33 results about "Penicillium funiculosum" patented technology

Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A) enzymes from Penicillium funiculosum demonstrate a high level of specific performance in comparison to other Cel7 family member enzymes when formulated with purified EIcd endoglucanase from A. cellulolyticus and tested on pretreated corn stover. This result is true of the purified native enzyme, as well as recombinantly expressed enzyme, for example, that enzyme expressed in a non-native Aspergillus host. In a specific example, the specific performance of the formulation using purified recombinant Cel7A from Penicillium funiculosum expressed in A. awamori is increased by more than 200% when compared to a formulation using purified Cel7A from Trichoderma reesei.

The present invention relates to novel micro-organism, Penicillium funiculosum, to new enzymes mixture obtained from it and nucleic acid sequences thereto.

The invention relates to the technical field of novel materials and in particular relates to an antibacterial anti-mold water drainage PVC (Polyvinyl Chloride) tube and a production method thereof. The antibacterial anti-mold water drainage PVC tube comprises an antibacterial anti-mold layer as an inner layer and a PVC composite material layer as an outer layer, wherein the antibacterial anti-moldlayer is made of a nano metal ion / PVC composite material and comprises the following components in parts by weight: 100 parts of PVC, 0.5-2 parts of nano metal ions, 3.5-5.5 parts of a stabilizer, 8-12 parts of chlorinated polyethylene, 0.5-1.5 parts of stearic acid, 1-3 parts of lead stearate, 0.2-0.8 part of polyethylene wax, 0.5-2 parts of processing aids, 0.5-1 part of a composite coupling agent, 1-5 parts of a macromolecule load and 0.5-4 parts of a macromolecule compatilizer. The production method comprises the following steps: mixing raw materials of the antibacterial anti-mold layer,mixing materials of the PVC composite material layer, and preparing the double-layer antibacterial anti-mold water drainage PVC tube. The antibacterial anti-mold water drainage PVC tube has a very good growth inhibition function on aspergillus niger, aureobasidium pullulans, penicillium funiculosum chaetomium globosum, gliocladium viride and the like, is simple in process, low in manufacturing cost, long-lasting and stable in effect and applicable to industrial popularization and production.

The invention relates to the technical field of antibiological inoculants for treating potato black scurf, in particular to application of penicillium funiculosum in preparing a drug for treating or preventing the potato black scurf. The application has the advantages that the effect of penicillium funiculosum preventing and treating the potato black scurf is obvious, and the defect of preventing and treating of the potato black scurf in the traditional method is overcome; the adopted bacteria strains are widely distributed, the obtaining is easy, and the penicillium funiculosum is suitable for large-scale popularization.

The invention discloses a biocontrol fungus-Penicillium funiculosum S01324 isolated and screened from cysts of oat cyst nematodes. The penicillium fungus S01324 strain of the present invention has a good biocontrol effect on oat cyst nematodes, can parasitize oat cyst nematode cysts, and the parasitic rate reaches 100%; it has strong inhibition on cyst hatching and second-instar larvae Effect. The penicillium fungus S01324 strain provided by the invention has good control effect and stable control effect after dilution, is friendly to the environment, easy to cultivate the strain, has simple fermentation process for large-scale production, and low production cost.

The present invention relates to a method for obtaining a high titer of enzyme mixture comprising cellulases, hemicellulases and β-glucosidases in reutilization of waste water generated during hot water extraction of lignocellulosic biomass or biorefinery waste water using Penicillium funiculosum MRJ-16 mutant strain. The cellulose or lignocellulosic biomass used in the fermentation process is selected from the group consisting of rice straw, wheat straw, corn stover, cotton stalk or a combination thereof. The enzyme mixture obtained by the present process is used for the saccharification of acid pretreated lignocellulosic biomass.

The invention discloses a method for preparing a bioflocculant and use thereof. The method comprises the following steps of: (a) performing fermentation culture on penicillium funiculosum to obtain fermentation liquor and centrifuging the fermentation liquor to remove mycelium to obtain supernate; and (b), mixing the supernate with ethanol and centrifuging the mixture to obtain the bioflocculant. The bioflocculant can be used for treating activated sludge, fly ash, charcoal, ink, muddy water, river sediment suspension, kaoline suspension, manure water, dyeing and printing wastewater and / or fruit juice.

The invention aims at providing an acidic xylanase mutant and an application thereof. Through screening of a plurality of mutants on xylanase of penicillium funiculosum, three mutation sites N104D, T128G and Y129Q can cause improvement of the heat resistance of the xylanase. The optimal reaction temperature of wild xylanase XynH2 provided by the invention is 50 DEG C; the optimal reaction temperatures of mutants XynH2-1 and XynH2-2 are 55 DEG C; the optimal reaction temperature of a mutant XynH2-3 can be 60 DEG C; under the condition of over 53 DEG C, the relative enzyme activity levels of the three mutants are significantly higher than those of the wild mutant; treatment is carried out at 65 DEG C for 5 minutes; the remaining enzymatic activity of the wild xylanase XynH2 only is 5%; and the remaining enzymatic activities of the xylanase mutants XynH2-1, XynH2-2 and XynH2-3 are respectively 20%, 35% and 46%, so that the heat resistance of the screened xylanase mutant is significantly improved.

The invention relates to the technical field of peanut planting, in particular to a continuous-cropping-resistant bacterial liquid as well as a preparation method and application thereof. The continuous-cropping-resistant bacterial liquid comprises 3-5 parts of bacillus megatherium fermentation liquor, 5-7 parts of Penicillium funiculosum fermentation liquor, 5-7 parts of trichoderma viride fermentation liquor, 3-5 parts of pseudomonas fragi fermentation liquor, 40-50 parts of glucose solution with the concentration of 5-7% and 10-20 parts of l-lysine hydrochloride. When the continuous-cropping-resistant bacterial liquid is used for preventing obstacles caused by continuous cropping of peanuts, diseases caused by continuous cropping can be remarkably reduced, the yield of the peanuts can be increased, and the content of total sugar in the peanuts can also be improved.

A method for preparing a hyper-cellulolytic catabolite derepressed mutants of ascomycetes fungus, especially variants of Penicillium funiculosum. Selection media used to isolate such variants include amorphous cellulose and a high concentration of glucose. Cellulase activities of mutant ID-10, in particular such as FPase and β-glucosidase were 1.5 times higher than Penicillium funiculosum MRJ-16 (parent). Furthermore, fungal mutant morphology was changed and no pH adjustment was required throughout the enzyme production process.

The invention provides a method for detecting characteristic fungus Amorphotheca resinae in a jet fuel. According to the method, a set of LAMP primers is designed, a detection system with a reaction temperature of 63 DEG C and a time of 35min and directly observed by an LFD test strip is established, and then specificity and sensitivity evaluations are carried out. Results show that the lowest detection lines of detecting A.resinae genome DNA and a pure culture by applying an LAMP-LFD method are 26fg / muL and 8chu / mL respectively; in specificity confirmatory experiments, four fungi namely trichoderma viride, penicillium funiculosum, aspergillus niger and aureobasidium pullulans found in the jet fuel, and A.resinae are used as specificity test strains, and the LAMP-LFD method detection only shows a positive reaction for A.resinae. The detection method disclosed by the invention is capable of rapidly detecting A.resinae in the jet fuel, and visualized in result. The method is short in time consumption, simple and convenient to operate, high in specificity, high in sensitivity and independent of special detection equipment, as well as expected to be used for on-site field detection for characteristic fungus A.resinae in the jet fuel as a new detection method.

The invention relates to the technical field of antibacterial anti-mildew materials, and discloses a polymer antibacterial anti-mildew material. According to the polymer antibacterial anti-mildew material and application thereof to base paper of tipping paper, the antibacterial anti-mildew material is finally prepared by adding 2-5 parts of Chinese eaglewood, 20-30 parts of radix glycyrrhizae, folium artemisiae argyi and the like. The antibacterial anti-mildew material has the antibacterial anti-mildew functions of the Chinese eaglewood, radix glycyrrhizae and folium artemisiae argyi, and whenthe antibacterial anti-mildew material is applied to tipping paper, the tipping paper obtains excellent anti-mildew functions. In vitro test research founds that 45% alcohol extract of folium artemisiae argyi with a concentration of 125 mg / mL has antibacterial activity on aspergillus versicolor, Botrytis, aspergillus ustus, aspergillus terreus, Penicillium oxalicum, Penicillium rugulosum, penicillium purpurogenum, Penicillium funiculosum, penicillium cyclopium, fusarium and the like. The polymer antibacterial anti-mildew material inherits excellent antibacterial performance of folium artemisiae argyi and other raw materials, enables the base paper of the tipping paper to have good antibacterial anti-mildew effects when applied to base paper of tipping paper, and is harmless to human body.

The invention provides handmade tea tree coconut oil liquid laundry soap and a preparation method thereof. The liquid laundry soap is prepared from the following raw materials in parts by weight: 90 to 100 parts of mixed oil, 20 to 25 parts of potassium hydroxide, 200 to 280 parts of water, 2 to 5 parts of tea tree essential oil and 3 to 5 parts of an auxiliary agent, wherein the mixed oil is prepared from the following raw materials in parts by weight: 80 to 90 parts of coconut oil, 5 to 10 parts of camellia oil and 5 to 12 parts of canola oil; the auxiliary agent is prepared from, by weight,5 to 12 parts of a honeysuckle flower extract and 3 to 8 parts of a propolis extract. A large proportion of the coconut oil is added so that the decontamination capability is improved, the tea tree essential oil is added so that the yellow spot removal capability and the sterilization capability are improved, the liquid laundry soap has obvious inhibition effects on Staphylococcus aureus, Escherichia coli, Aspergillus niger, Trichoderma viride, Penicillium funiculosum, Chaetomium globosum and the like, and the obvious sterilization effect is achieved.

The present invention relates to a mutant fungal strain of Penicillium funiculosum ‘MRJ-16’ characterized by the ability to produce high titer of enzyme mixture comprising FPase, CMCase, Cellobiase, β-glucosidase, endoglucanase, α-L arabinofuranosidase, β-xylosidase, xylanase, pectinase, cellobiohydrase and oxidases and produce enzymes in the presence of a catabolite repressor molecule like glucose and / or xylose. The titer of enzyme mixture produced using mutant fungal strain MRJ-16 is at least two fold higher than naive Penicillium funiculosum strain NCIM 1228, when used in a fermentation process. The mutant strain ‘MRJ-16’ with high hydrolytic activity and catabolite derepressed character is having application in the method of degrading or saccharifying biomass to produce valuable products for example-bioethanol.

The present invention relates to a process for simultaneous production of citric acid and cellulolytic enzymes. The batch process comprising (i) adding slurry of a pre-treated lignocellulosic biomass or cellulose in a fermentation media; (ii) inoculating 10% (v / v) active liquid seed culture of Penicillium funiculosum MRJ-16 in the fermentation media of step (i); (iii) subjecting the culture of step (ii) to fermentation in an aerated fermenter at a temperature of 28-33° C. for a duration of 96 hours; and (iv) collecting enzyme broth after fermentation of step (iii) to obtain citric acid and cellulolytic enzymes. The batch and fed-batch process of the present invention results in high titer production of citric acid and cellulolytic enzymes in a single step and using single microbial strain.

The present invention relates to a method of cellulases enzymes production by Penicillium funiculosum MRJ-16 using minimum media components and / or low cost media components like cellulose or pretreated lignocellulosic biomass as carbon source and soya flour or defatted soya flour or de-oiled soya cake as nitrogen source. The present invention also provides a method for production of high titer of cellulases and hemicellulases enzymes using Penicillium funiculosum MRJ-16 using minimum media and / or low cost media components.