Microbial oil rich in Sn-2-site ARA as well as preparation method and application of microbial oil

A microbial oil, sn-2 technology, applied in the field of microorganisms, can solve the problem of low fatty acid absorption rate, and achieve the effect of improving the absorption rate and promoting absorption and utilization.

Active Publication Date: 2020-02-04
瞿瀚鹏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the problem that the human body has a low absorption rate of fatty acids due to the high ratio of Sn-1 and Sn-3 fatty acids in triglycerides in microbial oils in the prior art, and to provide a kind of fatty acid rich in Sn-2 The microbial oil of ARA and its preparation method and application, the microbial oil is produced by fermentation of Mortierella alpina, and the proportion of ARA at the Sn-2 position of the triglyceride in the oil is not less than 23%, which effectively improves the human body absorption rate of ARA

Method used

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  • Microbial oil rich in Sn-2-site ARA as well as preparation method and application of microbial oil
  • Microbial oil rich in Sn-2-site ARA as well as preparation method and application of microbial oil
  • Microbial oil rich in Sn-2-site ARA as well as preparation method and application of microbial oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) respectively inoculate the Mortierella alpina conventional strain and the Mortierella alpina strain used in the present invention (preservation number is GDMCC No.60734) on the slope of a PDA test tube and cultivate them at 27°C for 5 days, and transfer the generated spores Cultivate on the slant of PDA medium eggplant bottle at 27°C for 4 days until mature spores are generated, remove the hyphae and spores on the medium, and prepare a spore suspension with 20 mL of sterile water.

[0049] (2) the spore suspension of step (1) gained is inoculated into the shaking flask that is placed with seed culture medium and cultivates and obtains seed liquid, and the inoculum amount is connected with the mode of 2-3 triangular flasks by every PDA eggplant bottle seed, puts Cultivate on a shaker at a temperature of 27°C and a shaking speed of 180r / min for 4 days. The carbon source in the seed medium is glucose 50g / L, the nitrogen source is yeast powder 15g / L, and the pH is 7.2. ...

Embodiment 2

[0058] (1) respectively inoculate the Mortierella alpina conventional strain and the Mortierella alpina strain used in the present invention (preservation number is GDMCC No.60734) on the slope of a PDA test tube and cultivate it at 28°C for 6 days, and transfer the generated spores Cultivate at 28°C for 5 days on the slant of the PDA medium eggplant bottle until mature spores are generated, remove the mycelia and spores on the medium, and prepare a spore suspension with 20 mL of sterile water.

[0059] (2) the spore suspension of step (1) gained is inoculated into the shaking flask that is placed with seed culture medium and cultivates and obtains seed liquid, and the inoculation amount is by the mode that every eggplant bottle seed connects 2-3 triangular flasks, puts Cultivate on a shaker at a temperature of 28°C and a shaking speed of 200r / min for 5 days. The carbon source in the seed medium is glucose 60g / L, the nitrogen source is yeast powder 10g / L, peptone 10g / L, and the...

Embodiment 3

[0067] (1) respectively inoculate the Mortierella alpina conventional strain and the Mortierella alpina strain used in the present invention (preservation number is GDMCC No.60734) on the slope of a PDA test tube and cultivate it at 29°C for 7 days, and transfer the generated spores Cultivate at 29°C for 6 days on the slant of the PDA medium eggplant bottle until mature spores are generated, remove the hyphae and spores on the medium, and prepare a spore suspension with 20 mL of sterile water.

[0068] (2) the spore suspension of step (1) gained is inoculated into the shake flask that shake flask seed culture medium is placed and cultivates and obtains seed liquid, and inoculum amount is connected to 2-3 triangular flasks by every PDA eggplant bottle seed method, placed on a shaker at a temperature of 29°C and a shaking speed of 190r / min for 6 days, the carbon source in the seed medium was glucose 60g / L, starch 10g / L, the nitrogen source was yeast powder 25g / L, and the pH was ...

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Abstract

The invention relates to the technical field of microorganisms, and discloses microbial oil rich in Sn-2-site ARA as well as a preparation method and application of the microbial oil. The proportion of ARA at the Sn-2 site of triglyceride in the microbial oil is not lower than 23%. The preparation method of the microbial oil comprises the step of inoculating a fermentation medium with a mortierella alpina strain for fermentation to obtain the microbial oil, wherein the mortierella alpina strain has preservation number of GDMCC No.60734. The proportion of the ARA at the Sn-2 site of the triglyceride in the microbial oil is remarkably higher than that of the ARA at the Sn-1 site and the Sn-3 site of the triglyceride, so that the human body absorption rate of the ARA in the microbial oil is remarkably higher than that of the ARA produced by a conventional strain, and absorption and utilization of the functional fatty acid ARA by a human body are promoted.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a microbial oil rich in Sn-2 position ARA and its preparation method and application Background technique [0002] Lipids are one of the indispensable nutrients for the human body. They not only provide energy for the body, but are also indispensable raw materials for the formation of cells and tissues, as well as fat-soluble vitamins. However, in order to play these important roles in the body, it must first be digested and absorbed by the intestinal tract. The digestion and absorption of lipids are mainly carried out in the small intestine. The lipases in the pancreas and small intestine, and cholate in bile are involved in digestion and absorption. , and the alkaline environment formed by the secretion of bicarbonate from the pancreas and bile is also an indispensable environmental condition. The efficiency with which lipids are absorbed by the body depends on the vari...

Claims

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Application Information

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IPC IPC(8): C12P7/64A23L33/115C12R1/645C12P7/6472
CPCC12P7/6472C12P7/6463A23L33/115A23V2002/00A23V2200/30A23V2250/18Y02E50/10A23L29/04A23L29/065C12N1/14C12R2001/645A23L33/40C12N1/145A23D9/04
Inventor 梁云曹晟王身健
Owner 瞿瀚鹏
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