Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extracting arochidonic acid oil in non-solvent manner

A kind of arachidonic acid and non-solvent technology, which is applied in the direction of fat production, fat oil/fat refining, fat oil/fat production, etc. It can solve the problems of solvent residue, low extraction cost, and non-solvent extraction due to wall breaking effect, and achieve Effects of reducing refining pressure, solving solvent residue and improving quality

Pending Publication Date: 2020-07-03
RUNKE BIOENG FUJIAN
View PDF7 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Separation and extraction of ARA oil from Mortierella alpina. At present, most methods are used to dry and granulate Mortierella alpina cells, and then use organic solvent to extract. This method has simple process and low extraction cost, but there is a problem of solvent residue. , and organic solvent extraction is easy to extract some other non-polar substances other than ARA oil in Mortierella alpina cells, which increases the difficulty of subsequent ARA oil refining
Moreover, the cell wall of Mortierella alpina is very strong, and the wall-breaking effect of general wall-breaking methods cannot achieve the purpose of non-solvent extraction. Therefore, it is necessary to develop a more effective wall-breaking method, and how to form a simple, industrially feasible, green and efficient method. Method of non-solvent extraction of ARA oils

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Carry out Mortierella alpina 3-stage fermentation culture in 1 ton fermenter, after reaching the fermentation end point, dry matter content is about 0.024kg / kg in the Mortierella alpina fermented liquid, and ARA grease amount (in the Mortierella alpina fermented liquid) dry matter) was 45%, and the ARA content (in total fat) was 47%.

[0029] Filter 600kg of fermented liquid with plate and frame, using 2μm filter cloth, the plate and frame inlet pressure is 0.5MPa, after the pressure filtration is completed, the dry matter content of Mortierella alpina cell wet cells is 30%, and the wet cells of Mortierella alpina About 48kg.

[0030] The wet cells of Mortierella alpina cells were transferred to the air cavitation tower, the air inlet temperature in the tower was 160°C, the outlet air temperature was 83°C, the negative pressure inside the tower was -180Pa, and the fan frequency was 40Hz. The interior of the Mortierella alpine wet cell cavitation tower is pressurized an...

Embodiment 2

[0036] Carry out Mortierella alpina 3-stage fermentation culture in 10 tons of fermentation tanks, after reaching the end of fermentation, the dry matter content in the Mortierella alpina fermentation broth is about 0.0396t / t, and the ARA oil content (in the Mortierella alpina fermentation broth) dry matter) was 48.5%, and the ARA content (in total fat) was 45%.

[0037]Filter 7 tons of fermentation broth with a plate and frame, using a filter cloth of 2 μm, and the plate and frame liquid inlet pressure is 0.45MPa. After the press filter is completed, the dry matter content of the Mortierella alpina cell wet cell is 33%, and the Mortierella alpina wet cell About 830.40kg. The wet cells of Mortierella alpina cells were transferred to the air cavitation tower. The air inlet temperature in the tower was 163°C, the outlet air temperature was 82°C, the negative pressure inside the tower was -190Pa, and the fan frequency was 40Hz. The interior of the Mortierella alpine wet cell cav...

Embodiment 3

[0043] Carry out Mortierella alpina 3-stage fermentation culture in 1 ton of fermenter, after arriving at the end of fermentation, dry matter content is about 0.0396kg / kg in the Mortierella alpina fermented liquid, ARA grease amount (in the Mortierella alpina fermented liquid) dry matter) was 49.75%, and the ARA content was (in total fat) 47.89%.

[0044] Filter 650kg of fermented liquid with plate and frame, adopt 2μm filter cloth, and the plate and frame liquid inlet pressure is 0.5MPa. After the pressure filtration is completed, the dry matter content of Mortierella alpina cell wet cells is 36%, and the wet cells of Mortierella alpina are about It is 71.00kg. The wet cells of Mortierella alpina cells were transferred to the air cavitation tower, the air inlet temperature in the tower was 170°C, the outlet air temperature was 86°C, the negative pressure inside the tower was -184Pa, and the fan frequency was 40Hz. The interior of the Mortierella alpine wet cell cavitation to...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Acid valueaaaaaaaaaa
Povaaaaaaaaaa
Login to View More

Abstract

The invention relates to the technical field of extracting ARA oil from a Mortierella alpine fermentation liquor, and concretely relates to a method for extracting arochidonic acid oil in a non-solvent manner. The method which can improve the wall breaking effect on Mortierella alpine, and is simple in process, feasible in industry, capable of easily achieving large-scale production, high in oil yield, free of solvent residues, green and efficient. The method comprises the following steps: S1, separating cells; S2, carrying out air cavitation wall breaking; S3, mixing materials; S4, puffing and granulating; S5, adding water, and dispersing; S6, carrying out secondary wall breaking treatment; S7, separating ARA crude oil; and S8, separating and drying. A plurality of wall breaking modes arecombined to carry out sufficient wall breaking on Mortierella alpine cells, and no organic solvent is introduced, so the problem of solvent residues in an existing product is solved, some non-polar cell metabolites can be prevented from being brought into crude arochidonic acid oil through non-solvent extraction, the quality of the crude arochidonic acid oil is improved, and the refining pressureis reduced.

Description

technical field [0001] The invention relates to the technical field of extracting ARA oil from Mortierella alpina fermentation broth, in particular to a non-solvent extraction method for arachidonic acid oil. Background technique [0002] Arachidonic acid (ARA) is an n-6 long-chain polyunsaturated fatty acid (PUFA) (5,8,11,14-eicosatetraenoic acid, 20:4). ARA is the most abundant 20-carbon unsaturated fatty acid in the human body. It is an important lipid substance in the human body, and ARA derivatives can regulate lipoprotein metabolism, blood rheology, blood vessel elasticity, blood cell function and platelet activation. ARA must be synthesized by elongation and desaturation of the essential fatty acid linoleic acid (LOA), and the content of △6-desaturase required in the synthesis process is very low, and the yield of synthetic ARA is limited. [0003] The ARA required by the human body must be supplemented by diet, especially during the rapid growth period of the body, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C11B1/02C11B1/06C11B3/16
CPCC11B1/025C11B1/06C11B3/16C11B1/02
Inventor 陈璇李媛媛王文谦郑晓辉陈蝶玲
Owner RUNKE BIOENG FUJIAN
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com