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Zinc diagnosis/measuring reagent kit and method for measuring zinc concentration

A technology of reagents and stabilizers, applied in the field of medical/food/environmental inspection and determination, can solve the problems of expensive instruments

Inactive Publication Date: 2008-05-07
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is much simpler to operate than the colorimetric method and the fluorescence method, but the instrument is expensive, and it is recommended as the method for zinc determination because it can analyze the zinc content most accurately

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The zinc diagnosis / measurement reagent of the present embodiment is a single reagent, comprising:

[0046] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0047] Stabilizer 50mmol / L

[0048] Coenzyme 3mmol / L

[0049] Phosphate monoester 20mmol / L

[0050] Glyceraldehyde-3-phosphate 3mmol / L

[0051] Alkaline phosphatase 20000U / L

[0052] Glyceraldehyde-3-phosphate dehydrogenase 12000U / L

[0053] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0054] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the tested zinc sample to the reagent is 1 / 25, the reaction The direction is positive reaction (rising reaction), the delay time is about 1 minute, and the detection time is...

Embodiment 2

[0057] The zinc diagnosis / measurement reagent of the present embodiment is a double reagent, comprising:

[0058] Reagent 1

[0059] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0060] Stabilizer 50mmol / L

[0061]Coenzyme 3mmol / L

[0062] Phosphate monoester 10mmol / L

[0063] Glyceraldehyde-3-phosphate 6mmol / L

[0064] Reagent 2

[0065] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0066] Stabilizer 50mmol / L

[0067] Alkaline phosphatase 30000U / L

[0068] Glyceraldehyde-3-phosphate dehydrogenase 18000U / L

[0069] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0070] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the tested zinc sample to reagent 1 and reagent 2 is 2 / 20 / 5, the reac...

Embodiment 3

[0073] The zinc diagnosis / measurement reagent of the present embodiment is three reagents, comprising:

[0074] Reagent 1

[0075] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0076] Stabilizer 50mmol / L

[0077] Coenzyme 3mmol / L

[0078] Glyceraldehyde-3-phosphate 9mmol / L

[0079] Reagent 2

[0080] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0081] Phosphate monoester 20mmol / L

[0082] Reagent 3

[0083] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0084] Stabilizer 50mmol / L

[0085] Alkaline phosphatase 50000U / L

[0086] Glyceraldehyde-3-phosphate dehydrogenase 32000U / L

[0087] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0088] When measuring zinc concentration, set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 3...

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Abstract

The invention relates to a zinc diagnosis / determination reagent kit which adopts the techniques of enzymatic colorimetric method and enzyme couple reaction, belonging to the technical field of medicine / food / environment test and determination. The invention also relates to the method principle of determining the zinc concentration, and the reagent composition and components. The component of the reagent kit mainly comprises buffer solution, coenzyme, phosphate monoester, glyceraldehyde-3-phosphate, alkaline phosphatase, glyceraldehyde-3-phosphate dehydrogenase and stabilizer. The method for determining the zinc concentration is as follow: mixing the sample and the reagent according to a certain volume ratio to perform a series of enzymatic reactions; placing the reactant under an ultraviolet / visible light analyzer to detect the ascending speed of the absorbency at the main wavelength of 340 nm; finally determining the zinc concentration. The invention has the advantages that the required determining result is obtained only with the ultraviolet / visible light analyzer.

Description

technical field [0001] The invention relates to a zinc diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring zinc concentration, which belongs to the technical field of medicine / food / environmental inspection and determination. Background technique [0002] Zinc is related to the growth and development of organisms. Zinc has important physiological functions, nutritional effects and clinical diagnosis and treatment significance. Determination of serum zinc and Cu / Zn ratio has important practical value for evaluating the zinc nutritional status of the body and for the diagnosis and treatment of many diseases. [0003] The determination methods of zinc include complexometric titration, fluorescence photometry, colorimetry, polarography, atomic absorption spectrophotometry, anodic stripping voltammetry and neutron activation method. [0004] Complexometric titration is time-consuming, requires a large amount of samples, and has l...

Claims

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Application Information

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IPC IPC(8): G01N33/84G01N21/78G01N21/25C12Q1/32C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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