36 results about "Enzyme family" patented technology

A process is provided for producing target concentrations of peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity under conditions where control of reaction pH by selection of buffer concentration and concentration of perhydrolase and reactants produces a targeted concentration of peroxycarboxylic acids. The present perhydrolase catalysts are classified as members of the carbohydrate esterase family 7 (CE-7) based on the conserved structural features. Further, disinfectant formulations comprising the peracids produced by the processes described herein are provided, as are corresponding methods of use.

A process is provided for producing target concentrations of peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity under conditions where control of reaction pH by selection of buffer concentration and concentration of perhydrolase and reactants produces a targeted concentration of peroxycarboxylic acids. The present perhydrolase catalysts are classified as members of the carbohydrate esterase family 7 (CE-7) based on the conserved structural features. Further, disinfectant formulations comprising the peracids produced by the processes described herein are provided, as are corresponding methods of use.

The invention discloses an OcPP2C44 gene separated and cloned from rice deoxyribonucleic acid (DNA) fragments and related to rice stress resistance. The protein of the genetic code contains protein phosphatase family conservative catalytic structural domain and is a member of a PP2C type of the gene family. The OsPP2C44 gene is performed with inducible expression of high salinity, low temperature, high temperature and abscisic acid (ABA), and overexpression OsPP2C44 can improve osmotic stress resistance capability of rice during seedling stage and is related to rice stress resistance. The rice gene of the OcPP2C44 gene has obvious response to adverse situation, can be applied to plant stress resistance breeding, and improves stress resistance of plants.

The present invention relates to compositions that can be used in hydrolyzing biomass such as compositions comprising a polypeptide having glycosyl hydrolase family 61 / endoglucanase activity, methods for hydrolyzing biomass material, and methods for reducing viscosity of biomass mixture using a composition comprising a polypeptide having glycosyl hydrolase family 61 / endoglucanase activity.

The invention provides application of delinearized ubiquitin enzyme family with sequence similarity 105, member B (FAM105B) in liver cancer diagnosis, treatment and prognosis judgment. Various technical detection finds that the FAM105B is highly expressed in a liver cancer cell line and liver cancer tissue, and high expression of the FAM105B is positively correlated with adverse clinical pathological characteristics of a hepatocellular carcinoma (HCC) patient, a poor postoperative overall survival rate and a poor tumor-free survival rate; it is proved that high expression of the FAM105B in theliver cancer tissue is an independent risk factor influencing long-term survival of liver cancer patients; an in-vitro hepatoma cell model constructed by gene knockout and overexpression techniques finds that the FAM105B can promote the proliferation, migration and invasion abilities and epithelial-mesenchymal transition of hepatoma cells. The research findings indicate that the delinearized ubiquitin enzyme FAM105B can be used as a marker for developing a new liver cancer diagnosis and prognosis judgment kit, and an effective target is provided for preparing liver cancer treatment medicines.

Compositions and methods for raising the level of HDL cholesterol and apolipoprotein AI in a patient and for lowering the levels of VLDL cholesterol and LDL cholesterol in a patient, including compositions and methods which effect the expression of a gene, LIPG, which encodes a lipase enzyme that is a member of the triacylglycerol lipase family or which effect the enzymatic activity of the enzyme.

The present invention relates to a method for producing highly-concentrated L-amino acid and riboflavin simultaneously, and a microorganism for simultaneously producing L-amino acid and riboflavin. Specifically, the present invention relates to a modified microorganism for producing L-lysine or L-threonine, and riboflavin simultaneously, wherein the microorganism belonging to Corynebacterium sp. capable of producing L-lysine or L-threonine is modified by enhancing the activity of an enzyme family expressed by a rib operon which contains riboflavin biosynthesis gene family. Also, the present invention relates to a method for the simultaneous production of L-lysine or L-threonine, and riboflavin using the modified microorganism, and relates to a formulation or granular formulation, feed, and feed additive, containing L-lysine or L-threonine, and riboflavin produced from a culture medium of the modified microorganism.

The invention provide a group of cysteine hydrolases with (+) gamma lactam resolution activity. The cysteine hydrolases come from 13 microorganism species, and have three common conserved structural domains: the conserved structural domain I: *A(V / M / R / G / T)L(V / H / I)L(V / I)L(V / I)V(N / E / D)*(H / I)DM(L / V / I)Q, the conserved structural domain II: *S(G / D / N)*F(W / Y)*, the conserved structural domain III: *(F / V / M)V(A / C / M / T)G*T(A)N(E / H / D / A)*C(G / A / D / P), wherein * stands for any amino acid residue, and the amino acids in the brackets stand for possible amino acid residues at the same amino acid residues. The invention also provides coding genes of the above cysteine hydrolases, and applications of the above cysteine hydrolases in resolution of racemate gamma lactam.

The invention discloses the application of a soybean E3 ubiquitin ligase family gene GmRNF1a. Soybean GmRNF1a protein coding gene GmRNF1a, its nucleotide sequence is: SEQ ID NO.1. The constructed plant overexpression vector pMDC83‑GmRNF1a was heterologously expressed in the wild type of Arabidopsis, and it was found that the maturation process of the transgenic plants was significantly accelerated, and the fruit pods burst earlier. It shows that the gene can be introduced into plants as a target gene, and by inhibiting the expression of GmRNF1a gene, it can inhibit the early bursting of fruit pods of transgenic plants. It can be seen that the soybean GmRNF1a protein coding gene GmRNF1a of the present invention can be used in promoting early maturity of plants through genetic engineering and regulating the bursting time of plant pods.

The present invention relates to the control of infestation of pests by inhibiting or reducing the expression of genes of the family of chitin synthase. The invention further provides methods and compositions for controlling pests by feeding them with one or more double-strand RNA molecules provides by the present invention. The invention further describes a method of obtaining transgenic plants that express double-strand RNA molecules. The present invention is preferably used for cotton-plants.

The invention provides a beta-glucosaccharase gene for coding a glycosyl hydrolase family 1 and an application thereof. The nucleotide sequence of the beta-glucosaccharase gene is shown in SEQ ID NO:1, the nucleotide sequence of beta-glucosaccharase S-bgl4 coded by the gene is shown in SEQ ID NO:2, and the beta-glucosaccharase S-bgl4 can be applied to decomposition of cellobiose.

The invention discloses streptomyces thermoviolaceus chitinase (GH18 family) as well as a preparation method and application thereof. According to the streptomyces thermoviolaceus chitinase disclosedby the invention, a full-gene synthesis method is utilized to obtain an encoding gene sequence of chitinase in streptomyces thermoviolaceus, and an optimized nucleotide sequence is shown by SEQ ID NO.2; furthermore, a pichia pastoris expression system is utilized to perform high-efficiency secretory expression on an optimized chitinase encoding gene to obtain the streptomyces thermoviolaceus chitinase (GH18 family), and an amino acid sequence of the streptomyces thermoviolaceus chitinase is shown by SEQ ID NO. 1. The obtained streptomyces thermoviolaceus chitinase (GH18 family) disclosed by the invention has higher hydrolytic activity on a low deacetyiation degree chitosan substrate; crude enzyme liquid generated by fermentation in a shaking flask has hydrolytic capacity of degrading 0.5gof chitosan by 1mL crude enzyme liquid (a protein content is about 0.20mg); however, about 100mg of commercial cellulase is needed to degrade the same amount of chitosan; theoretically, an efficiencyis improved by about 500 times; the streptomyces thermoviolaceus chitinase (GH18 family) has good industrial application prospect.