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Apple polyphenol oxidase accelerative activator and application thereof

A technology of polyphenol oxidase and activity accelerator, which is applied in the field of enzyme activity, can solve the problems of excessive dosage and insufficient regulation of enzyme activity, and achieve the effects of less dosage, low critical micelle concentration and good solubilization

Active Publication Date: 2017-08-29
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

From the above data, it can be seen that the amount of traditional surfactants is relatively large, and the regulation effect on enzyme activity is not obvious enough.

Method used

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  • Apple polyphenol oxidase accelerative activator and application thereof
  • Apple polyphenol oxidase accelerative activator and application thereof
  • Apple polyphenol oxidase accelerative activator and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] 1. Pretreatment

[0054] Wash the Shandong Yantai Red Fuji apples in clean water, drain them, and place them in a -20°C freezer for 12 hours.

[0055] 2. Extraction of crude enzyme solution

[0056] Peel the frozen apple into slices, weigh 58.82g, add 100mL phosphate buffer solution (0.2M, pH=6.5, containing 0.25% (v / v) (250μL) TritonX-100 and 2% (w / w ) (3.38g)PVPP), the ratio of liquid to material is 1.7mL / g, mix and beat for 1min, filter with four layers of ice gauze, separate the filtrate, centrifuge the obtained filtrate at 10000rpm at 4°C for 10min, take the supernatant, and put it in Store in -20°C freezer for later use.

[0057] 3. Determination of polyphenol oxidase activity

[0058] Using catechol as substrate, dilute PPO crude extract 25 times and add accelerator to test PPO enzyme activity. In the test system, the concentration of catechol was 150 mM. The accelerator is a compound of DTAB and 16-5-16, and its concentrations in the test system are: 2.0 mM...

Embodiment 2

[0061] The accelerator is a compound of DTAB and 12-5-12, and its concentrations in the test system are: 2.0 mM for DTAB and 20 μM for 12-5-12. All the other are identical with embodiment 1.

[0062] Compared with no accelerator, the enzyme activity of PPO increased by 75% after the accelerator was added.

Embodiment 3

[0064] The accelerator is a compound of CTAB and 16-5-16, and its concentrations in the test system are: 0.3 mM for CTAB and 10 μM for 16-5-16. All the other are identical with embodiment 1.

[0065] Compared with no accelerator, the enzyme activity of PPO increased by 100% after the accelerator was added.

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Abstract

The invention relates to the technical field of enzymatic activity, in particular to an apple polyphenol oxidase accelerative activator and application thereof. The apple polyphenol oxidase accelerative activator comprises a mixture of dodecyltrimethylammonium bromide or hexadecyl trimethyl ammonium bromide and a Gemini surfactant according to the weight ratio of 10-2,000: 1-4; and the Gemini surfactant is dibromo-bis(dimethyl dodecyl) pentyl diammonium or dibromo-bis(dimethyl hexadecyl) pentyl diammonium. The invention further provides application of the apple polyphenol oxidase accelerative activator in improvement of apple polyphenol oxidase activity. The apple polyphenol oxidase activity can be increased to be 240%, and the use amount level of the apple polyphenol oxidase accelerative activator is low.

Description

technical field [0001] The invention belongs to the technical field of enzyme activity, and in particular relates to an apple polyphenol oxidase activity promoter and application thereof. Background technique [0002] Polyphenol oxidase is a kind of metalloprotease widely distributed in nature. It is ubiquitous in plants and can oxidize phenols or polyphenols to corresponding quinones. It is widely used in food, medicine and environmental protection industries, and research on its activity can better solve problems such as food storage, plant resistance to pathogens and viruses, and biodegradation of industrial wastewater containing phenolic organic substances. [0003] At present, most researchers study the purified polyphenol oxidase (PPO), including exploring the optimal activity conditions of the enzyme, the stimulation of the enzyme activity by surfactants (sodium dodecyl sulfate (SDS)), etc., in order to achieve the maximum Possible purpose of increasing enzyme activi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02
Inventor 郭霞郭晶晶张礼飞万舒雨
Owner YANGZHOU UNIV
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