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Method for evaluating developmental potential of oocyte by utilizing relative expression of GRIM19 in granulosa cell

A technology of GRIM-19 and relative expression, which is applied in biochemical equipment and methods, measurement/testing of microorganisms, DNA/RNA fragments, etc., to achieve the effects of easy operation, reduction of adverse environmental stimuli, and accurate developmental potential

Active Publication Date: 2016-08-17
SHANDONG UNIV QILU HOSPITAL
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Problems solved by technology

At present, there is no relevant research report on using the relative expression of GRIM-19 in granulosa cells to judge the developmental potential of oocytes

Method used

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  • Method for evaluating developmental potential of oocyte by utilizing relative expression of GRIM19 in granulosa cell
  • Method for evaluating developmental potential of oocyte by utilizing relative expression of GRIM19 in granulosa cell
  • Method for evaluating developmental potential of oocyte by utilizing relative expression of GRIM19 in granulosa cell

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Embodiment 1

[0044] A method for evaluating the developmental potential of oocytes by using the relative expression of GRIM-19 in granulosa cells, wherein the relative expression of GRIM-19 in the granulosa cells refers to GRIM-19mRNA and internal reference β-actin (β- action) ratio indicates:

[0045] When the GRIM-19 / β-action value in the granulosa cells of oocytes used for fertilization is 0.16-0.35, the obtained oocytes are judged to be oocytes with high developmental potential, and the granulosa cells of oocytes used for fertilization Among them, when the GRIM-19 / β-action value is 0-0.12, the obtained oocytes are judged to be oocytes with low developmental potential.

[0046] In this example, the relative expression of GRIM-19 in granulosa cells was measured by the following steps:

[0047] 1. Collection of human granulosa cells:

[0048] Conventional ovulation induction plan, mainly long or short ovulation induction treatment, is used to monitor the size of follicles by B-ultrasoun...

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Abstract

The invention discloses a method for evaluating developmental potential of an oocyte by utilizing the relative expression of GRIM-19 in a granulosa cell. When the relative expression of the GRIM-19 in the granulosa cell of the oocyte is 0.16-0.35, the obtained oocyte is judged to be high in developmental potential; when the relative expression of the GRIM-19 is 0-0.12, the obtained oocyte is judged to be low in developmental potential; a beta-actin gene is selected for internal reference. The method disclosed by the invention is a more objective, accurate and quantizable oocyte quality evaluation method, is noninvasive without repeatedly taking out an incubator for observation, so that the adverse environmental stimulation is reduced. Due to being quantizable, the method disclosed by the invention has a specific numerical range, is easy to operate and solves the problem that due to artificial subjective factors, the judgment standards are not unified.

Description

technical field [0001] The invention relates to a method for evaluating the developmental potential of oocytes by using the relative expression level of GRIM-19 in granulosa cells. Background technique [0002] In recent years, problems such as accelerated pace of life, intensified job competition, deteriorating environmental pollution, and delayed marriage and childbearing age brought about by social development have gradually become prominent, and the incidence of infertility has increased significantly every year. In-vitro fertilization-embryo transfer (in-vitro fertilization, IVF-ET) and intra-cytoplasmic sperm injection (ICSI) technologies are the key technologies used in the treatment of infertile patients. In the process of IVF / ICSI, accurate assessment of the developmental potential of oocytes and embryos after fertilization is the focus of modern assisted reproductive technology. At present, the methods used by reproductive centers at home and abroad to evaluate th...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 晁岚杨阳邓晓惠程来洋蒋利刚沈彦军
Owner SHANDONG UNIV QILU HOSPITAL
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