Mortierella elongata PFY capable of utilizing lignocellulose for fermentation production of microbial oil and method of producing microbial oil by same

Abstract

The invention discloses a Mortierella elongata PFY capable of utilizing lignocellulose for fermentation production of microbial oil and a method of producing microbial oil by the same. The method mainly comprises the following steps: culturing and screening strains by using a Congo red culture medium; inoculating the strains into a medium containing lignocellulose for culturing; drying and crushing fermented substances; and extracting crude oil in the dried substances through extraction in an organic solvent. According to ITS complete sequence analysis, the screened strains are Mortierella fungi; the strains grow rapidly and can highly efficiently utilize lignocellulose, the utilization rate of lignocellulose is as high as 90% after fermentation is finished, and the dried substances obtained after fermentation contain a great number of microbial oil, wherein the content of unsaturated fatty acid is 59%.

Description

technical field [0001] The invention relates to a new bacterial strain for fermenting and producing unsaturated fatty acid by using pretreated lignocellulose as a substrate. Background technique [0002] Usually, there are problems such as low production efficiency and high production cost when microbial fermentation is used to process and utilize these waste materials, and it is necessary to combine other physical or chemical methods to pretreat the materials, and sometimes multi-step enzymatic hydrolysis treatment and fermentation are required to obtain products. . Enzymatic hydrolysis and saccharification liquid are used for fermentation production. Although microorganisms can easily use the saccharification liquid produced by enzymatic hydrolysis for fermentation, this requires the addition of cellulase and thus increases production costs. There are many reports on the production of cellulosic ethanol by fermentation of lignocellulose, but there are few reports on the p...

AI Technical Summary

Problems solved by technology

[0002] Usually, there are problems such as low production efficiency and high production cost when microbial fermentation is used to process and utilize these waste materials, and it is necessary to combine other physical or chemical methods to pretreat the materials, and sometimes multi-step enzymatic hydrolysis treatment and fermentation are required to obtain products.

Use enzymatic saccharification liquid for fermentation production, although microorganisms can easily use the saccharification liquid produced by enzymatic hydrolysis for fermentation, but this requires the addition of cellulase and thus increases production costs

There are many reports on cellulosic ethanol as the product produced by fermentation of lignocellulose, but there are few reports on the production of oil by fermentation, and there are fewer studies on the direct fermentation of lignocellulose by a single strain to produce biological products