31 results about "Fructose 6-phosphate" patented technology

A topical skin rejuvenation preparation to relieve wrinkles, increase the skin's natural production of hyaluronic acid, reverse the lack of suppleness, hydrate from within, erase spider veins, reduce varicose veins, lighten aging dark blotches (“liver spots”/Lentigos, Senile Lentigines), decrease acne, and reduce under eye puffiness includes Glucosamine (2-amino-2-deoxy-alpha-D-glucose), a hexosamine (6 carbon amino sugar), including its derivative and precursor compounds: Glucosamine Sulfate, Glucosamine Hydrochloride, Glucose-6-Phosphate, Acetyl Glucosamine, Fructose-6-phosphate, Glucosamine-6-Phosphate, to increase production of Hyaluronic acid and collagen from Glucosamine Sulfate, its precursors and derivatives and to increase skin muscle tone by Dimethylaminoethanol (DMAE) while over coming deficiency it creates in each cell's production of PhosphatidylCholine, whose deficiency damages cell membranes, as well as mitochondrial and lysosome membranes.

The invention discloses recombinant corynebacterium glutamicum for accumulating N-acetylneuraminic acid and application thereof and belongs to the field of genetic engineering. The recombinant corynebacterium glutamicum takes corynebacterium glutamicum as an expression host, and an N-acetylneuraminic acid synthesis way is reinforced through over-expression of a glucosamine-fructose-6 phosphate aminotransferase gene, a glucosamine acetylase encoding gene, a phosphatase encoding gene, an acetylglucosamine isomerase encoding agene and an N-acetylneuraminic acid synthase encoding gene; an N-acetylneuraminic acid transport protein encoding gene in the corynebacterium glutamicum and a related gene in an intracellular N-acetylneuraminic acid decomposition, utilization and metabolism are knocked off to obtain corynebacterium glutamicum genetically engineered bacteria for extracellularly accumulating the N-acetylneuraminic acid and the yield reaches 110mg/L; a foundation is laid for further modifying corynebacterium glutamicum through metabolic engineering to produce the N-acetylneuraminic acid.

The invention relates to a method for measuring 1,5-anhydroglucitol by oxidase, which can be widely applied in the field of medical and biochemical technologies. The method comprises the following steps of: converting glucose (Glu) in a sample into fructose-6-phosphate (Fru-6-P) by glucokinase (GK) and phosphoglucose isomerase (PGI), and establishing an ATP regeneration system by pyruvate kanise (PK) and phosphoenolpyruvate (PEP) in the system so as to ensure that Glu is converted into Fru-6-P. In the sample, 1,5-AG reacts in the presence of pyranose oxidase (PROD) to produce 1,5-fructosan (1,5-AF) and H2O2, and then H2O2 is colored and quantified via a Trinder's system..

The invention discloses a shikimic acid synthesis method. According to the method, fructose 6-phosphate and glyceraldehydes-3-phosphate ester serve as raw materials, and shikimic acid is generated under the catalytic function of various enzymes. The generation technology is simple in step, reagents are safe, byproducts are few in the reaction process, economic benefits are improved, the yield of the obtained shikimic acid is 50-86%, and the purity is 98.1-99.5%.

The invention provides a kit for detecting glucose-6-phosphate isomerase and a use method of the kit, and belongs to the technical field of enzyme detection. The kit comprises a first reagent and a second reagent, wherein the first reagent uses Tris buffer as a solvent and comprises adenosine triphosphate, beta-nicotinamide adenine dinucleotide trihydrate, a protective agent and a preservative; the second reagent uses the Tris buffer as the solvent and comprises magnesium chloride, fructose-6-phosphate, glucose-6-phosphate dehydrogenase, a stabilizer and the preservative; and the concentrationof the Tris buffer is 50-500 mmol/L. The kit provided by the invention can obtain a result of detecting glucose-6-phosphate isomerase at 6 min 20 s to 9 min 40 s, thereby shortening detection time.

Cerebral ischemia-reperfusion injury may be treated by compounds that increase brain O-GlcNAcylation, such as a therapeutic amount of a compound that increases the hexosamine biosynthesis pathway flux that bypasses glutamine/fructose-6-phosphate amidotransferase 2 or a therapeutic amount of a compound that inhibits OGA. The initial and transient elevation of brain O-GlcNAcylation is neuroprotective and helps ameliorate cerebral ischemia-reperfusion injury when administered within three hours of the ischemia-reperfusion-induced brain injury and continues for at least two days.

Disclosed is a method of preparing pure or substantially pure D-myo-inositol-3-phosphate from glucose-6-phosphate and/or fructose-6-phosphate. The method may also be applied to protected and/or derivative forms of glucose-6-phosphate and/or fructose-6-phosphate so as to form protected/derivative forms of D-myo-inositol-3-phosphate, for use in further chemical reactions. The enzyme D-myo-inositol-3-phosphate synthase (INO1) is contacted with the glucose-6-phosphate and/or fructose-6-phosphate to generate labeled or unlabeled, protected or unprotected D-myo-inositol-3-phosphate, which may be further reacted and/or purified.

This invention relates to glutamine:fructose-6-phosphate amidotransferase, or its partial peptide or a salt thereof; a DNA coding for the protein; a recombinant vector; a transformant; a method for producing the protein; a pharmaceutical composition comprising the protein, its partial peptide or a salt thereof; and an antibody against the protein or its partial peptide. The protein, its partial peptide or a salt thereof, and the DNA are useful for a prophylactic or therapeutic agent for hypoglycemia. The antibody can be used in the assay of the protein, its partial peptide or a salt thereof. The protein, its partial peptide or a salt thereof is useful as a reagent for the screening for candidate medical compounds.

The present invention relates to plant cells and plants which synthesize an increased amount of hyaluronan, and to methods for preparing such plants, and also to methods for preparing hyaluronan with the aid of these plant cells or plants. Here, plant cells or genetically modified plants according to the invention have hyaluronan synthase activity and additionally an increased glutamine:fructose 6-phosphate amidotransferase (GFAT) activity compared to wild-type plant cells or wild-type plants. The present invention furthermore relates to the use of plants having increased hyaluronan synthesis for preparing hyaluronan and food or feedstuff containing hyaluronan.

The invention discloses a new special solid complex enzyme (ALD, TIM and GDH) reagent for determining the content of fructose diphosphate sodium (FDP) and a preparation method and an application method thereof. The solid complex enzyme comprises aldolase (ALD), triosephosphate isomerase (TIM) and glycerol phosphate dehydrogenase (GDH). In the invention, the solid complex enzyme reagent containing the aldolase (ALD), the triosephosphate isomerase (TIM) and the glycerol phosphate dehydrogenase (GDH) is extracted from animal muscles as main raw materials under a certain condition. The solid complex enzyme can determine the content of the fructose diphosphate sodium under a certain condition, and has the characteristics of strong specificity (the solid complex enzyme reacts with only the fructose diphosphate sodium and does not react with glucose, fructose, glucose 6-phosphate or fructose 6-phosphate), good repeatability (RSD=1.45%, n=6), higher accuracy and simple and convenient operation.

An isolated nucleic acid molecule is provided comprising a nucleotide sequence which encodes a glutamine:fructose-6-phosphate amidotransferase from E. coli which is particularly suitable for expression in cotton plant cells. The invention also relates to plant cells or plants, in particular to cotton plant cells or cotton plants which produce an increased amount of positively charged polysaccharides in their cell walls. Furthermore methods and means are provided to increase the content of positively charged polysaccharides in the cell walls of cotton cells, in particular in cotton fibers. Fibers obtained from such cotton plants have an altered chemical reactivity which can be used to attach reactive dyes or other textile finish reagents to these fibers.

The invention relates to a protein with enzymatic activity, comprising a GFAT sequence and at least one sequence of a purification marker, the sequence for the purification marker being inserted between two consecutive amino acids of the GFAT sequence.