Method for producing arachidonic acid by fermentation of mortierella alpine

A technology of Mortierella alpine and arachidonic acid, applied in the field of microbial fermentation

Active Publication Date: 2020-02-07
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In the prior art, rapeseed meal has not been applied to the fermentation technology of Mortierella alpina. Due to the uncertainty of microbial fermentation, it is unknown whether Mortierella alpina can normally grow and metabolize in rapeseed meal medium

Method used

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  • Method for producing arachidonic acid by fermentation of mortierella alpine

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Experimental program
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Embodiment 1

[0039] This embodiment provides a fermentation method for producing arachidonic acid by Mortierella alpina fermentation, wherein the fermentation medium contains untreated rapeseed meal, and the specific steps are as follows:

[0040] 1. Activation of the original strain: inoculate the Mortierella alpina strain preserved in the ampoule tube on the PDA slant medium, cultivate it at 28±1°C for 8 days, select the PDA medium plate with vigorous mycelium and spore growth, and take All the hyphae and spores on the culture medium were prepared into a spore suspension with sterile water, and the concentration of the spore suspension was not less than 10 6 cfu / mL.

[0041] 2. Seed culture: Inoculate the spore suspension in the shake flask culture medium, shake and cultivate at 28±1°C for 48 hours, and shake the shaking table at a speed of 115 rpm to obtain the seed culture solution. The shake flask culture medium The mass percentages of the main raw material components are glucose 6%,...

Embodiment 2

[0044] Embodiment 2 ferments Mortierella alpina in the same manner as in Example 1, the only difference being that the mass percentages of the main raw material components in the fermentation medium of Example 2 are glucose 8%, yeast extract 0.8%, rapeseed meal Hydrolyzate 1.5%, matrix distilled water, pH value is 7.0, wherein, the preparation method of described rapeseed meal hydrolyzate is as follows: first dilute rapeseed meal with water into a suspension with a solid-to-liquid ratio of 20%, and then at 40°C, In an environment with a pH of 7, neutral protease was added for hydrolysis. The amount of neutral protease added was 4000 U / g, hydrolyzed for 3 hours, and rapeseed meal hydrolyzate was obtained after drying.

Embodiment 3

[0046] Embodiment 3 ferments Mortierella alpina in the same manner as in Example 1, the only difference being that the mass percentages of the main raw material components in the fermentation medium of Example 3 are glucose 8%, yeast extract 0.8%, rapeseed meal Hydrolyzate 1.5%, matrix distilled water, pH value is 7.0, wherein, the preparation method of described rapeseed meal hydrolyzate is as follows: first dilute rapeseed meal with water into a suspension with a solid-to-liquid ratio of 20%, and then dilute the rapeseed meal Hydrolysis was carried out with phytase at 50° C. for 2 hours in an environment with a pH value of 5, the amount of phytase added was 10 U / g, and the rapeseed meal hydrolyzate was obtained by drying after hydrolysis.

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Abstract

The invention provides a method for producing arachidonic acid by fermentation of mortierella alpine, which uses rapeseed meal or rapeseed meal hydrolysate as a main component of a fermentation mediumto ferment and culture the mortierella alpine. According to the fermentation method, the production cost of arachidonic acid is reduced, and the yield of arachidonic acid is increased.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a method for producing arachidonic acid by Mortierella alpine fermentation. Background technique [0002] The output of rapeseed meal in my country is very large, and the theory of rapeseed meal is widely used, but its application scope is still limited to the field of feed. [0003] In the existing applications of rapeseed meal, rapeseed meal is used as a substrate, and solid-state fermentation uses specific bacterial fermentation to hydrolyze rapeseed meal or remove harmful substances in it to achieve detoxification and obtain products that can be used for feed. There are few culture components using rapeseed meal for microbial fermentation. [0004] The application of rapeseed meal in the field of microbial fermentation is relatively limited. First, the high content of cellulose in rapeseed meal affects the utilization of protein by microorganisms; second, beca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/645
CPCC12P7/6427Y02P60/87
Inventor 李翔宇陆姝欢汪志明余超杨艳红
Owner CABIO BIOTECH WUHAN CO LTD
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