Application of PIK3C2G in evaluation and detection kit for curative effect of colorectal cancer chemotherapy
A technology for colorectal cancer and kits, applied in the fields of molecular biology and tumor biology, able to solve problems such as possible and specific applications that have not been studied
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
specific Embodiment
[0022] Collection of samples and their follow-up information
[0023] The inventor has collected colorectal cancer patient samples from Ruijin Hospital since 2006, and selected 92 samples (35 cases with a survival period of less than five years, and 57 cases with a survival period of more than five years) of stage III colorectal cancer tumors after data collation and follow-up. Paraffin embedded tissue samples.
[0024] Paraffin sample DNA extraction
[0025] Cut 5 mg of sample from paraffin-embedded tissue, put it into a 2 ml centrifuge tube, add 1 ml of xylene, and vortex for 10 seconds. Centrifuge at room temperature for 2 minutes at 14,000 rpm. Discard the supernatant. Add 1 ml of absolute ethanol to wash the precipitate, vortex, centrifuge at 14,000 rpm for 2 minutes at room temperature, and discard the supernatant. Place the centrifuge tube in an ultra-clean bench to remove excess ethanol. Add 180 μl RTL buffer (Qiagen), 20 μl proteinase K, mix well, incubate at 56°...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com