Dry fungus metabolite powder and fertilizer for promoting plant growth, and preparation methods and application thereof
A technology for promoting plant growth and metabolites, applied in the field of fertilizer preparation, can solve problems such as failure to meet plant growth requirements and single fertilizer efficiency, and achieve the effects of increasing the number of effective ears and 1000-grain weight, increasing crop yield, and increasing leaf length.
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[0029] The present invention also provides a method for preparing plant growth-promoting fungal metabolite dry powder, comprising:
[0030] The dry powder of the metabolite of Penicillium fungus, the dry powder of the metabolite of Penicillium restrictum and the dry powder of the metabolite of Penicillium rugosa are mixed to obtain the dry powder of the metabolite of the fungus.
[0031] In the present invention, preferably, the activated Penicillium funiculum, Penicillium limitiosa and Penicillium rugosa are respectively inoculated into the liquid culture medium, shaken and cultivated to obtain the Penicillium fungus metabolic bacteria liquid, the Penicillium limitum metabolic bacterial liquid and Penicillium rugosa Metabolizing bacteria liquid. In the present invention, there is no special limitation on the activation methods of Penicillium funiculum, Penicillium limitiosa and Penicillium rugosa, and conventional activation methods in the field can be used. In the present i...
Embodiment 1
[0045] Embodiment 1 (in the present embodiment, the activation of Penicillium fungus, Penicillium limitatum and Penicillium rugosa, each Penicillium of processes such as inoculation is carried out separately)
[0046] Prepare medium:
[0047] Cha's medium: 30.0g sucrose, NaNO 3 3.0g, MgSO 4 ·7H 2 O 0.5g, KCl 0.5g, FeSO 4 4H 2 O0.01g, K 2 HPO 4 1.0g, 1.0L distilled water, pH 7.0~7.2;
[0048] Malt extract medium: malt extract 20.0g, tap water 1.0L, natural pH.
[0049] The above-mentioned culture medium was sterilized at 121° C. for 20 minutes in an autoclave.
[0050] After the culture medium is cooled, scrape the colonies of Penicillium funiculum, Penicillium limitiosa and Penicillium rugosa respectively into a 250mL Erlenmeyer flask filled with 100mL sterile water, under the condition of 120r / min, Shake for 30 minutes to fully disperse the spore mass. The culture temperature of Penicillium funiculosa is 28°C, and the culture temperature of Penicillium limitatum and ...
Embodiment 2
[0055] By fungal metabolite dry powder and tobacco special fertilizer (N:P in embodiment 1 2 o 5 :K 2 O=10:15:25) composition.
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