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Fermentation medium for promoting mortierella alpina to produce EPA (eicosapentaenoic acid) and application thereof

A fermentation medium, Mortierella alpine technology, applied in the field of medium, can solve problems such as low yield

Active Publication Date: 2017-05-10
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Wild-type Mortierella alpina needs to synthesize EPA at low temperature, and the yield is low

Method used

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  • Fermentation medium for promoting mortierella alpina to produce EPA (eicosapentaenoic acid) and application thereof
  • Fermentation medium for promoting mortierella alpina to produce EPA (eicosapentaenoic acid) and application thereof
  • Fermentation medium for promoting mortierella alpina to produce EPA (eicosapentaenoic acid) and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1: Determination of glucose concentration in fermentation medium

[0044] Mortierella alpina CCFM695 was inoculated into the activation medium with a 1% inoculum amount, activated at 28°C and 200 rpm for 36 hours, and activated for two generations;

[0045] The composition of the activation medium is: 20g / L glucose, 5g / L yeast extract (Yeast extract), 10g / L potassium nitrate, 1g / L potassium dihydrogen phosphate, 0.25g / L magnesium sulfate heptahydrate, using 1M HCl and The pH of its aqueous solution was adjusted to 6.0 with 1M NaOH, and then sterilized at a temperature of 115° C. for 20 minutes to obtain the activated medium.

[0046] Broth culture medium (20g / L glucose, 5g / L yeast extract, 1g / L potassium dihydrogen phosphate, 0.25g / L magnesium sulfate heptahydrate, 10g / L potassium nitrate in prior art record, surplus is water, pH 6.0.) On the basis of investigating whether increasing the glucose concentration is conducive to the growth of Mortierella alpina ...

Embodiment 2

[0053] Embodiment 2: Determination of the type of carbon source

[0054] Replace the glucose in the fermentation medium of Example 1 with cornstarch, soluble starch, potato starch or glycerol of 50g / L respectively, also set the group of each 25g of glucose and soluble starch, other compositions and conditions remain unchanged, the experimental The strain Mortierella alpina CCFM695 was fermented at room temperature at 28°C for 7 days, and the fatty acid content in the samples was determined.

[0055] Table 2 Effects of different carbon sources on the production of EPA by fermentation of Mortierella alpina CCFM695

[0056]

[0057] Note: The data in the table are expressed as mean ± standard deviation, and the significance analysis is carried out by Tukey HSD method in SPSS.

[0058] The results show that Mortierella alpina CCFM695 can use various starches as substrates to ferment lipids to varying degrees, among which corn starch has the best effect, with the highest EPA yi...

Embodiment 3

[0059] Embodiment 3: the determination of organic nitrogen source

[0060] On the premise that the carbon source in the medium is 50g / L glucose, compare the effects of organic nitrogen source yeast extract (Yeastextract, expensive) and soybean meal extract (cheap) on the fatty acid production of Mortierella alpina, in which the yeast extract The concentration in the culture medium is 5g / L, the concentration of soybean meal extract in the culture medium is 50g / L, and other components and conditions remain unchanged. The experimental strain Mortierella alpina CCFM695 is fermented at room temperature at 28°C for 7 days. Determination of fatty acid content in samples.

[0061] Table 3 Effects of different organic nitrogen sources on the production of EPA by fermentation of Mortierella alpina CCFM695

[0062]

[0063] Note: The data in the table are expressed as mean ± standard deviation, and the significance analysis is carried out by Tukey HSD method in SPSS.

[0064] Furthe...

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Abstract

The invention relates to a fermentation medium for promoting mortierella alpina to produce EPA (eicosapentaenoic acid). The fermentation medium for promoting the mortierella alpina to produce the EPA consists of 50g / L of starch, 30-70g / L of soybean meal extract, 0-10g / L of potassium nitrate, 1g / L of phosphate, 0.25g / L of magnesium sulfate heptahydrate and the balance of water, and PH (potential of hydrogen) value of the fermentation medium is 6.0-8.0. The fermentation medium for promoting the mortierella alpina to produce the EPA achieves the purpose of substantially improving EPA yield of the mortierella alpina CCFM695 under normal temperature environment, enables the EPA yield to reach 1.7g / L, and increases the yield by about 3 times of that under original conditions.

Description

[0001] 【Technical field】 [0002] The invention relates to a culture medium, in particular to a fermentation medium containing soybean meal extract and the application of the culture medium in promoting the production of EPA by Mortierella alpina. [0003] 【Background technique】 [0004] EPA (all cis-5, 8, 11, 14, 17-eicosapentaenoic acid) is an omega-3 series long-chain polyunsaturated fatty acid, which has anticoagulant, anticancer, anti-inflammatory, anti-cardiovascular It has special physiological functions such as reducing blood fat and reducing blood fat, and has many applications in the pharmaceutical and health care products, food and feed industries. The biological sources of EPA mainly include marine fish and microbial fermentation production. The production of EPA by microbial fermentation is rich in raw materials, short in cycle, cheap, small in floor area, and not affected by climate, etc., which has attracted more and more attention. At present, the commercial pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12N1/14C12R1/645
CPCC12N1/14C12P7/6427
Inventor 陈海琴陈永泉陈卫葛程凤唐鑫杨芹顾震南张灏
Owner JIANGNAN UNIV
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