A method for detecting residual cells in apheresis platelet products

Abstract

This case involves a method for detecting residual cells in apheresis platelet products, including: step 1) taking a cell sample, adding staining antibody reagents to complete cell-specific labeling; step 2) flattening the cell sample, and observing it with a fluorescent microscope counting; wherein, the staining antibody reagents include: labeled CD41 and CD61 for staining platelets; labeled CD45 for staining leukocytes; and nuclear dyes for staining leukocyte nuclei. In this case, the simultaneous detection of residual white blood cells, red blood cells, and platelets can be realized by using the combination of cell staining and labeling and fluorescence imaging; later, the fluorescence microscope and software calculation can be adapted to realize automatic detection and counting; the operation is simple and the efficiency is high.

Description

technical field [0001] The invention relates to the detection field of platelet products, in particular to a method for detecting residual cells in apheresis platelet products. Background technique [0002] At present, platelet blood transfusion is one of the most important treatment methods for thrombocytopenia and various diseases, and has developed into the second most clinical component blood transfusion after red blood cell transfusion. However, ineffective platelet transfusions still occur frequently. Blood patients generally need multiple platelet transfusions. Ineffective transfusions are likely to occur after multiple transfusions of platelets. The main reason is the production of allotype antibodies. Studies have shown that, HLA antibody, HPA antibody and ABH antibody exist in most patients with ineffective platelet transfusion, and 80% of them have HLA antibody. The main reason for the antibody production is likely to be the stimulation of the patient's immune sy...

AI Technical Summary

Problems solved by technology

In recent years, although some blood banks have adopted more accurate flow cytometry analysis, the relative sample preparation of flow cytometry is cumbersome, has high requirements for operators, and is expensive, so it is not universally applicable

[0006] The current residual cell detection method has many problems and limitations

The manual counting method based on the large-capacity Nageotte blood cell counting disc has large errors, poor repeatability, and no original records, which do not meet modern requirements for record keeping and chain of evidence

[0007] Conventional blood cell counters on the market are difficult to accurately count relevant indicators in apheresis platelet products, especially residual white blood cells and red blood cells

The detection limit of commonly used cell counting methods (such as blood cell counter, flow cytometer, etc.) is generally at 10 4 cells / mL, it is impossible to quickly and effectively detect the residual cells in the whitened apheresis platelets

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