The invention relates to a method for synthesizing
acrylic acid from
cyanobacteria. The method comprises the following steps: 1), firstly, based on an NSI
gene of
cyanobacteria Syn7942, constructing an integrated vector NSI, and recombining to obtain a vector NSI-ceaS2; 2), transforming the recombinant vector NSI-ceaS2 into
cyanobacteria Syn7942 cells, and then preliminarily screening out
monoclonal transgenic cyanobacteria Syn7942 through a
chloramphenicol solid culture medium; 3), transferring the screened
monoclonal transgenic cyanobacteria Syn7942 to a
liquid culture medium with
chloramphenicol resistance, and after the cyanobacteria grow out, extracting a cyanobacteria
genome for PCR
verification of a
target gene; 4), transferring the successfully verified
monoclonal transgenic cyanobacteria Syn7942 to the
liquid culture medium for culture, and when the cyanobacteria grow till OD730 is greater than or equal to 1, adding IPTG for inducing expression of a
gene ceaS2, and catalytically synthesizing the
acrylic acid by an
enzyme by using
glyceraldehyde-3-
phosphate (G3P) or
dihydroxyacetone phosphate (DHAP) produced by
photosynthesis of the cyanobacteria as a substrate; 5), performing separation and purification. By the method, when the
acrylic acid is synthesized through transformation of the cyanobacteria as substrate organisms, only
sunlight and
moisture and the like are required as production raw materials, production equipment and a production environment are easy to construct, and high
electricity consumption is not required, so that the production cost of the methodprovided by the invention is greatly lower than those of other production methods.