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Kit for detecting glucose-6-phosphate isomerase and use method of kit

A phosphate isomerase and kit technology, applied in the field of enzyme detection, can solve the problems of complex program operation and long detection time.

Inactive Publication Date: 2019-04-09
浙江达美生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, mainly the Elisa antigen antibody is used to detect the content of glucose 6 phosphate isomerase, and the diagnostic kit is made by adopting the immunolabeling and adsorption technology antigen-antibody binding reaction principle, but the kit requires complex procedures, such as antibody Preparation, labeling of antibodies, preparation of antibody-coated polystyrene microwell plates, it takes a long time to get test results

Method used

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  • Kit for detecting glucose-6-phosphate isomerase and use method of kit
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  • Kit for detecting glucose-6-phosphate isomerase and use method of kit

Examples

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Effect test

Embodiment 1

[0055] A kit for detecting glucose-6-phosphate isomerase, the kit includes reagent 1 and reagent 2;

[0056] Reagent 1 uses double distilled water as solvent and contains:

[0057] Tris 100mmol / L, adenosine triphosphate 2mmol / L, oxidized coenzyme I 5mmol / L, polyethylene glycol p-isooctyl phenyl ether 20g / L, ascorbic acid 0.005g / L and sodium azide 1g / L.

[0058] Reagent 2 uses double distilled water as solvent and contains:

[0059] Tris 100mmol / L, anhydrous magnesium chloride 2mmol / L, fructose-6-phosphate 100mmol / L, glucose-6-phosphate dehydrogenase 15KU / L, bovine serum albumin 5g / L and sodium azide 1g / L.

Embodiment 2

[0061] A kit for detecting glucose-6-phosphate isomerase, the kit includes reagent 1 and reagent 2;

[0062] Reagent 1 uses double distilled water as solvent and contains:

[0063] Tris 150mmol / L, adenosine triphosphate 12mmol / L, oxidized coenzyme I 8mmol / L, polyoxyethylene lauryl ether 1g / L, glutathione 0.1g / L and PC3000.5g / L.

[0064] Reagent 2 uses double distilled water as solvent and contains:

[0065] Tris 150mmol / L, anhydrous magnesium chloride 5mmol / L, fructose-6-phosphate 60mmol / L, glucose-6-phosphate dehydrogenase 12KU / L, polyethylene glycol 20g / L, trehalose 45g / L and PC3000.5g / L.

Embodiment 3

[0067] A kit for detecting glucose-6-phosphate isomerase, the kit includes reagent 1 and reagent 2;

[0068] Reagent 1 uses double distilled water as solvent and contains:

[0069] Tris 200mmol / L, adenosine triphosphate 8mmol / L, oxidized coenzyme I 12mmol / L, polyethanol monolaurate 5g / L, glutathione 0.1g / L and sodium azide 2g / L.

[0070] Reagent 2 uses double distilled water as solvent and contains:

[0071] Tris 200mmol / L, anhydrous magnesium chloride 5mmol / L, fructose-6-phosphate 85mmol / L, glucose-6-phosphate dehydrogenase 12.5KU / L, mannitol 20g / L, trehalose 45g / L and sodium azide 1g / L.

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Abstract

The invention provides a kit for detecting glucose-6-phosphate isomerase and a use method of the kit, and belongs to the technical field of enzyme detection. The kit comprises a first reagent and a second reagent, wherein the first reagent uses Tris buffer as a solvent and comprises adenosine triphosphate, beta-nicotinamide adenine dinucleotide trihydrate, a protective agent and a preservative; the second reagent uses the Tris buffer as the solvent and comprises magnesium chloride, fructose-6-phosphate, glucose-6-phosphate dehydrogenase, a stabilizer and the preservative; and the concentrationof the Tris buffer is 50-500 mmol / L. The kit provided by the invention can obtain a result of detecting glucose-6-phosphate isomerase at 6 min 20 s to 9 min 40 s, thereby shortening detection time.

Description

technical field [0001] The invention relates to the technical field of enzyme detection, in particular to a kit for detecting glucose-6-phosphate isomerase and a method for using the kit. Background technique [0002] Glucose-6-phosphate isomerase (GPI; EC 5.3.1.9) is a kind of natural protein with multifunctional biological activities, which is involved in the pentose phosphate pathway of sugar metabolism and glycolysis, Catalyze the interconversion of glucose 6-phosphate (G 6-P) and fructose-6-phosphate (F-6-P), thereby catalyzing the isomerization of F-6-P into G 6-P. PGI has multiple functions: In the cell, PGI plays a key role in glycolysis and gluconeogenesis; outside the cell, PGI (also known as autocrine motor factor, Autocrine Motility Factor (AMF)) acts as a cytokine to stimulate cells Movement, and is associated with tumorigenesis and metastasis. [0003] In the prior art, mainly the Elisa antigen antibody is used to detect the content of glucose 6 phosphate is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/533C12Q1/32
CPCC12Q1/32C12Q1/533
Inventor 方朝君金立方郑江萍丁佳
Owner 浙江达美生物技术有限公司
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