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Orf virus attenuated strain and application thereof

A kind of technology of oral ulcer virus and attenuated strain, applied in the direction of virus, antiviral agent, virus antigen components, etc., can solve the problems of spreading virus virulence, no vaccine for oral ulcer, unsatisfactory effect of attenuated vaccine, etc.

Inactive Publication Date: 2020-05-12
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is no specific medicine for the treatment of aphthous ulcers, so the prevention of aphthous sores is particularly important. Vaccines for aphthous aphthous ulcers have been researched into virulent scab vaccines, inactivated vaccines, and attenuated vaccines, but so far, inactivated vaccines The attenuated vaccine also has the risk of spreading the virus and reversing the virulence. The attenuated vaccine used in the immunization process is still unsatisfactory and the protection rate is not high. So far there is no effective vaccine for the prevention and treatment of aphthous ulcers

Method used

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  • Orf virus attenuated strain and application thereof
  • Orf virus attenuated strain and application thereof
  • Orf virus attenuated strain and application thereof

Examples

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Effect test

Embodiment 1

[0018] This example provides a method for isolating and identifying an attenuated strain of oropharynx virus. The attenuated strain is QFnm2015 strain, which has been submitted for preservation in China Center for Type Culture Collection, and the preservation number is CCTCC NO: V201903. The attenuated strain is obtained by passing a virulent strain of oropharynx virus for 90 generations. The virulent strain is the attenuated QD / 2015 strain of oropharynx virus. : V201963. The specific method includes the following steps:

[0019] 1. Isolation and identification of virulent strains

[0020] 1 material preparation

[0021] 1.1 Disease data

[0022] Sheep aphthous scab: Collected from an Orf-infected farm in Qingdao, mainly collecting the scabs from the lips and mouth of sick sheep with obvious lesions. (Preserved by the Laboratory of Infectious Diseases, Inner Mongolia Agricultural University)

[0023] 1.2 Virus strains

[0024] ORFV / QD / 2015: The scabs obtained from step 1...

Embodiment 2

[0130] Aphthous Oral Virus Related Test

[0131] 1. Fluorescence quantification of aphthous ulcer virus

[0132] 1.1 PCRTaqMan probe: provided by the Department of Infectious Diseases, College of Veterinary Medicine, Inner Mongolia Agricultural University.

[0133] 1.2 Main reagents

[0134] MEM, trypsin fetal bovine serum, etc. were purchased from Gibco, hydrolyzed milk protein, normal saline, formaldehyde, etc. were prepared by the laboratory, viral genome DNA extraction kit was purchased from Tiangen Biochemical Technology Co., Ltd., Montanide ISA 201 VG adjuvant, Montanide ISA 11 R VG adjuvant was purchased from Seppic Company in France, erythrocyte lysate was purchased from Solarbio Technology Co., Ltd., and CD4+ and CD8+ fluorescent monoclonal antibodies were purchased from BD Company in the United States.

[0135] 1.3 Main instruments

[0136] HW.SY11-K2 electric heating constant temperature water bath, BJ-2CD double-person single-sided vertical purification workbenc...

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Abstract

The invention provides an orf virus attenuated strain and an application thereof. The attenuated strain is an orf virus attenuated strain QFnm2015, the preservation institution is the China Center forType Culture Collection, and the preservation number is CCTCC NO: V201903. According to the invention, the isolated orf virus is proliferated and cultured on lamb testicular cells, and the orf virusattenuated strain is cultured through continuous passage. The isolated strain of the orf virus provides a high-quality material basis and selection space for developing an effective vaccine of endemicORFV, and has important significance for prevention and treatment of orf.

Description

technical field [0001] The invention belongs to the technical field of marbofloxacin pharmaceutical preparations, and in particular relates to an attenuated strain of aphthous oral ulcer virus and its application. Background technique [0002] Orf (Orf) is also known as sheep contagious impetigo (CE), contact contagious pustular stomatitis, and it is mainly a kind of contact zoonotic disease caused by sheep mouth ulcer virus (Orfv). ORF is highly contagious and has a high incidence. The disease is widely distributed, and the countries that raise sheep basically have relevant reports about the occurrence of the disease. The occurrence of the disease has caused serious economic losses to the breeding, and the sheep breeding industry in my country has been affected. Humans can also be infected with Orfv. The main symptoms are pimples on the hands, nose, lips and face. People with immune disorders may have complications such as atypical hyperplasia and autoimmune disorders. Or...

Claims

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Application Information

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IPC IPC(8): C12N7/04C12N7/08A61K39/275A61P31/20C12R1/93
CPCC12N7/00A61K39/12A61P31/20C12N2710/24221C12N2710/24234C12N2710/24264A61K2039/5254A61K2039/552
Inventor 张静涂明亮李娜仲亮安维雪李智马园张七斤
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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