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Cotton fibers with increased glucosamine content

a technology of glucosamine and cotton fibers, which is applied in the direction of enzymology, yarn, transferases, etc., can solve the problems of not possessing the chemical versatility of cotton fibers and not enduring well washing

Inactive Publication Date: 2017-03-16
BASF AGRICULTURAL SOLUTIONS SEED LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patent is about a type of DNA expression that occurs in plant fibers, which are also called “fiber cells” or“fibers”. The expression of this DNA is higher in fibers than in other tissues of the plant, preferably about 2 to 10 times higher. This suggests that the DNA is likely to be actively expressed in fibers for a specific purpose.

Problems solved by technology

However, like other natural cellulose containing fibers, cotton fibers do not possess the chemical versatility of synthetic fibers, due to the relative inert nature of the β-1-4 linked glucose monomers in cellulose.
Direct dyes are easier to use and less expensive than fiber-reactive dyes, but do not withstand well washing.

Method used

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  • Cotton fibers with increased glucosamine content
  • Cotton fibers with increased glucosamine content

Examples

Experimental program
Comparison scheme
Effect test

example 1

Construction of a Chimeric Gene Encoding a Glutamine:Fructose-6-Phosphate-Amidotransferase (GFAT) Protein for Expression in Cotton Cells

[0119]A DNA molecule having the nucleic acid sequence according to SEQ ID 1 was synthesized by Entelechon GmbH. The nucleotide sequence was designed i) to encode a polypeptide according to SEQ ID 2 and ii) to optimize the nucleotide sequence for expression in cotton plant cells. For this purpose, factors such as codon usage, mRNA secondary structure, the AT content, cryptic splice sites or restriction sites were taken into account.

[0120]The resulting nucleotide sequence as disclosed in SEQ ID 1 is 75% identical (1390 matching bases out of 1830) to the published nucleotide sequence encoding a GFAT protein from E. coli which was adapted to the codon usage in plants (WO 2007 / 039314).

[0121]Using standard recombinant DNA techniques, the following chimeric GFAT gene was constructed: A chimeric glutamine-6-phosphate-amidotransferase gene comprising the fol...

example 2

Generation of Transgenic Cotton Plants Expressing a Glutamine: Fructose-6-Phosphate Amidotransferase

[0148]The T-DNA vectors were introduced into Agrobacterium tumefaciens strains containing a helper Ti-plasmid and used in cotton transformation essentially as described in WO00 / 71733. T0 plants were further analyzed as described in Example 3.

example 3

Determination of the Glucosamine Content of Cotton Fibers

[0149]Fibers from transgenic cotton T0 plants were isolated, treated with trifluoroacetic acid (TFA) to hydrolyze the glucosamine polymers and analyzed for the glucosamine content by HPLC. All steps were carried out following standard protocols.

[0150]Fibers of untransformed lines contained about 0.01% of GlcN. The results for the measured glucosamine content of cotton fibers from different T0 plants expressing the GFAT gene according to the invention under the control of the SCW-PRP promotor (transformed with pTDBI 252) are depicted in Table 3.

TABLE 3GlcN content of cotton fibers from individual T0 plantsGFAT optimized (pTDBI 252)GFAT control (pTGK 110)pl1 0.0115 *cpl1 0.0101 *pl2 0.0118 *cpl2 0.0112 *pl3 0.0136 *cpl3 0.0120 *pl4 0.0141 *cpl4 0.0121 *pl50.0318cpl5 0.0125 *pl60.0340cpl60.0316pl70.0371cpl70.0330pl80.0389cpl80.0334pl90.0401cpl90.0349pl100.0405cpl100.0425pl110.0431cpl110.0446pl120.0448cpl120.0536pl130.0472cpl130.0...

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Abstract

An isolated nucleic acid molecule is provided comprising a nucleotide sequence which encodes a glutamine:fructose-6-phosphate amidotransferase from E. coli which is particularly suitable for expression in cotton plant cells. The invention also relates to plant cells or plants, in particular to cotton plant cells or cotton plants which produce an increased amount of positively charged polysaccharides in their cell walls. Furthermore methods and means are provided to increase the content of positively charged polysaccharides in the cell walls of cotton cells, in particular in cotton fibers. Fibers obtained from such cotton plants have an altered chemical reactivity which can be used to attach reactive dyes or other textile finish reagents to these fibers.

Description

FIELD OF THE INVENTION[0001]The present invention relates to the modification of the chemical reactivity of cotton fibers. In particular, the present invention provides cotton fibers comprising positively charged oligosaccharides such as oligo-N-acetylglucosamines or oligo-glucosamines. Due to the amino groups these fibers have a modified reactivity which can be exploited for attaching other substances to the fibers to alter their characteristics. Such substances can e.g. be reactive dyes or other reactants such as flame retardants, water, oil and soil repellents, anticrease agents, softeners, antistatic agents, fluorescent whitening agents etc.[0002]The current invention provides methods and means to increase the efficiency of production of glucosamine oligomers in cotton plant cells such as fiber cells.BACKGROUND OF THE INVENTION[0003]Cotton fiber is the single most important textile worldwide. About 80 million acres of cotton are harvested annually across the globe. Cotton is the...

Claims

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Application Information

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IPC IPC(8): C12N15/82D02G3/02C12N9/10
CPCC12N15/8246C12N9/1096D02G3/02C12N15/8223C12Y206/01016
Inventor MEULEWAETER, FRANK
Owner BASF AGRICULTURAL SOLUTIONS SEED LLC
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