533737BImprove bindingInhibit RNase activityDNA preparationFiltrationSilica gel
The invention relates to a method for extracting total ribonucleic acid from plants with polysaccharide and polyphenol by using a silica membrane, and belongs to the field of nucleic acid purification. The method comprises the following steps of: adding a lysis solution for the plants with polysaccharide and polyphenol into a plant material with polysaccharide and polyphenol, which is ground by liquid nitrogen, performing centrifugal separation on the mixture, taking the supernate obtained through centrifugal separation out, transferring into a filtration column for filtration, adding absolute ethanol into filtrate, uniformly mixing, transferring into a silica membrane adsorbing column for adsorbing centrifugation, adding a protein removing solution and a deoxyribonuclease solution for protein removal centrifugation twice, adding a rinsing solution for desalting centrifugation, performing drying centrifugation, and adding deoxyribonuclease water for rinsing centrifugation to obtain a ribonucleic acid solution. The method has the characteristics of high efficiency, quickness and conciseness; and the purified ribonucleic acid (RNA) can be used for various downstream experiments suchas microarray analysis, in vitro translation, molecular cloning and the like.
Owner:TIANGEN BIOTECH BEIJING

A kind of mulberry active phenolic compound and its green extraction and purification method

The invention discloses a mulberry active phenolic compound and a pollution-free extraction and purification method thereof. The method comprises the steps that microwave and vapor high temperature instantaneous enzyme deactivation is carried out on fresh mulberries in sequence, then mechanical crushing, centrifugal layering, food grade acidified alcohol extraction, vacuum concentration and ultrafiltration membrane purification are carried out, and therefore a mulberry phenolic compound extracting solution with the biological activity is obtained. An organic reagent with the low concentration is adopted, mulberry crushing and phenolic compound extraction and purification are achieved under the mild condition, and the natural character and biological activity of the mulberry phenolic compound can be maintained. Compared with a conventional extraction method, the mulberry extracting solution has the higher-concentration total phenol and total anthocyanins, the extraction solution has the good color and luster and high oxidation resistance, alpha-glucosidase enzymatic inhibitory activity and food safety, and the mulberry active phenolic compound can be used as a safety raw material of antioxidant and hypoglycemic drug and health care product development. The conditions are mild, operation is convenient, and industrial production of high-activity natural products is facilitated.
Owner:广东普莱健康食品有限公司

A kind of fresh-keeping and color-protecting method of cattail cabbage

The invention discloses a method for preserving freshness and color of cattail cabbage. It specifically includes the following steps: (1) soaking the fresh cattail in low-temperature ozone water for a period of time; (2) removing the cattail obtained in the above steps and then putting it into a mixed solution of edible salt solution and edible calcium chloride at a certain temperature Soak for a period of time, take it out, and then place it in a sterile environment to drain; (3) treat the cattail catkins drained in step (2) with short-wave ultraviolet rays, and seal the package; (4) pack the cattail catkins obtained in step (3) The vegetables are stored in a low temperature environment. The crisp and fresh preservation method of the present invention does not form frozen bodies, produce ice crystals, or damage cell walls when frozen aquatic vegetables are preserved; therefore, the liquid juice does not lose and can be recovered after rehydration; the color will not change after 12 months of frozen preservation , after thawing and desalting to cook the cattail, the taste is crisp and tender as before; the process is simple, the ingredients are safe, the economic cost is low, it is beneficial to the large-scale fresh-keeping production of the cattail, and the practical value is high.
Owner:淮安康得乐食品有限公司
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