31 results about "Heteroantigens" patented technology

The invention relates to a joint detection device and a preparation method of helicobacter pylori urease antibodies IgM and IgG. The helicobacter pylori urease antibodies are prepared from a nitrocellulose membrane, a glass fiber adsorbing a colloidal gold labeled helicobacter pylori urease antigen and a mouse IgG, a sample pad, absorbent paper and other auxiliary materials, wherein the materials are adhered together, and the nitrocellulose membrane contains a purified high-specificity mouse-anti-human IgM and IgG antibodies, and a goat-anti-mouse antibody in a solid phase manner. The joint detection device has the advantages that the structure is simple, the conception is novel, the nitrocellulose membrane are coated with the anti-human IgM and IgG antibodies, so that the specificity is strong, and the helicobacter pylori urease antibodies IgM and IgG in a specimen are simultaneously detected without increasing the production operation complexity. Proper gold spray buffer and sample pad treating fluid are matched to effectively improve the reaction sensitivity on the basis of guaranteeing complete release of immunocolloidal gold, and under the same threshold, the use amount of the immunocolloidal gold can be reduced to save the cost. The detection device is high in sensitivity, strong in specificity, simple, convenient and strong in practicability and can realize the time-saving aim during operation.

The invention provides a prostate specific antigen detection kit, detection method and application, and relates to the technical field of antigen detection. The kit and detection method of the present invention use the secondary antibody to modify the DNA, and connect it with the antigen in an adjacent position to form a Y-shaped antigen-antibody-DNA hybrid chain, which is used as a signal converter to convert the antigen input into the DNA signal output; at the same time, the chain substitution reaction Combined with the hybridization chain reaction, the two-stage amplification of the signal is realized; each adjacent two-terminal sequence (hairpin DNAH3 and hairpin DNA H4) on the long-chain DNA assembles to form a magnesium ion DNase, and the enzyme in the presence of magnesium ions The molecular beacon in the cutting solution, the disconnected molecular beacon is released and emits fluorescence, and the new molecular beacon is combined with magnesium ion DNase to be cut, and the fluorescent signal is cyclically amplified to realize the third round of signal amplification, greatly Improved detection efficiency and sensitivity, enabling a detection limit of 0.73pg mL ‑1 .

The invention relates to a new strain of pigs very suitable for xenotransplantation. The first new porcine line lacks a functional porcine endogenous retrovirus and thus is suitable as a donor for tissue and / or cell xenotransplantation into a human recipient. These pigs can also be used as base pigs for further operation, e.g., by gene editing of heteroantigens to produce a second new strain of pigs that is not only free of infectious porcine retroviruses, but also free of major heteroantigens that cause super-acute organ rejection. These pigs can be used to transplant the entire organ, tissue and / or cell into a human recipient. The invention also relates to a method of selecting a pig lacking an infectious porcine endogenous retrovirus, as well as their use for tissue and / or cell xenotransplantation into a human, as well as a method of gene editing of xenoantigens of selected pigs to further enhance donor organs, tissues and / or cells to avoid xenotransplantation rejection.

The invention belongs to the field of biology, and in particular relates to a method for constructing a monkey ITP model induced by a platelet-specific antigen. In particular, it provides a method for obtaining an ITP model by immunizing monkeys with CD61 protein as a specific antigen. Including the application of CD61 protein in the preparation of animal models of thrombocytopenic purpura, the sequence of the CD61 protein is shown in SEQ ID NO:2. The induction method of the invention can effectively induce thrombocytopenia and purpura symptoms, and obtain a stable monkey ITP model.

The invention discloses a TMV-CMV-PVY three-virus (tobacco mosaic virus, cucumber mosaic virus and potato virus) colloidal gold rapid test strip, which is composed of a sample pad, a colloidal gold pad, an NC membrane, a piece of water-absorbent filter paper and a backing, wherein a colloidal gold labeled TMV specific antibody, a CMV specific antibody and a PVY specific antibody are enveloped on the colloidal gold pad, and the three antibodies are secreted from hybridoma cell strains BALB / c 15 50, BALBc 15 27 and BALBc 15 8; a detection line and a control line are arranged on the NC membrane; specific antigens of three viruses are enveloped on the detection line; and a colloidal gold labeled second antibody is enveloped on the control line. The test strip is especially suitable for detecting the TMV, CMV and PVY in Guangdong tobacco-growing areas, and the test strip is rapid, sensitive, accurate, low in cost and convenient to operate; the test strip is capable of simultaneously diagnosing various viruses by conducting sampling once; the test strip is quite suitable for in-situ preliminary screening of a great batch of samples; and the test strip, in actual production, has a high application value, and the test strip has a good popularization and application prospect.

The invention relates to isolation, identification and application of a Fabricius bursa active hexapeptide for promoting an immunoreaction of avian influenza and / or a Newcastle disease bivalent vaccine. The Fabricius bursa active hexapeptide has an amino acid composition of Lys Gly Asn Arg Val Tyr, has a simple structure and is extremely weak in immunogenicity. The active peptide has the functionof promoting the immune response of the vaccine and can promote the antibody reaction against the AIV antigen and the antibody reaction against the NDV antigen in the mouse in the mouse immunization experiment. The active peptide can promote the cellular immune response, improve lymphocyte viability and an antigen presentation reaction and improve the vaccine immunopotency, can be used as a vaccine adjuvant or immunopotentiator in animal vaccine application research, can improve the immune response of animal organisms to specific antigens, can improve the immune efficacy of vaccines and can improve the ability of animal organisms resisting epidemic disease infection.

The invention relates to a whole blood interferon-gamma (INF-gamma) specific antigen protein prepared by a gene engineering method and a preparation method and application thereof, a nucleotide sequence for coding the protein, a recombinant vector and a recon. The antigen protein is a fusion protein obtained by connecting early secreting antigen target 6 (ESAT-6), cultufiltrate protein 10 (CFP-10) and MPB64 in any order through a connecting peptide, wherein the connecting peptide is a short peptide which does not influence the immunogenicity of the ESAT-6, the CFP-10 and the MPB64. The tuberculosis specific antigen quickly diagnoses tuberculosis, has high sensitivity and specificity, and has great significance for the early diagnosis of the tuberculosis.

The invention discloses a TMV-CMV (Tobacco Mosaic Virus-Cucumber Mosaic Virus) double-virus colloidal gold quick detection test strip which consists of a sample pad, a colloidal gold pad, an NC membrane, water absorption filter paper and a back lining, wherein the colloidal gold pad is coated with a TMV specific antibody and a CMV specific antibody which are marked by colloidal gold; the two antibodies are respectively generated by hybridoma cell strains BALB / c-15-50 and BALBc-15-27; a detection line and a control line are arranged on the NC membrane; the detection line is coated with specific antigens of two viruses; the control line is coated with a colloidal gold marked second antibody. The test strip is quick, sensitive, accurate, low in cost and easy and convenient to operate particularly for detection of TMV and CMV in Guangdong tobacco-growing areas; simultaneous diagnosis of the two viruses is realized by one-time sampling; the TMV-CMV double-virus colloidal gold quick detection test strip is very suitable for on-site preliminary screening of a large batch of samples and has application value to actual production and a good popularization and application prospect.