The invention belongs to the technical field of bacteria detection, and more specifically discloses a group B streptococcus fluorescent quantitative PCR detection kit. The group B streptococcus fluorescent quantitative PCR detection kit comprises a group of group B streptococcus fluorescent quantitative PCR detection primers and probes; the primers and probes comprise GBS amplification primer pairGBS-F and GBS-R, GBS detection probe GBS-P, internal reference primer pair beta-actin-F and beta-actin-R, and internal reference probe beta-actin-P; the nucleotide sequences of GBS-F, GBS-R, GBS-P, beta-actin-F, beta-actin-R, and beta-actin-P are represented by SEQ ID NO:1-6 respectively. The group B streptococcus fluorescent quantitative PCR detection kit can be used for detecting GBS in samplewith complex composition, and detecting single copy templates, is wider in linearity range, is capable of avoiding false positive results caused by amplification products pollution, and is high in sensitivity and specificity.