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Shikimic acid synthesis method

A synthetic method and technology of shikimic acid, applied in the direction of fermentation, etc., to achieve the effect of simple steps, less by-products, and improved economic benefits

Active Publication Date: 2017-06-30
SUZHOU DUMEI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the synthesis of shikimic acid has made great progress, a variety of reagents need to be used in the synthesis process and the control of the reaction conditions is relatively strict. After the reaction, the treatment of by-products and waste liquid also requires more financial resources.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A synthetic method of shikimic acid, the method steps are as follows:

[0043] Put the fructose 6-phosphate and glyceraldehyde-3-phosphate into the reactor at a weight ratio of 2.5:1, the added amount of glucose is 30g / L, and the added amount of the yeast extract is 10g / L, add The production strain contains transketolase, DAHP synthase, 3-dehydrocolchicine synthase, shikimate dehydrogenase and glucose dehydrogenase; the reaction control temperature is 60℃; adding H 3 PO 3 And NH 4 OH adjusts the pH to 7.3; the reaction time is 28h.

[0044] The yield of shikimic acid obtained was 71%, and the purity was 98.1%.

Embodiment 2

[0046] Put the fructose 6-phosphate and glyceraldehyde-3-phosphate into the reactor according to the weight ratio of 4:1, the added amount of glucose is 100g / L, the added amount of the yeast extract is 20g / L, add The production strain contains transketolase, DAHP synthase, 3-dehydrocolchicine synthase, shikimate dehydrogenase and glucose dehydrogenase; the reaction control temperature is 25℃; adding H 3 PO 3 And NH 4 OH adjusts the pH to 6.7; the reaction time is 36h.

[0047] The yield of shikimic acid obtained was 50%, and the purity was 99.5%.

Embodiment 3

[0049] Put the fructose 6-phosphate and glyceraldehyde-3-phosphate into the reactor in a weight ratio of 3:1, the added amount of glucose is 25g / L, and the added amount of the yeast extract is 17g / L, add The production strain contains transketolase, DAHP synthase, 3-dehydrocolchicine synthase, shikimate dehydrogenase and glucose dehydrogenase; the reaction control temperature is 20℃; adding H 3 PO 3 And NH 4 OH adjusts the pH to 7.0; the reaction time is 30h.

[0050] The yield of the obtained shikimic acid was 81% and the purity was 99.3%.

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Abstract

The invention discloses a shikimic acid synthesis method. According to the method, fructose 6-phosphate and glyceraldehydes-3-phosphate ester serve as raw materials, and shikimic acid is generated under the catalytic function of various enzymes. The generation technology is simple in step, reagents are safe, byproducts are few in the reaction process, economic benefits are improved, the yield of the obtained shikimic acid is 50-86%, and the purity is 98.1-99.5%.

Description

Technical field [0001] The invention relates to the technical field of chemical synthesis, in particular to a method for synthesizing shikimic acid. Background technique [0002] Shikimic acid is an organic compound isolated from Japanese "mangcao" in 1885. It was later discovered that it also exists in star anise, tomato leaves, hypericum and other plants. Among them, it is used in traditional Chinese medicine "star anise". The highest content. The molecular formula of shikimic acid is C 7 H 10 O 5 , The structural formula is as follows: [0003] [0004] Shikimic acid has strong physiological activity. It is a highly selective enzyme inhibitor and a good antibacterial and antiviral agent. Shikimic acid can affect the metabolism of arachidonic acid, inhibit platelet aggregation, inhibit the formation of arterial and venous thrombosis and cerebral thrombosis, and has a good anti-inflammatory and analgesic effect. The early preparation of shikimic acid was mostly made from star a...

Claims

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Application Information

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IPC IPC(8): C12P7/42
CPCC12P7/42
Inventor 陈车翘
Owner SUZHOU DUMEI BIOTECH CO LTD
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