Method for extracting grease contained in mortierella alpine

Abstract

The invention relates to a method for extracting grease contained in mortierella alpine. The method comprises the following steps: (1), inoculating mortierella alpine strains into a liquid culture medium for fermentation to obtain fermentation liquor; (2), performing filtering to obtain mortierella alpine wet cells, dissolving the mortierella alpine wet cells in a sodium chloride solution to obtain a mortierella alpine cell solution, then performing emulsifying and shearing, and adjusting the temperature to 40-60 DEG C; (3), adjusting the pH value of the mortierella alpine cell solution to 6.5-7.5, adding an autolysis inducer, continuously performing stirring, and autolyzing mortierella alpine cells for 8-12 hours in a vacuum oxygen-free environment to obtain a mortierella alpine autolysis solution; (4), carrying out centrifugal separation, and collecting upper-layer grease; (5), collecting lower-layer solid obtained by centrifugal separation in the step (4), uniformly mixing the lower-layer solid with purified water, performing centrifugal separation, and collecting upper-layer grease; and (6), mixing the upper-layer grease collected in the steps (4) and (5). The method can effectively extract the grease in mortierella alpine cells, has the advantages of simple technique and low energy consumption, and is beneficial to implementing large-scale production.

Description

technical field [0001] The invention relates to an oil extraction method, in particular to a method for extracting oil contained in Mortierella alpina. Background technique [0002] Polyunsaturated fatty acids (PUFAs) play a vital role in human growth and health. To maintain the normal functions of various tissues, the human body must ensure that there are sufficient fatty acids. If it is lacking, it will cause a series of symptoms, including growth retardation, abnormal skin scales, mental retardation, etc. For example, arachidonic acid (ARA), as an important PUFAs, plays a very significant role in enhancing memory, thinking ability, protecting eyesight and improving intelligence in infants and young children. Long-term lack of ARA in the infant diet can lead to late-onset sepsis in infants and young children. [0003] In the ARA oil manufacturing industry, organic solvents are often used as extractants, followed by vacuum drying to evaporate the organic solvents from the...

AI Technical Summary

Problems solved by technology

If ARA oil is extracted from Mortierella alpina by using alkaline enzyme method to extract oil, the oil will dissociate into the fermentation liquid and easily undergo saponification reaction with alkali, which will reduce the oil extraction rate

[0006] At present, the technology of using microbial fermentation to produce ARA oil is very mature, but there are still some problems mentioned above in the extraction process of microbial fermentation to produce ARA oil, which needs to be improved

The patent application of CN101195813A discloses a process of using high-pressure homogeneously broken cell wall to extract oil. This process needs to use high pressure of 120MPa to break microbial cells many times. The energy consumption required for production is huge, which is not conducive to large-scale production

The patent application of CN 101985637B discloses a method of using microbial autolysis to extract microbial oil. This process requires the use of No. 6 extraction solvent, hexane, heptane or petroleum ether, which brings unstable risks to the production of enterprises , also provides the possibility for organic solvent residual hazards to enter the food chain