Construction method of platelet specific antigen induced monkey ITP model
A platelet and antigen technology, applied in animal husbandry and other directions
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Embodiment 1
[0016] Example 1: Induction of ITP animal model.
[0017] Experimental animals: cynomolgus monkeys (Cynomolgus), male or female, aged 2.5-4.0 years, weighing 2.5-4.5kg.
[0018] Experimental environment: The test monkeys will be placed in stainless steel monkey cages (800 mm × 700 mm × 820 mm) in Pengli Biological Animal Room. The adaptation period is: 2 monkeys per cage; the experimental period and postoperative care are: 1 (with equipped animal welfare appliances). The room number in which the animals were housed throughout the experiment will be recorded in the experiment record. The room where the test monkeys are placed is located in the Pengli biological animal room, and the number of filtered ventilation in this area is 10-20 times per hour. Temperatures will remain between 20-26°C (68-79°F) and relative humidity 40-70%. Continuously observe and record temperature and humidity. The lighting conditions were 12 hours (08:00-20:00) daylight irradiation and 12 hours no ...
Embodiment 3
[0023] Example 3: Detection of ITP animal models.
[0024] Detection Indicator:
[0025] 1) Body weight: before the start of the experiment, measure and record the body weight of the animal;
[0026] 2) Blood collection: before immunization, from 7 days after immunization, venous blood collection twice a week, EDTA anticoagulant, about 200ul, up to 28 days;
[0027] 3) Complete blood count: the frequency is the same as that of blood collection, no significant decrease in platelets was observed within three weeks, and the animals were given a second antigen immunization on Day 7-28, and the method was the same as the first time;
[0028] 4) Serum sample preparation: the frequency is the same as that of blood collection, the serum is separated to about 400ul, and stored at -80°C;
[0029] 5) Determination of bleeding time: several time points before immunization and when a significant decrease in platelets is found.
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