80 results about "Glucaric Acid" patented technology

The present invention generally relates to processes for the chemocatalytic conversion of a glucose source to an adipic acid product. The present invention includes processes for the conversion of glucose to an adipic acid product via glucaric acid or derivatives thereof. The present invention also includes processes comprising catalytic oxidation of glucose to glucaric acid or derivative thereof and processes comprising the catalytic hydrodeoxygenation of glucaric acid or derivatives thereof to an adipic acid product. The present invention also includes products produced from adipic acid product and processes for the production thereof from such adipic acid product.

The invention discloses a preparation method of gluconic acid and glucaric acid in the technical field of organic electrochemistry synthesis. The preparation method comprises the steps that an electro-catalytic membrane serving as an anode and an auxiliary electrode serving as a cathode are connected with a voltage-stabilized source by wires to form an electro-catalytic membrane reactor; by taking a glucose aqueous solution as a reactant, and an inorganic substance as an electrolyte, gluconic acid and glucaric acid are prepared by catalytic oxidation of glucose with the electro-catalytic membrane under the action of an electric field; membrane penetration liquid is collected by suction of a peristaltic pump; obtained target products are separated from reaction liquid in real time by permeation of the membrane; the membrane penetration liquid is separated and purified to form gluconic acid and glucaric acid products; and yields of gluconic acid and glucaric acid are adjusted and controlled by adjusting and controlling current density and membrane flux. The preparation method integrates catalytic oxidation and separation functions, and can separate the obtained target products from the reaction liquid timely, so that the continuous high efficiency of the electro-catalytic membrane is maintained effectively; a side reaction is avoided; and the selectivity of the target products is improved.

The invention relates to a dual-hydrophilic thermo-sensitive polymer/lactic acid polymer composite nanofiber felt and a preparation method thereof. The nanofiber felt comprises the following components: dual-hydrophilic thermo-sensitive polymer and lactic acid polymer with the mass ratio of 10:1. The preparation method comprises the following steps: (1) carrying out free radical polymerization reaction on glucaric acid divinyl ester and N-isopropyl acrylamide to obtain the dual-hydrophilic thermo-sensitive polymer; (2) dissolving the high polymer in a solvent, stirring for 1-4 hours, and carrying out ultrasonic degassing to obtain a spinning solution A; (3) dissolving the lactic acid polymer in the solvent, stirring for 1-8 hours, carrying out ultrasonic degassing to obtain a spinning solution B; and (4) mixing the spinning solution A with the spinning solution B, and carrying out electrostatic spinning to obtain the dual-hydrophilic thermo-sensitive polymer/lactic acid polymer composite nanofiber felt. According to the invention, the method is continuously feasible, the operation is simple and easy, industrialization is easy to realize, and the obtained nanofiber felt can be used for carrying medicines and control the release of the medicine; and the nanofiber felt can also be implanted into a release system and can be used in the fields of tissue engineering scaffold and the like.

The present disclosure relates to a method for producing glucaric acid, and more particularly, to a method for producing glucaric acid including the steps of: (1) inputting aldohexose and potassium hydroxide to an aqueous solution; and (2) deriving a catalytic oxidation reaction after adding a supported noble metal catalyst to the aqueous solution under the presence of oxygen gas.

The invention discloses a method for producing glucaric acid by improving yeast fermentation by use of fusion expression and belongs to the field of metabolic engineering. According to the method, a myoinositol oxygenase (MIOX) and aldehyde acid dehydrogenase (Udh) are fused and then cloned and expressed in a yeast strain by virtue of direct fusion and addition of different connecting peptides, and therefore, the synthesis way of the glucaric acid is strengthened. By comparison, the yield of the Pichia pastoris GS115-Udh-(GS)1-MIOX is the highest; during shake-flask fermentation culture, 944.67mg/L of glucaric acid is generated in a YPD-MI (adding 10g/L myoinositol to a YPD culture medium) culture medium. During 3L fermentation tank culture, the yield of the glucaric acid can be 6.02g/L. According to the method, the policy of the metabolic engineering is adopted and a microbiological strain is modified to synthesize the target product glucaric acid; and as a result, a firm foundation is laid for efficient production of the glucaric acid by use of the biological method.

The present invention provides methods for producing a product of one or more enzymatic pathways. The pathways used in the methods of the invention involve one or more conversion steps such as, for example, an enzymatic conversion of guluronic acid into D-glucarate (Step 7); an enzymatic conversion of 5-ketogluconate (5-KGA) into L-Iduronic acid (Step 15); an enzymatic conversion of L-Iduronic acid into Idaric acid Step 7b); and an enzymatic conversion of 5-ketocluconate into 4,6-dihydroxy 2,5-diketo hexanoate (2,5-DDH) (Step 16). In some embodiments the methods of the invention produce 2,5-furandicarboxylic acid (FDCA) as a product. The methods include both enzymatic and chemical conversions as steps. Various pathways are also provided for converting glucose into 5-dehdyro-4-deoxy-glucarate (DDG), and for converting glucose into 2,5-furandicarboxylic acid (FDCA). The methods also involve the use of engineered enzymes that perform reactions with high specificity and efficiency. Additional products that can be produce include metabolic products such as, but not limited to, guluronic acid, L-iduronic acid, idaric acid, glucaric acid. Any of the products can be produced using glucose as a substrate or using any intermediate in any of the methods or pathways of the invention.

The invention discloses a method for preparing glucaric acid from inositol by adopting a ''one pot-two step'' enzyme catalysis means, and comprises the following steps: catalyzing inositol by utilizing inositol oxidase so as to produce an intermediate glucuronic acid; and then, converting the intermediate glucuronic acid into the glucaric acid by utilizing aldehyde dehydrogenase. Compared with existing gluconic acid production methods, the method disclosed by the invention has the advantages of being simple in production process, green, friendly, high in yield, conducive to large-scale preparation and the like.

The invention discloses an inositol oxygenase mutant and application thereof, and belongs to the technical field of bioengineering. The method comprises the steps that an inositol oxygenase mutant with improved enzyme activity is obtained through site-specific mutagenesis and co-expressed with aldehyde dehydrogenase (udh) in yeast, and the glucaric acid synthetic route is enhanced. Through comparisons, the yield of recombinant Pichia pastoris GS115-udh-mioxK59V/R171S is the highest, and glucaric acid 1.20g/L is produced in a YPD-MI culture medium during flask shaking fermentation culture. A metabolic engineering strategy is adopted, microbiological strains are improved to synthesize the target product glucaric acid, and a certain foundation is laid for efficient production of glucaric acid through a biological method.

The invention discloses a preparation method of glucaric acid and uronic acid dehydrogenase-NADH oxidase and an application thereof in preparation. The preparation method includes: taking xylan extracted in wood fiber plants as a raw material; preparing glucuronic acid by adopting a high-temperature xylanase and alpha-glucuronidase hydrolyzing technology; preparing the glucaric acid by oxidizing the glucuronic acid generated through the double-functional uronic acid dehydrogenase-NADH oxidase. With the method, use amount of NAD can be effectively saved, the NAD is regenerated in situ by taking the NADN oxidase as uronic acid dehydrogenase to promote circulation of coenzyme, the uronic acid dehydrogenase continuously catalyzes the glucuronic acid to be converted into the glucaric acid, catalytic efficiency is improved, and conversion rate can be up to 100%; meanwhile, the preparation method has the advantages of high efficiency, rapidness, specificity and moderate reaction conditions, and chemical substances of acid, alkali and the like are not involved in the entire preparation process; the preparation method is low in energy consumption and small in pollution, and operation is simple and easy to control.

The invention relates to a preparation method of calcium glucarate, and belongs to the field of pharmacy. The invention provides a preparation method of the calcium glucarate. The preparation method comprises the following steps: (1) putting glucose, oxygen and a metal catalyst palladium vanadium ammonium into a high-pressure reactor for catalytic oxidation reaction to obtain glucaric acid; (2) after the oxidation reaction, adding potassium-containing alkali to convert the glucaric acid into potassium gluconate; (3) enabling the potassium gluconate to react with an acid to release the glucaricacid; (4) enabling the glucaric acid to react with the calcium-containing alkali to obtain the calcium glucarate. The preparation method disclosed by the invention is pollution-free to the environment, and meanwhile, the total yield is increased; the catalyst can be repeatedly used; the cost is reduced; the calcium content is 98 to 102 percent; a small amount of industrial wastewater, waste residues and waste gas are generated; the preparation method is suitable for industrial production.

The invention discloses a nickel-hydrogen battery anode material prepared from glucaric acid metal complex doped beta-Ni(OH)2, comprising an anode active substance, electric conductive agent and adhesive, wherein the anode active substance is formed by the glucaric acid metal complex and the beta-Ni(OH)2 at the mass percent of 1.5-13.5 percent. The method for preparing the nickel-hydrogen battery anode material comprises the following steps: synthetizing glucaric acid metal complex powder; uniformly mixing a glucaric acid metal complex and beta-Ni(OH)2 under the basic condition by a solution method so as to prepare anode active substance powder; uniformly mixing the anode active substance powder, the electric conductive agent and the adhesive to form powder slurry; and filling the powder slurry into a foam nickel base plate and pressing and drying the powder slurry to prepare a battery anode. The material has high integrated electrical properties, and a nickel-hydrogen battery prepared by the invention is improved in the capacity by 1 percent to 7 percent and the utilization ratio, the cycle life and the charging efficiency in a high temperature environment.

The invention, which belongs to the technical field of bioengineering, discloses a method for improving the efficiency of synthesizing glucaric acid by a saccharomyces cerevisiae engineering strain. An opi1 deletion strain under the background of saccharomyces cerevisiae BY4741 is used as an original strain, myoinositol oxygenase MIOX4 derived from Arabidopsis thaliana and uronic acid dehydrogenase udh derived from pseudomonas syringae are linked through different linkers; and then integration to genome of a saccharomyces cerevisiae opi1 deletion strain is performed. After fusion expression, under the same fermentation condition, the maximum yield of the Bga-L12 engineering bacteria reaches 1.45 g/L, and the yield of the Bga-L12 engineering bacteria is increased by about 5 times by being compared with that of saccharomyces cerevisiae engineering bacteria which are not subjected to MIOX4 and udh fusion expression. And a foundation is laid for further metabolic transformation of the glucaric acid engineering bacteria.

The invention pertains to the use of C₂-C₆alpha-hydroxy acids selected from L-3-phenyl lactic acid, mucic (galactaric) acid, gluconic acid, glucaric acid, glyceric acid, 2-hydroxy butyric acid, alpha-hydroxyisovaleric acid, alpha-hydroxyisocaproic acid and erythronic acid and combinations thereof, as a growth- and production promoting ingredient, in culture media for culturing eukaryotic cells. The invention further pertains to culture media containing these alpha-hydroxy acid derivatives at levels of at least 0.001 mg/l.

A process is provided for making esters of FDCA, in which an aqueous feed comprising glucaric acid is first reacted with a high boiling first alcohol in the presence of an acid catalyst and with removing water during the reaction, to form a first product mixture comprising a first ester of FDCA and the high boiling first alcohol, then unreacted high boiling first alcohol is removed from the first product mixture. The first ester of FDCA and the high boiling first alcohol is then transesterified with a lower boiling second alcohol selected from the group consisting of methanol, ethanol, isopropanol and n-propanol, to form a second product mixture comprising a second ester of FDCA with the lower boiling second alcohol, and the second ester of FDCA with the lower boiling second alcohol is recovered.