The invention discloses a Gemini-type ampholytic surfactant based on perfluorononene and perfluorohexene, and a preparation method thereof. The preparation method comprises the following steps: taking perfluorononene and perfluorohexene as the raw materials, carrying out phenol condensation reactions and chlorosulfonation reactions so as to obtain perfluoroolefineoxyl benzene sulfonyl chloride, then subjecting the perfluoroolefineoxyl benzene sulfonyl chloride to carry out condensation reactions with 2-dimethylamino ethylamine or 3-dimethylamino propylanmine so as to obtain perfluoroolefineoxyl benzene sulfonamide whose amine part comprises a tertiary amine group, then subjecting the perfluoroolefineoxyl benzene sulfonamide to carry out condensation reactions with 1,2-dihaloethane, 1,3-dihalopropane, 1,4-dihalobutane, 1,5-dihalopentane, 3-oxa-1,5-dihalopentane, or 1,3-dihaloacetone, wherein the halogen element is chlorine or bromine, and finally subjecting the reaction product to carry out condensation reactions with vinyl sulfonate, chloroethyl sulfonate, bromoethyl sulfonate or 1,3-propanesultone so as to obtain the Gemini-type ampholytic fluorine surfactant with excellent surface activity and low critical micelle concentration. The Gemini-type ampholytic fluorine surfactant has the prominent characteristics of simple synthetic operation, low cost, high surface activity, and low using concentration, and has a vast market prospect.