Method for coating preservation of Litchi chinensis bacterium by phellinus igniarius polysaccharide
A technology of coating fresh-keeping, Phellinus linteus polysaccharide, applied in the direction of protecting fruits/vegetables with a coating protective layer, can solve aging, withering, discoloration, deterioration and odor, loss of color, aroma, taste, shape and moisture, reduce Nutritional value and other issues, to achieve the effect of inhibiting the activity of polyphenol oxidase, reducing the level of metabolism, and enhancing immunity
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Embodiment 1
[0021] Take Phellinus linteus fruiting bodies, crush, extract with 5 times volume of 95% ethanol, defatted overnight, filter, extract residues with 10 times boiling water for 3 hours, collect the filtrate, extract twice, combine the filtrate, concentrate, centrifuge to remove the precipitate . The filtrate was slowly added with 75% ethanol under stirring until the ethanol concentration reached 25%, and the mixture was allowed to stand overnight and centrifuged. The supernatant was continuously added with 75% ethanol until the ethanol concentration reached 50%, allowed to stand overnight, and centrifuged to obtain crude polysaccharides.
Embodiment 2
[0023] Take Phellinus igniarius fruiting bodies, pulverize, extract with 6 times volume of 95% ethanol, defatted overnight, filter, extract the extract residue with 13 times boiling water for 5 hours, collect the filtrate, extract 4 times, combine the filtrate, concentrate, centrifuge, and remove the precipitate . The filtrate was slowly added with 75% ethanol under stirring until the ethanol concentration reached 25%, and the mixture was allowed to stand overnight and centrifuged. The supernatant was continuously added with 75% ethanol until the ethanol concentration reached 50%, allowed to stand overnight, and centrifuged to obtain crude polysaccharides.
Embodiment 3
[0025] In one embodiment, the method for extracting the Phellinus igniarius polysaccharide solution in the method is: taking the Phellinus igniarius fruiting bodies, crushing, leaching with 7 times the volume of 95% ethanol, overnight degreasing, filtering, and extracting the leaching residue with 11.5 times boiling water 4h, collect the filtrate, extract 3 times, combine the filtrate, concentrate, centrifuge, and remove the precipitate. The filtrate was slowly added with 75% ethanol under stirring until the ethanol concentration reached 25%, and the mixture was allowed to stand overnight and centrifuged. The supernatant was continuously added with 75% ethanol until the ethanol concentration reached 50%, allowed to stand overnight, and centrifuged to obtain crude polysaccharides.
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