Application of eremothecium in inhibition of polyphenol oxidase activity

A technology of polyphenol oxidase and Pseudocystis, which is applied in the field of polyphenol oxidase activity research, can solve the problems of unclear mechanism of action of natural anti-browning agents, uncertain function of natural anti-browning agents, etc., and achieve inhibition methods Multiple, high inhibition rate, wide application range

Pending Publication Date: 2020-07-31
LANZHOU UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the mechanism of action of natural anti-browning agents is not yet clear, and it is not sure which specific ingredients in natural anti-browning agents work

Method used

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  • Application of eremothecium in inhibition of polyphenol oxidase activity
  • Application of eremothecium in inhibition of polyphenol oxidase activity
  • Application of eremothecium in inhibition of polyphenol oxidase activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] experiment material

[0026] Potatoes: provided by Dingxi Academy of Agricultural Sciences, Gansu Province.

[0027] Main test reagent

[0028] Bovine serum albumin (biochemical reagent) Sigma company;

[0029] Coomassie brilliant blue G-250 (biochemical reagent) Sigma company;

[0030] Glucose (biochemical reagent) Sigma company;

[0031] Acetone, catechol, and ammonium sulfate were all of analytical grade and were purchased from Tianjin No. 3 Chemical Reagent Factory.

[0032] Test Instruments

[0033] Electric constant temperature water bath HH-S4, Beijing Kewei Yongxin Instrument Co., Ltd.;

[0034] Electronic analytical balance AB104-N, Mettler-Toledo Instrument Co., Ltd.;

[0035] UV-Vis Spectrophotometer UV-9200, American Varian Company;

[0036] Circulating water multi-purpose vacuum pump SHB-Ⅲ, Zhengzhou Great Wall Technology Industry and Trade Co., Ltd.;

[0037] Centrifuge L550, Changsha Xiangyi Centrifuge Co., Ltd.;

[0038] Refrigerated centrifuge ...

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Abstract

The invention provides an application of eremothecium in inhibition of polyphenol oxidase activity and belongs to the technical field of polyphenol oxidase activity research. In the application, eremothecium suspension liquid in the logarithmic phase can remarkably inhibit polyphenol oxidase, and the inhibition rate can reach 32%. The inhibition of the polyphenol oxidase by the eremothecium belongs to a mixed inhibition type, the eremothecium can react with not only the free polyphenol oxidase but also a complex of the polyphenol oxidase combined with a substrate, the inhibition ways are multiple, the inhibition rate is high, and the application range is wide.

Description

technical field [0001] The invention belongs to the technical field of research on polyphenol oxidase activity, and in particular relates to the application of Pseudocystis in inhibiting polyphenol oxidase activity. Background technique [0002] Polyphenol oxidase (PPO) is a kind of metalloprotease widely distributed in nature, which is ubiquitously present in the plastids of plants, fungi, and insects, and can even be detected on rotting plant residues in soil. active. Because of its convenient detection, it is one of the first few types of enzymes to be studied. [0003] Polyphenol oxidation (PPO) enzyme is the main cause of the discoloration of cut potatoes. During potato processing, the effective inhibition of enzymatic browning has always been the main factor affecting the development and utilization of potatoes. Browning means that under aerobic conditions, phenolase in plants catalyzes phenolic substances to form quinones, and quinones are further oxidized and polym...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L5/41A23L29/00
CPCA23L5/41A23L29/065A23V2002/00A23V2200/048
Inventor 赵萍肖宇轩张子亮白雪莹刘裕慧
Owner LANZHOU UNIVERSITY OF TECHNOLOGY
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