Mortierella alpina 0901, and application and microbial inoculums thereof

A technology of Mortierella alpina and fungal agent, applied in the direction of application, biocide, fungi, etc., to achieve the effect of ensuring high quality and safety, simple production process and low cost

Active Publication Date: 2020-02-21
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report about the role of Mortierella alpina in agriculture.

Method used

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  • Mortierella alpina 0901, and application and microbial inoculums thereof
  • Mortierella alpina 0901, and application and microbial inoculums thereof
  • Mortierella alpina 0901, and application and microbial inoculums thereof

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Experimental program
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Effect test

preparation example Construction

[0040] The present invention also provides an inoculum. The preparation method of the inoculum comprises: inoculating the Mortierella alpina 0901 described in the above technical scheme on a PDA solid medium for cultivation to obtain Mortierella alpina hypha blocks; Said Mortierella alpina hyphae blocks were inoculated in a PDA liquid medium for shaking culture for 3 to 5 days to obtain a shaking culture; the shaking culture was inoculated into a solid material for cultivation, and when the hyphae of Mortierella alpina were overgrown with the solid material After that, the bacterial agent is obtained.

[0041] In the present invention, the inoculation amount and inoculation method of the Mortierella alpina 0901 on the PDA solid medium are not particularly limited. Conventional methods are sufficient. There are also no special restrictions on the culture conditions. The conventional Mortierella alpina The conditions for culturing on PDA solid medium are sufficient.

[0042] In the ...

Embodiment 1

[0047] Implementation steps:

[0048] Step 1: Inoculate the well-grown Mortierella alpina 0901 (preservation number CGMCC No. 17791) into the PDA liquid medium, and culture it on a shaker at 28°C and 180rpm for 5 days.

[0049] Step 2: Filter the liquid cultured Mortierella alpina under aseptic conditions, and rinse the filtered mycelium with sterile deionized water.

[0050] Step 3: Dissolve 20 g of clean mycelium in 100 mL of sterile deionized water, add the bacterial solution to 10 kg of soil, mix well and put into a pot.

[0051] Step 4: Plant the crops after adequate watering.

Embodiment 2

[0061] Implementation steps:

[0062] Step 1: Inoculate the well-grown Mortierella alpina species into the PDA liquid medium, and cultivate for 5 days in the dark on a shaker at 28°C and 180 rpm.

[0063] Step 2: Inoculate the fermented mixture into the sterilized bran corn flour stalk bean cake powder fulvic acid medium (5 parts of wheat bran, 5 parts of corn flour, 60 parts of corn stalks, 25 parts of soybean meal powder, 5 parts of fulvic acid And 100 parts of water), stand at 28°C and cultivate in the dark until the hyphae completely cover the medium.

[0064] Step 3: Take out the hyphae from different triangular flasks, and mix them well to become the inocula.

[0065] Step 4: Accurately weigh 20g of inoculum, add it to 7.5kg of soil, mix well and put it in a pot.

[0066] Step 5: Plant crops after adequate watering.

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Abstract

The invention provides a mortierella alpina 0901, and an application and microbial inoculums thereof, and belongs to the technical field of microbial inocula. The Latin name of the mortierella alpina0901 is Mortierella alpina, the mortierella alpina is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.17791. The mortierella alpina can obviously improve conversion of soil nitrogen, promote nitrogen absorption of plants and improve biomass of plants. The content of indoleacetic acid in maize root systems at a seedling stage is increased by 70% and the biomass of overground parts of maize is increased by 33%, which indicated that mortierella alpina directly promotes crop growth. The content of abscisic acid in root systems is closely related to drought resistance, salt resistance and the like of crops, and the content of the abscisic acid is increased by 36%. Elongation of root systems is promoted, nutrient absorption of the root systems is promoted, the content of nitrogen, phosphorus and potassium in plants is improved, and the yield of crops is increased by 23%.

Description

Technical field [0001] The invention belongs to the technical field of microbial inoculants, and particularly relates to a Mortierella alpina 0901 strain and its application and inoculants. Background technique [0002] Certain soil fungi promote the growth of crops by stimulating the secretion of crop growth hormones, promoting the conversion of soil nutrients, and increasing the absorption of nutrients by crops. Zygomycetes are a group of saprophytic fungi with high diversity and abundance. In the phylum Zygomycota, Mortierella fungi have the ability to decompose a variety of recalcitrant organic compounds, and can use difficult-to-use organic matter in the soil for growth and reproduction, and at the same time promote the conversion of soil nutrients. It can also resist the growth and reproduction of certain fungi and bacterial pathogens by synthesizing a variety of antibiotics. [0003] Yamada et al. reported for the first time a strain of Mortierella elongata 1S-5 in 1987 an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/30A01P21/00C05F11/08C12R1/645
CPCC05F11/08C12N1/14C12N1/145C12R2001/645
Inventor 韩燕来李世莹李芳王祎李青松谭金芳
Owner HENAN AGRICULTURAL UNIVERSITY
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