83 results about "Glyceraldehyde phosphate" patented technology

The invention provides a reagent for detecting a Notch signal path as well as a PCR (Polymerase Chain Reaction) detecting method and application thereof. The detecting reagent comprises PCR reaction primers for detecting an ADAM10 gene, an ADAM17 gene, an AES gene, a CBL gene, a CCND1gene, a CD44 gene, and the like, and primers of corresponding internal-reference regulatory genes GAPDH (Reduced Glyceraldehyde-Phosphate Dehydrogenase), ACTB (Beta-Actin) and B2M (Beta-2-Microglobulin). The invention provides the reagent for detecting core molecule changes of the NOTCH signal channel; by virtue of the detecting reagent, genes associated with the NOTCH channel can be quickly detected on transcriptional level by virtue of real-time fluorescent quantitative PCR; on this basis, core molecules and a mechanism of action of a tumor NOTCH signal channel are analyzed and researched, so that an NOTCH regulatory channel of tumor-associated medicaments is quickly and accurately found, and therefore, a powerful tool is provided for screening anti-tumor medicaments, discussing the mechanism of a novel targeted medicament, and the like.

The invention discloses a preparation method of a periodic wuchereria malayi compound multivalent DNA (deoxyribonucleic acid) vaccine. The preparation method comprises the following steps of: designing a primer, amplifying BmGAPDH (reduced glyceraldehyde-phosphate dehydrogenase) and BmCPI genes by PCR (polymerase chain reaction), purifying segments of BmGAPDH and BmCPI genes, connecting and converting a T carrier, screening and identifying an I positive recombinant clone, building and identifying an I recombinant expression vector, extracting and purifying a mass of recombinant plasmid and the like. The preparation method is convenient in preparation and good in effect. The periodic wuchereria malayi which is popular in China is taken as a research object, and the periodic wuchereria malayi cysteine proteinase inhibitor / 3-phosphoglyceraldehyde dehydrogenase eukaryotic expression recombinant plasmid is built. The test result proves that the compound multivalent DNA vaccine can induce the mouse to generate the specific immune response reaction, so that a satisfied effect can be obtained, and the periodic wuchereria malayi compound multivalent DNA vaccine can be successfully prepared.

The invention pertains to novel proteins corresponding to Chrysosporium glycosyl hydrolases of families 7 and 10, exhibiting a minimum aminoacid identity of 70 and 75%, respectively, with the amino acid sequence of SEQ ID No's 2 and 4, and to a protein corresponding to a Chrysosporium glyceraldehyde phosphate dehydrogenase, exhibiting at least 86% amino acid identity with the partial amino acid sequence of SEQ ID No. 6. The invention further relates to nucleic acid sequences encoding these proteins, and especially to promoter sequences regulating the expression of the corresponding genes. The preferred host for expressing these genes is a fungus, especially a Chrysosporium strain.

The invention relates to an ammonia diagnosing / measuring reagent box which utilizes the technique of the enzyme contrasting color method and the enzyme jointing method, and belongs to the technical field of the medicine / food / environment test. The main components of the reagent box of the invention mainly include cushion liquid, coenzyme, glutamic acid, adentosine triphosphoric acid, glyceraldehyde-3-phosphate, glutamine synthetase, glyceraldehyde-3-phosphate dehydrogenase and stabilizing agent; the reagent box generates a series of enzymic reactions through the mixing of the samples and the reagent at a certain cubage rate, and then the reactants are positioned under an ultraviolet / visible light analyzer which tests the reducing speed of the absorbency at the main wave length of 340nm, thereby measuring the concentration size of the enzyme. The invention can absolutely get needed measuring result through the ultraviolet / visible light analyzer.

According to the present invention, an inorganic phosphoric acid is detected by a method which includes: subjecting a sample to a measurement system containing glyceraldehyde-3-phosphate, oxidized nicotinamide adenine dinucleotide or oxidized nicotinamide adenine dinucleotide phosphate, glyceraldehyde phosphate dehydrogenase, and an electron mediator; and measuring a current value in the measurement system. In the method, a pyrophosphate is quantitatively measured with high sensitivity and at a high speed through converting the pyrophosphate in a sample into an inorganic phosphoric acid. Such a measurement of a pyrophosphate allows for quantitative determination of the pyrophosphate which is produced concurrent with the extension of a DNA, thereby enabling the detection of the presence of a targeted nucleic acid, and typing of a base in a SNP site of a targeted DNA.

The invention pertains to novel proteins corresponding to Chrysosporium glycosyl hydrolases of families 7 and 10, exhibiting a minimum aminoacid identity of 70 and 75%, respectively, with the amino acid sequence of SEQ ID No's 2 and 4, and to a protein corresponding to a Chrysosporium glyceraldehyde phosphate dehydrogenase, exhibiting at least 86% amino acid identity with the partial amino acid sequence of SEQ ID No. 6. The invention further relates to nucleic acid sequences encoding these proteins, and especially to promoter sequences regulating the expression of the corresponding genes. The preferred host for expressing these genes is a fungus, especially a Chrysosporium strain.

The invention relates to the fields of molecular biology and phymatology, and discloses an application of a biomarker associated with a colorectal cancer. The marker is PIK3C2G, and particularly, deoxyribonucleic acid (DNA) of the marker is inversely related to survival time after colorectal cancer occurrence and chemotherapy of a sufferer relative to copy number variation. The invention also relates to a primer pair, a kit and a method for detecting the PIK3C2G in a colorectal cancer, the primer pair for detecting the PIK3C2G and a primer pair for detecting a reference gene reduced glyceraldehyde-phosphate dehydrogenase(GAPDH) are contained in the kit of the PIK3C2G. An upstream primer base sequence is shown in SEQ ID NO:1. The primer can be applied to detection of the copy number variation of the PIK3C2G through polymerase chain reaction (PCR) amplification, so that detection of the colorectal cancer and evaluation of the curative effect of oxaliplatin (FOLFOX) chemotherapy of the colorectal cancer through the PIK3C2G become possible.

The present invention relates to an integrated protein C-terminal enrichment method, which comprises: selective dimethylation labeling, DL-glyceraldehyde-3-phosphate labeling and affinity removing with titania. According to the method, an enzymolysis product of a protein sample is subjected to selective N-terminal amino dimethylation labeling on a reverse phase trapping column under an acid condition, the side chain amino of the peptide segment is labeled on a DL-glyceraldehyde-3-phosphate column, and finally a TiO2 material is adopted to carry out affinity removing to remove the phosphate-labeled peptide segment so as to obtain the protein C-terminal, wherein sample transfer and desalination lyophilization are not required to be performed during the process. The method has advantages of high labeling efficiency, high selectivity, simple treatment steps, time saving, sample consumption saving, and sample loss and contamination reducing. In addition, the method provides good compatibility with the chromatography-mass spectrometry separation and identification platform.

The invention discloses a selection method of reference genes in quantitative real-time PCR analysis of jerusalem artichoke, and relates to the field of quantitative PCR. The method comprises the following steps: taking different tissues (radicles, immature stems, leaves, stem blocks and petals) of the jerusalem artichoke as materials, and carrying out expression analysis of the reference genes of18S ribosomal RNA gene (18S rRNA), transcription elongation factor gene (Ef-1a), actin gene (Actin and beta-actin), 3-glyceraldehyde phosphate dehydrogenase (GAPDH), 25S ribosomal RNA gene (25S rRNA)and poly-ubiquitin enzyme gene (UBQ)7 by using a q PCR technology; carrying out statistical assessment on the obtained data and analyzing expression change of all housekeeping genes by utilizing GeNorm and NormFinder software, so as to screen out relatively-stable genes as the reference genes of the jerusalem artichoke, which are used for studying the gene dosage changes of the jerusalem artichoke.

The invention provides an L-lactic acid catalysis reaction system and a preparation method of L-lactic acid. The L-lactic acid catalysis reaction system contains glucose dehydrogenase, dihydroxyacid anhydrase, 2-keto-3-deoxygluconate, glyceraldehyde phosphate dehydrogenase and L-lactic dehydrogenase. The L-lactic acid can be obtained without adding ATP (Adenosine Triphosphate) by performing a cascade enzymatic catalytic reaction in a reactor by utilizing five enzymes of the reaction system and taking glucose as a raw material.

A sensor of pyrophosphate which can detect pyrophosphate conveniently with high sensitivity in a method for measuring pyrophosphate in SNP typing utilizing a primer extension reaction is provided.A sensor of pyrophosphate which is characterized by including: an insulative substrate 1; an electrode system that is formed thereon and has a measurement electrode 2 and a counter electrode 3; and a plurality of reaction reagent layers that are provided on the substrate 1 and include pyrophosphatase, glyceraldehyde-3-phosphate dehydrogenase, diaphorase, glyceraldehyde-3-phosphate, oxidized nicotineamide adenine dinucleotide, an electronic mediator, a magnesium salt and a buffer component, reaction reagent layer 36 including the enzyme being separated from reaction reagent layer 35 including the buffer component, and reaction reagent layer 37 including glyceraldehyde-3-phosphate being separated from the reaction reagent layer 35 including the buffer component.

The invention provides a method for detecting salidroside synthetase gene expression activity by using dual reference genes. Specifically, a primer which is obtained through design and screening is adopted to amplify to obtain glyceraldehydes phosphate dehydrogenase and phytochelatin synthetase genes, and correct and detect the gene expression activity of three key enzymes, namely, tyrosine decarboxylase, tyrosine transaminase and phenylalanine ammonialyase of synthesized salidroside in rhodiola crenulata tissue culture seedlings by using the gene as the dual reference genes. The method comprises the following steps: extracting total RNA of a rhodiola crenulata plant; synthesizing a cDNA first chain; and by using a fluorescent quantitative PCR technique, amplifying by using a given primer so as to obtain the dual reference genes of GAPDH and PCS, and correcting and detecting the expression activity of synthesized salidroside key enzyme genes TyDC, TAT and PAL in the rhodiola crenulata tissue culture seedlings. The method has the advantages of rapidness, accuracy, practicability, stability and the like in aspects such as production quality control and product activity detection on rhodiola crenulata tissue culture seedlings, cell suspension culture, hairy root culture and the like.

The invention discloses a shikimic acid synthesis method. According to the method, fructose 6-phosphate and glyceraldehydes-3-phosphate ester serve as raw materials, and shikimic acid is generated under the catalytic function of various enzymes. The generation technology is simple in step, reagents are safe, byproducts are few in the reaction process, economic benefits are improved, the yield of the obtained shikimic acid is 50-86%, and the purity is 98.1-99.5%.

The invention provides a gene detection reagent kit for evaluating alcohol tolerance. The gene detection reagent kit comprises six amplification primers for three genes including an internal reference gene GAPDH (glyceraldehyde phosphate dehydrogenase), a gene ADH1B (ethanol dehydrogenase 1B) and a gene ALDH2 (acetaldehyde dehydrogenase 2) and five Taq-man probe primers. One of the Taq-man probe primers is positioned at the internal reference gene GAPDH and is used for carrying out FAM fluorescence labeling; two other Taq-man probe primers are SNP (single nucleotide polymorphism) probes positioned at the gene ADH1B and are used for carrying out VIC and ROX (roxithromycin) labeling; the remaining two Taq-man probe primers are SNP probes positioned at the gene ALDH2 and are used for Cy5 and Quasar705 labeling, BHQ is used as a quenching group, and delta ct values can be computed. The gene detection reagent kit has the advantages that the alcohol tolerance of healthy persons can be evaluated by the gene detection reagent kit from the aspect of genes, and accordingly the healthy persons can know the strength and the weakness of alcohol tolerance, can drink alcohol scientifically and can carry on occasion such as party, gathering and banquet related to alcohol culture.

The invention relates to a zinc diagnosis / determination reagent kit which adopts the techniques of enzymatic colorimetric method and enzyme couple reaction, belonging to the technical field of medicine / food / environment test and determination. The invention also relates to the method principle of determining the zinc concentration, and the reagent composition and components. The component of the reagent kit mainly comprises buffer solution, coenzyme, phosphate monoester, glyceraldehyde-3-phosphate, alkaline phosphatase, glyceraldehyde-3-phosphate dehydrogenase and stabilizer. The method for determining the zinc concentration is as follow: mixing the sample and the reagent according to a certain volume ratio to perform a series of enzymatic reactions; placing the reactant under an ultraviolet / visible light analyzer to detect the ascending speed of the absorbency at the main wavelength of 340 nm; finally determining the zinc concentration. The invention has the advantages that the required determining result is obtained only with the ultraviolet / visible light analyzer.

The invention discloses a screening method of reference genes in a skin tissue of sheep, which relates to the technical field of biological engineering. The screening method comprises the steps that: a method of the reference genes is confirmed; a given amount of RNA (ribonucleic acid) extract is taken and diluted by 2-10 times by ddH2O (double-distilled water); a 50 nanograms / microlitre RNA sample is taken to serve as a template, and a cDNA (complementary deoxyribonucleic acid) synthetic reagent kit is adopted for conducting inverse transcription to synthesize cDNA; the cDNA synthesized through the inverse transcription serves as a template, and six reference genes serve as primers; and finally, pairing variation analysis of normalized factors Vn / n+1 of the six reference genes are calculated based on a geNorm program, so as to decide the amount of the optimum reference genes. The screening method solves the problem that no screening method for GAPDH (reduced glyceraldehyde-phosphate dehydrogenase) genes in the skin tissue of a Chinese merino (Xinjiang type) serving as the reference genes is not available at present. Through the combination of the specificity of the SYBR Green I with double chain DNA to generate fluorescence, a real-time fluorescent quantitative PCR (polymerase chain reaction) detection method of the SYBR Green I for the GAPDH genes in the skin tissue of the Chinese merino (Sinkiang type) is established.

The invention relates to a preparation method of 4-phosphoric acid erythrose, comprising the following steps: immobilizing heat-resistant transketolase at the temperature of 55-65 DEG C; and then taking 6-fructose phosphate and 3-glyceraldehyde phosphate as raw materials to prepare the 4-phosphoric acid erythrose by enzymatic reaction. In the preparation method, the heat-resistant and specifically transformed transketolase of the 4-phosphoric acid erythrose is prepared by adopting a molecular biology technology, and the 6-fructose phosphate and the 3-glyceraldehyde phosphate are taken as the raw materials to efficiently prepare the 4-phosphoric acid erythrose by adopting an immobilized enzyme technology. The preparation method is simple and practical.

The invention relates to a kit for identifying the genetype of a silky fowl skin color by detecting EDN3 (endothelin 3) gene copy number difference. The kit comprises a reference gene GAPDH (reduced glyceraldehyde-phosphate dehydrogenase) amplification primer (1), a target gene EDN3 amplification primer (2), calibration sample DNA (deoxyribonucleic acid) (3), fluorescent quantitative PCR (Polymerase Chain Reaction) reaction liquid (4) and double distilled water (5). The kit is characterized in that real-time fluorescent quantitative PCR reaction is carried out on a target gene EDN3 and a reference gene GAPDH by using the DNA of a tested sample as a template, Ct values are respectively recorded by using the calibration sample DNA as a reference, the EDN3 gene copy numbers of the tested sample are obtained by calculation, and further the Fm genetype of the silky fowl skin color is judged according to the EDN3 gene copy number difference. The operation of the kit does not need the establishment of a standard curve; the kit is simple, convenient, fast, good in repeatability and high in detection efficiency; and through chicken breed test-crossing testing, the method can accurately reflect the Fm homozygosis and heterozygosis of the skin color of the individual silky fowl. The detection method is conductive to acceleration of screening of homozygosity of the silky fowl skin color, guides target breeding of high-quality silky fowl new variety lines and provides reliable germplasm guarantee for follow-up commercialization mating hybridization.

The invention relates to a method for synthesizing acrylic acid from cyanobacteria. The method comprises the following steps: 1), firstly, based on an NSI gene of cyanobacteria Syn7942, constructing an integrated vector NSI, and recombining to obtain a vector NSI-ceaS2; 2), transforming the recombinant vector NSI-ceaS2 into cyanobacteria Syn7942 cells, and then preliminarily screening out monoclonal transgenic cyanobacteria Syn7942 through a chloramphenicol solid culture medium; 3), transferring the screened monoclonal transgenic cyanobacteria Syn7942 to a liquid culture medium with chloramphenicol resistance, and after the cyanobacteria grow out, extracting a cyanobacteria genome for PCR verification of a target gene; 4), transferring the successfully verified monoclonal transgenic cyanobacteria Syn7942 to the liquid culture medium for culture, and when the cyanobacteria grow till OD730 is greater than or equal to 1, adding IPTG for inducing expression of a gene ceaS2, and catalytically synthesizing the acrylic acid by an enzyme by using glyceraldehyde-3-phosphate (G3P) or dihydroxyacetone phosphate (DHAP) produced by photosynthesis of the cyanobacteria as a substrate; 5), performing separation and purification. By the method, when the acrylic acid is synthesized through transformation of the cyanobacteria as substrate organisms, only sunlight and moisture and the like are required as production raw materials, production equipment and a production environment are easy to construct, and high electricity consumption is not required, so that the production cost of the methodprovided by the invention is greatly lower than those of other production methods.

A pyrophosphoric acid sensor that in the method of measuring pyrophosphoric acid in SNP typing making use of primer extension reaction, realizes convenient detection of pyrophosphoric acid with high sensitivity. There is provided a pyrophosphoric acid sensor composed of insulating substrate (1); formed thereon, an electrode group consisting of measuring electrode (2) and counter electrode (3); and superimposed on the substrate (1), multiple reaction reagent layers consisting of pyrophosphatase, glyceraldehyde-3-phosphate dehydrogenase, diaphorase, glyceraldehyde-3-phosphate, oxidized nicotinamide adenine dinucleotide, electron mediator, magnesium salt and buffer solution components wherein reaction reagent layer (35) containing buffer solution components is separated from enzyme-containing reaction reagent layer (36), characterized in that reaction reagent layer (37) containing glyceraldehyde-3-phosphate is separated from the reaction reagent layer (35) containing buffer solution components.

The invention discloses a method for extracting and detecting IncRNA in gastric juice. The method comprises the following steps: gastric juice extraction, gastric juice pretreatment, trichloromethane extraction, isopropanol participation, ethanol washing, drying a ribonucleic acid (RNA), RNA reverse transcription, polymerase chain reaction (PCR) detection and the like. Compared with the prior art, the method has the advantages that the impurities such as polysaccharide, mucoprotein and the like in the gastric juice are removed by the steps of gastric juice pretreatment, trichloromethane extraction, isopropanol participation, ethanol washing, drying a ribonucleic acid, RNA reverse transcription, and PCR detection. High-quality and high-yield extraction of the IncRNA in the gastric juice is achieved on the basis of simplifying experimental operation steps; fluorescent quantitative RT-PCR detection of the target IncRNA is achieved; reduced glyceraldehyde-phosphate dehydrogenase (GAPDH) is found out to be a good exterior parameter gene in the gastric juice by Ct value analysis of the GAPDH on 130 cases of gastric samples; a GAPDH reference Ct value range for judging the total RNA mass of the gastric juice is also proposed on the basis; and the method has great significance on further research of the occurrence regularity of IncRNA in the gastric juice and the relationship between the occurrence regularity of IncRNA in the gastric juice and the physiological status and the disease.

The invention provides an application of a gene IKZF3 in preparing a lung cancer diagnosis kit and the kit. The kit is composed of five parts, including a Real-Time PCR (Polymerase Chain Reaction) primer, a DNA (Desoxvribose Nucleic Acid) standard substance, an RNA (Ribose Nucleic Acid) extraction system, an RNA reverse transcription system and a Real-Time PCR system. The kit is used for analyzing the tumor tissue of a clinical lung cancer patient, detecting the relative expression quantity of the target gene IKZF3 relative to a reference gene GAPDH (reduced glyceraldehyde-phosphate dehydrogenase) in a sample and thus judging the prognosis of the patient.

The invention discloses a new way for synthesizing acetyl coenzyme A and a derivative product thereof using glycolic aldehyde. The method comprises reaction that glycolic aldehyde reacts with 3-glyceraldehyde phosphate under enzyme catalysis to generate 5-phosphoric acid arabinose, wherein the enzyme is selected from aldolase, transaldolase and isozyme thereof and mutate enzyme. The method is relatively high in catalytic rate; the theoretical yield of carbon of a reaction route is 100%; the carbon loss avoided; G3P, an enzyme and a coenzyme all can be recycled; the reaction efficiency is relatively high; and the cost is reduced. In addition, the coenzymes RpiA and Rpe from a pentose phosphate pathway are high in affinity with a substrate and high in catalytic activity. According to the method, more obvious advantages are developed in the production processes, of in vitro continuous polyenzyme catalysis, fed-batch fermentation and continuous fermentation, capable of controlling the substrate level.

A sensor of pyrophosphate which can detect pyrophosphate conveniently with high sensitivity in a method for measuring pyrophosphate in SNP typing utilizing a primer extension reaction is provided.A sensor of pyrophosphate which is characterized by including: an insulative substrate 1; an electrode system that is formed thereon and has a measurement electrode 2 and a counter electrode 3; and a plurality of reaction reagent layers that are provided on the substrate 1 and include pyrophosphatase, glyceraldehyde-3-phosphate dehydrogenase, diaphorase, glyceraldehyde-3-phosphate, oxidized nicotineamide adenine dinucleotide, an electronic mediator, a magnesium salt and a buffer component, reaction reagent layer 36 including the enzyme being separated from reaction reagent layer 35 including the buffer component, and reaction reagent layer 37 including glyceraldehyde-3-phosphate being separated from the reaction reagent layer 35 including the buffer component.

The invention discloses a stably expressed reference gene group of harmonia axyridis with different factors and application of the reference gene group. Specifically, a scheme of a stably expressed reference gene group of harmonia axyridis with different factors is that a stably expressed reference gene group under different temperature conditions comprises 18S, 28S and GAPDH (Reduced Glyceraldehyde-Phosphate Dehydrogenase), comprises 18S, 28S and HSP90 under different light cycle conditions, comprises 18S, 28S and HSP70 at different age stages, and comprises 18S, 28S and RP49 at different tissue combinations. By adopting the reference gene group, a most stable reference gene group of harmonia axyridis under four factors is confirmed for a first time, reference gene selection bases are made for study on gene expression levels of different tissues of different ages of harmonia axyridis under different temperature conditions and different light cycle conditions, and a solid base is madefor studying gene functions of the harmonia axyridis, RNAi (Ribonucleic Acid Interfere) transgenic crop environment risk estimation, and the like.

The invention discloses a preparation method of a printing and dyeing finishing agent used for fabric. The preparation method includes the steps that 1, sodium sulfate, coconut oil die thanolamide, 3-glyceraldehydes phosphate dehydrogenase, palmitic acid and sodium oleate are taken and added into pure water to prepare a water solution; 2, sulphate of ricinoleic acid butyl ester, polyethylene glycol dilaurate, aluminum oxide, titanium dioxide and p-hydroxyphenylacetyl chloride are added into glycerin, and a glycerin solution is prepared at 40-60 DEG C; 3, the water solution and the glycerin solution are mixed, polysorbate 80 and glycerin monostearate are added, and then the printing and dyeing finishing agent is obtained through stirring. Compared with the prior art, the preparation method is simple in technology and low in cost, and the prepared printing and dyeing finishing agent used for the fabric can obviously improve the tensile property and abrasion resistance of the fabric and be suitable for different kinds of fabric, and is wide in application range and excellent in property.

The invention discloses a kit and a method for measuring the content of 1,3-diphosphoglycerate based on a micromethod. A reagent of the kit comprises an extracting solution, a first reagent and a second reagent, wherein the extracting solution is prepared from Tris-HCl, EGTA, mercaptoethanol and glycerinum in a mixed mode, the first reagent is prepared from MgSO4.7H2O, NADH, ATP and glyceraldehyde-3-phosphate dehydrogenase in a mixed mode, and the second reagent is prepared from Tris-HCl. The method comprises the principle that the glyceraldehyde-3-phosphate dehydrogenase can reversely catalyze the 1,3-diphosphoglycerate and the NADH to generate glyceraldehyde-3-phosphate, inorganic phosphorus and NAD, the glyceraldehyde-3-phosphate dehydrogenase and the NADH are added into a reaction system, and by measuring the reduction amount of the NADH at 340 nm, the content of the 1,3-diphosphoglycerate can be reflected. The kit and the method for measuring the content of the 1,3-diphosphoglycerate have the characteristics that the application range is wide, operation is easy and convenient, the detection time is short, detection sensitivity is high, the cost is low, repeatability is good, and the accuracy rate is high.

The invention discloses a method for extracting and detecting IncRNA in gastric juice. The method comprises the following steps: gastric juice extraction, gastric juice pretreatment, trichloromethane extraction, isopropanol participation, ethanol washing, drying a ribonucleic acid (RNA), RNA reverse transcription, polymerase chain reaction (PCR) detection and the like. Compared with the prior art, the method has the advantages that the impurities such as polysaccharide, mucoprotein and the like in the gastric juice are removed by the steps of gastric juice pretreatment, trichloromethane extraction, isopropanol participation, ethanol washing, drying a ribonucleic acid, RNA reverse transcription, and PCR detection. High-quality and high-yield extraction of the IncRNA in the gastric juice is achieved on the basis of simplifying experimental operation steps; fluorescent quantitative RT-PCR detection of the target IncRNA is achieved; reduced glyceraldehyde-phosphate dehydrogenase (GAPDH) is found out to be a good exterior parameter gene in the gastric juice by Ct value analysis of the GAPDH on 130 cases of gastric samples; a GAPDH reference Ct value range for judging the total RNA mass of the gastric juice is also proposed on the basis; and the method has great significance on further research of the occurrence regularity of IncRNA in the gastric juice and the relationship between the occurrence regularity of IncRNA in the gastric juice and the physiological status and the disease.

The invention relates to a kit for diagnosing / mensurating carbon dioxide by utilizing the technologies of the enzymic colorimetric method and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the carbon dioxide, and belongs to the technology field of medical / food / environmental inspection and measurement. The main components of the kit include a buffer solution, coenzyme, phosphoenolpyruvic acid, glyceraldehyde-3-phosphate, glyceraldehyde-3-phosphate dehydrogenase, phosphoenol pyruvate carboxylase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, an enzymatic reactions occurs, then the reactant is placed under an ultraviolet / visible light analyzer, and the degree / velocity of the increase in absorbance at 340nm of the dominant wavelength is detected, thereby mensurating the concentration of the carbon dioxide.