40 results about "Orf virus" patented technology

The invention discloses a passage culture type sheep testis cell and a passage domesticating culture method, and a special culture system and application thereof, and provides a passage culture type sheep testis cell LT-1 (CGMCC No.12988) and a method for obtaining the cell by domesticating culture. The method has the advantages that optimized cell culture liquid is used for performing passage domesticating culture on a sheep testis primary cell (LT), the passage times of the sheep testis primary cell can be increased, and the method is suitable for batched production; the sheep orf virus is subject to multiplication culture by the cell; proofed by results, the sheep orf virus can be continuously subject to passage culture for three generations, the obvious lesion is produced, the content of virus reaches 107.5TCID50 / mL, and the method can be applied to the multiplication culture and vaccine preparation of the sheep orf virus.

The invention discloses a sheep pox and aphtha bigeminy cell attenuated vaccine and a preparation method and application thereof. The effective ingredient of the sheep pox and aphtha bigeminy cell attenuated vaccine is sheep pox virus and contagious pustular dermatitis virus cell passage attenuate virus or passage virus thereof which are separated through the inventor and obtained after passage attenuate culture, wherein the sheep pox virus cell passage attenuate virus is Sheeppox Virus GS-WW 2010 F44 strain, the preservation number of CCTCC NO: V201504, the sheep pox virus (contagious pustular dermatitis virus) cell passage attenuate virus is Orf Virus HB-TS09F65 strain, and the preservation number is CCTCC NO:V201406. Toxin attacking protection test results show that the sheep pox and aphtha bigeminy cell attenuated vaccine is safe and capable of producing effective immune protection force. Accordingly, the sheep pox and aphtha bigeminy cell attenuated vaccine is of an important practical significance in researching safe and efficient attenuated vaccine and preventing and controlling sheep pox and aphtha bigeminy.

The invention provides a goatpox and sheep pox bivalent cell attenuated vaccine. The goatpox and sheep pox bivalent cell attenuated vaccine comprises effective ingredients, namely, a goatpox virus cell passage attenuation virus and a sheep pox virus cell passage attenuation virus, wherein the goatpox virus cell passage attenuation virus is a goatpox virus cell passage attenuation vaccine strain Goatpox Virus HN-XY2010F43 strain CCTCC V201503, and the sheep pox virus cell passage attenuation virus is a sheeppox virus cell passage attenuation vaccine strain Sheeppox Virus GS-WW2010F44 strain CCTCC V201504. The goatpox and orf virus bivalent cell attenuated vaccine is safe, can have the effective immune protection force and has the important practical significance in development of safe and efficient attenuated vaccines as well as prevention and control on goatpox and sheeppox through large-scale production of goatpox and sheep pox virus bivalent cell attenuated vaccines by the aid of the attenuation virus strains.

The invention provides a detection method for detecting the orf virus of sheep. Real-time fluorescent quantitative PCR (polymerase chain reaction) detection is performed on a DNA (deoxyribonucleic acid) template of a sample by designing a pair of primers and a probe. The invention further provides a kit for detecting the orf virus of the sheep by utilizing the detection method. The real-time quantitative method which is established against H2L genes in a conserved region of the ORFV (orf virus) is high in specificity and good in stability. The kit for detecting the orf virus of the sheep, provided by the invention, is simple and convenient to operate, a user only needs to add DNA of the sample to be detected into a reaction tube, detection results can be obtained according to an amplification curve, the operation is time-saving and labor-saving, the cost is lower, and the time is shortened from original 3-4 hours to 1-2 hours; and furthermore, the sensitivity is higher than that of the ordinary PCR, and the detection method and the kit can be used for detecting the low content of the orf virus of the sheep, can be suitable for all levels of prevention and control units, basic-level veterinary stations, large and medium-sized breeding farms and the like in China, and has better application prospects.

The invention discloses a goatpox, sheeppox and contagious ecthyma triple cell attenuated vaccine and a preparation method and application thereof. The effective components of the vaccine are separated by an inventor automatically, and after attenuation culture is conducted, goatpox virus, sheeppox virus and Orf virus cell passage attenuated viruses or passage viruses thereof are obtained, wherein a goatpox virus is a Goatpox Virus HN-XY2010F43 strain, a sheeppox virus is a Sheeppox Virus GS-WW 2010 F44 strain, and an Orf virus is an Orf Virus HB-TS09F65 strain. It is shown through attacking protection test results that the prepared goatpox, sheeppox and contagious ecthyma triple cell attenuated vaccine is safe and capable of producing an effective immune protection force, and important practical significance in developing safe and efficient goatpox, sheeppox and contagious ecthyma triple cell attenuated vaccine and preventing and controlling goatpoxes and ecthyma is achieved.