The invention relates to the technical field of extracting ARA oil from a
Mortierella alpine
fermentation liquor, and concretely relates to a method for extracting arochidonic acid oil in a non-
solvent manner. The method which can improve the wall breaking effect on
Mortierella alpine, and is simple in process, feasible in industry, capable of easily achieving large-scale production, high in oil yield, free of
solvent residues, green and efficient. The method comprises the following steps: S1, separating cells; S2, carrying out air
cavitation wall breaking; S3, mixing materials; S4, puffing and granulating; S5, adding water, and dispersing; S6, carrying out secondary wall breaking treatment; S7, separating ARA
crude oil; and S8, separating and
drying. A plurality of wall breaking
modes arecombined to carry out sufficient wall breaking on
Mortierella alpine cells, and no
organic solvent is introduced, so the problem of
solvent residues in an existing product is solved, some non-polar
cell metabolites can be prevented from being brought into crude arochidonic acid oil through non-
solvent extraction, the quality of the crude arochidonic acid oil is improved, and the refining pressureis reduced.