36 results about "Genus Xanthomonas" patented technology

The invention relates to a mixed bacterial agent for reducing viscosity of thick oil, as well as a preparation method and a use thereof, belonging to the technical field of crude oil recovery. The mixed bacterial agent for reducing the viscosity of the thick oil comprises xanthomonas, pseudomonas, microbacterium and bacillus, and the volume ratio of fermentation solutions of all bacterial genera is (20-100):(40-180):(30-100):(10-80). The preparation method comprises the following steps: respectively fermenting and mixing first-stage seed solutions of all bacteria: (1) getting strains from sources: extracting the strains out of water of Bohai sea gulf, and taking thick crude oil as a carbon source for acclimation and screening; (2) preparing the first-stage seed solutions: respectively activating on a peptone-yeast slant culture medium and transferring into a liquid peptone-yeast culture medium for shaking and activation; and (3) preparing a fermentation solution of mixed bacteria: respectively transferring the first-stage seed solutions of the strains into a fermentation culture medium for culture, wherein the living bacteria count in the mixed bacteria is 10<8>-10<10> bacteria / ml. The mixed bacterial agent for reducing the viscosity of the thick oil is used for reducing the viscosity of the crude oil. The mixed bacterial agent for reducing the viscosity of the thick oil, disclosed by the invention, has the advantages of high activity, rapid growth, no secondary pollution, good viscosity-reducing effect and the like.

Provided are methods for growing and shipping sprouts and microgreens in the same container, growing while in shipment using moisture provided in a water-absorbent layer, with optional added beneficials, and including methods for producing sprouts and microgreens for consumption, and for pharmaceutical / nutriceutical use, comprising growth of sprouts in retail-ready containers, the container comprising a moisture-retaining layer of agar media or the like providing water for growth and obviating the need for irrigation during sprout growth. In certain aspects, media is supplemented with beneficial organisms or additives such as probiotic microbes, vitamins (e.g., B12), cofactors, nutrients, and other items (e.g., phytochemicals, natural colors, and antioxidants) which promote the growth of the beneficial microbes on the product, and / or which become incorporated into the product. In certain aspects, added beneficial microorganisms are selected to compete antagonize human pathogens such as Listeria, Salmonella, enterohaemorrhagic E. coli, Yersinia, and / or spoilage organisms (e.g., Erwinia, Pseudomonas and Xanthomonas).

In order to provide a method for culturing an immunopotentiator-containing organism having the experience of being eaten, inexpensively without requiring usage of a component derived from an animal, Acetobacter, Gluconobacter, Xanthomonas, Zymomonas or Enterobacter which is an edible gram-negative bacterium having an immunopotentiation function is cultured using a culture solution composed mainly of wheat or bean curd refuse. Thereby, Acetobacter can be obtained inexpensively and safely, and also a low molecular weight lipopolysaccharide which is the immunopotentiator can be obtained inexpensively and safely. Furthermore, no impurity derived from animal components is mixed.

The present invention provides plants, plant cells and methods of making transgenic plants or plant cells, wherein the plants or cells are resistant to bacterial blight. The invention provides isolated nucleic acid sequences comprising SEQ ID NO: 1 or fragments thereof which provide a plant with resistance to bacterial blight disease when transfected into the plant.

Substantially pure glycosidases capable for cleaving selected glycosidic bonds have been described including glycosidases isolated from Xanthomonas and recombinant glycosidases. Substrate specificity of isolated enzymes have been identified for GlcNacβ1-x, Galα1-3R, Galα1-6R, Galβ1-3R, Fucα-2R, Fucα1-3R, Fucα1-4R, Manα1-2R, Manα1-3R, Manα1-6R, Manβ1-4R, Xylβ1-2R, Glcβ1-4R, and Galβ1-4R providing improved capability for selectively cleaving a glycosidic linkage in a carbohydrate substrate and for forming modified carbohydrates.

The invention discloses an application method for an agent used to control vegetable bacterial diseases, and belongs to the field of chemical protection of plants. The agent is characterized in that the active ingredient is 2-Br-2-nitro-1, 3-propylene glycol, and by the way that the agent with an effective concentration of 1000 micrograms / ml is sprayed over whole plants, vegetable bacterial diseases can be controlled effectively. The agent has obvious effects of prevention and treatment on vegetable bacterial diseases caused by Pseudomonas Acidovorax, Xanthomonas, Erwinia, Clavibacter, and provides new technical supports for controlling the occurrence and development of vegetable bacterial diseases during production.

Methods and compositions for making citrus plants with enhanced resistance to Asiatic citrus canker (ACC) and other forms of citrus canker caused by Xanthonomas are provided. The methods involve transforming citrus plant cells with polynucleotide constructs comprising a promoter operably linked to nucleotide sequence that encodes a protein that is capable of triggering cell death in a citrus plant. The promoters of the invention are inducible by one or more Xanthomonas strains that cause citrus canker. Isolated nucleic acid molecules and expression cassettes comprising such polynucleotide constructs and promoters are further provided. Citrus plants with enhanced resistance to citrus canker are also provided.

An efficient bioherbiciding xanthomonas campestris pv. retroflexus is prepared through sampling the soil near the root of weeds such as crabgrass herb, caper euphorbia, cassia, etc and their stem andleaves, separating with NPC culture medium and chlorella pyrenoidosa and glutamine synthetase depressants, and screening the bacterial strains with high herbiciding activity and broad spectrum. Its advantage is high herbiciding effect.

The invention relates to a mixed microbial preparation for recovering soil polluted by composite pollutant oil in a biology manner and a manufacture method of the mixed microbial preparation. The mixed microbial preparation is made by mixing six bacterial strains including acinetobacter calcoaceticus, klebsiella pneumonia, enterobacter cancerogenus, pseudomonas stutzeri, rhodococcus erythropolis, and pseudomonas putida with one of rhodococcus, acinetobacter, gordonia, sphingobacterium, novosphingobium capsulatum, acinetobacter, and xanthomonas at a ratio of 1:1:2:2:1:1:1:1 and is used for recovering oil-polluted soil.

The invention relates to an application of a host defense inducer in preventing and treating selected bacteria pests in useful plants, wherein the selected bacteria pests are selected from acidovoraxavenae, burkholderia species, burkholderia glumae, phloem bacillus species, corynebacterium, erwinia species, pseudomonas syringae, pathogenic variant of pseudomonas syringa kiwi, pathogenic variant of pseudomonas syringe soybean, pathogenic variant of pseudomonas syringe tomato, pathogenic variant of pseudomonas syringe cucumber, streptomyces species, xanthomonas species, xanthomonas axonopodis,pathogenic variant of xanthomonas axonopodis citrus, pathogenic variant of xanthomonas axonopodis soybean, xanthomonas campestris, pathogenic variant of xanthomonas campestris banana, pathogenic variant of xanthomonas campestris peaches and plums, strawberry xanthomonas and semi-transparent xanthomonas. The invention also relates to a method for preventing and treating selected bacteria pests in useful plants by adopting the host defense inducer for treatment.

The present disclosure relates to substituted thiophene carboxamides and analogues thereof of formula (II) that may be used for protecting plants from bacterial diseases, in particular from bacterial diseases caused by bacteria belonging to the genus Xanthomonas.

The present invention relates to the use of dithiine-tetracarboximides of formula (I) for controlling selected bacterial harmful organisms in useful plants, wherein the bacterial harmful organisms are selected from the group consisting of Acidovorax avenae, Burkholderia spec., Burkholderia glumae, Candidatus Liberibacter spec., Candidatus Liberibacter asiaticus, Corynebacterium, Erwinia spec. (Dickeya, Pectobacterium carotovorum, Erwinia amylovora), Pseudomonas syringae, Pseudomonas syringae pv. actinidae, Pseudomonas syringae pv. glycinea, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. lachrymans, Pseudomonas tumefaciens (=Agrobacterium tumefaciens), Streptomyces spp., Xanthomonas spp., Xanthomonas ampelina, Xanthomonas axonopodis, Xanthomonas axonopodis pv. citri, Xanthomonas axonopodis pv. glycines, Xanthomonas campestris, Xanthomonas campestris pv. musacearum, Xanthomonas campestris pv. pruni, Xanthomonas campestris pv. Viticola, Xanthomonas fragariae and Xanthomonas transluscens or Xylella fastidiosa. The present invention also relates to a method for controlling the selected bacterial harmful organisms in useful plants by treatment with a dithiine-tetracarboximides of formula (I).

The invention provides a compound bactericide containing bronopol and amino-oligosaccharin and a preparation method thereof, the mass ratio of bronopol to amino-oligosaccharin is 25-60: 0.4-0.8. The bromonol and the aminooligosaccharide have synergistic effect within a certain compound proportion range, have chemical protection effect on crops in the actual use process, and obviously improve the prevention and control effect of the crops on bacterial diseases compared with single dose alone, the compound bactericide has wide sterilization range, is used for prevention and control of melon bacterial gummy stem blight, field melon bacterial angular leaf spot and most bacteria and fungi on vegetables, and has obvious prevention and treatment effects on pseudomonas, acidovorax, xanthomonas, erwinia and coryneform bacteria.

The present invention provides methods for development of a virulent bacteriophage-based treatment for the control of plant diseases caused by Xylella fastidiosa. The invention further provides methods of isolating and propagating bacteriophage virulent to X. fastidiosa in a Xanthomonas bacterial host and for treating or reducing symptoms of X. fastidiosa infection in a plant. The invention further provides methods of isolating and propagating bacteriophage virulent to Xanthomonas axonopodis pv. citri and for treating or reducing symptoms of Xanthomonas axonopodis pv. citri infection in a plant.

The present disclosure relates to substituted thiophene carboxamides and analogues thereof of formula (I) that may be used for protecting plants from bacterial diseases, in particular from bacterial diseases caused by bacteria belonging to the genus Xanthomonas.

The invention discloses an artificial flora cellulose biotransformation method. An artificial flora is composed of Clostridium, Ureibacillus and Pseudoxanthomonas, and the colony formation unit numberratio is (3-70):(10-50):9. Organic products comprise ethanol, acetic acid, acetone, butyric acid, butanol and the like and can serve as biofuel or chemical raw materials. Experiments prove that the artificial flora conversion rate, the raw material conversion rate, the cellulose degradation capacity and the organic product total output are significantly increased, and the raw material conversionrate reaches up to 0.473 g / g substrate. The method is easy to operate, the cost is low, and the method has popularization and application value.

The present invention presents a method for the aerobic production of xanthan by bacteria of the genus Xanthomonas on a solid or semi-solid substrate. In the exemplary embodiment of the method, a substrate is provided that has a total solids content of about 6.5% or higher. The substrate is sterilized and cooled. Bacteria of the genus Xanthomonas are inoculated into the substrate and incubated. After incubation, the bacteria are destroyed, the substrate is either diluted or washed, and the xanthan is isolated.

The purpose of the present invention is to provide a vaccine composition which is safe and effective as a preventive medicine or a treatment medicine for infections and / or cancer, can effectively induce a systemic immune response and a mucosal immune response, and which is capable of being administered to the oral mucosa, ocular mucosa, ear mucosa, genital mucosa, pharyngeal mucosa, respiratory mucosa, bronchial mucosa, pulmonary mucosa, gastric mucosa, intestinal mucosa, or rectal mucosa. The present invention is a mucosal vaccine composition administered to at least one type of mucous membrane of a person or animal, the mucous membrane being selected from a group comprising the oral mucosa, ocular mucosa, ear mucosa, genital mucosa, pharyngeal mucosa, respiratory mucosa, bronchial mucosa, pulmonary mucosa, gastric mucosa, intestinal mucosa, and rectal mucosa. The mucosal vaccine composition is characterized by containing at least one type of antigen, and, as an immunostimulant agent, a lipopolysaccharide or a salt thereof derived from at least one type of a gram-negative bacterium selected from a group consisting of Serratia, Leclercia, Rahnella, Acidicaldus, Acidiphilium, Acidisphaera, Acidocella, Acidomonas, Asaia, Belnapia, Craurococcus, Gluconacetobacter, Gluconobacter, Kozakia, Leahibacter, Muricoccus, Neoasaia, Oleomonas, Paracraurococcus, Rhodopila, Roseococcus, Rubritepida, Saccharibacter, Stella, Swaminathania, Teichococcus, Zavarzinia, Pseudomonas, Achromobacter, Bacillus, Methanoculleus, Methanosarcina, Clostridium, Micrococcus, Flavobacterium, Pantoea, Acetobacter, Zymomonas, Xanthomonas, and Enterobacter. Moreover, the mucosal vaccine composition is characterized in that the mass ratio of the abovementioned immunostimulant agent and antigen (the total mass of the immunostimulant agent / the total mass of the antigen) is between 0.002 and 500.

Provided are methods for growing and shipping sprouts and microgreens in the same container, growing while in shipment using moisture provided in a water-absorbent layer, with optional added beneficials, and including methods for producing sprouts and microgreens for consumption, and for pharmaceutical / nutriceutical use, comprising growth of sprouts in retail-ready containers, the container comprising a moisture-retaining layer of agar media or the like providing water for growth and obviating the need for irrigation during sprout growth. In certain aspects, media is supplemented with beneficial organisms or additives such as probiotic microbes, vitamins (e.g., B12), cofactors, nutrients, and other items (e.g., phytochemicals, natural colors, and antioxidants) which promote the growth of the beneficial microbes on the product, and / or which become incorporated into the product. In certain aspects, added beneficial microorganisms are selected to compete antagonize human pathogens such as Listeria, Salmonella, enterohaemorrhagic E. coli, Yersinia, and / or spoilage organisms (e.g., Erwinia, Pseudomonas and Xanthomonas).

The invention provides a method for deeply treating landfill leachate. The method comprises the following steps: 1) after pretreating the landfill leachate, adopting an A / O process for treating; 2) after carrying out MBR film treatment and ultrafiltration treatmenton the landfill leachate treated in the step 1), allowing the landfill leachate to enter a same-position nitrification denitrificationfilter bed provided with a GY-3 type filler matrix for treatment; and 3) allowing the landfill leachate treated by the step 2) to enter a reverse osmosis system for treatment, and discharging treatedconcentrated water into a landfill or returning the treated concentrated water to the step 1); wherein the purified water is discharged up to the standard; and the GY-3 type filler matrix contains Pseudoxanthomonas, sphingomonas, Comamonadaceae and Hyphomicrobiaceae microorganisms. According to the treatment method provided by the invention, organic matters in the landfill leachate are degraded byapplying the GY-3 type filler matrix, and the purification and emission of the landfill leachate are realized; the treatment is efficient and rapid to start, the time is short, the treatment effect is stable, and the operation and running are simple and convenient.

The present invention presents a method for the aerobic production of xanthan by bacteria of the genus Xanthomonas on a solid or semi-solid substrate. In the exemplary embodiment of the method, a substrate is provided that has a total solids content of about 6.5% or higher. The substrate is sterilized and cooled. Bacteria of the genus Xanthomonas are inoculated into the substrate and incubated. After incubation, the bacteria are destroyed, the substrate is either diluted or washed, and the xanthan is isolated.

The invention discloses a method for detecting quarantine xanthomonas and a special primer thereof. The primer is composed of a first primer pair and a second primer pair. The first primer pair is composed of a primer XHpaAT-F and a primer XHpaAT-R. The second primer pair is composed of a primer XHpaAH-F and a primer XHpaAH-R. The nucleotide sequence of the primer XHpaAT-F, the nucleotide sequence of the primer XHpaAT-R, the nucleotide sequence of the primer XHpaAH-F and the nucleotide sequence of the primer XHpaAH-R are shown as the sequence 1, the sequence 2, the sequence 3 and the sequence 4 in the sequence list. Experiments show that by means of the method for detecting quarantine xanthomonas and the special primers thereof, whether a strain to be detected is quarantine xanthomonas or not can be identified simply, fast and effectively, accuracy is 100%, and the method has significant application to detection or identification of different kinds of quarantine pathogenic bacteria in xanthomonas.

This invention relates to bacteria of the genus Pseudoxanthomonas and in particular the strain deposited on Jul. 12, 2012 at the Pasteur Institute (CNCM, 25 rue du Docteur Roux, F-75724 PARIS Cedex 15, France) under No. CNCM 1-4657, capable of degrading MTBE and / or TBA.The invention also relates to a process for treatment of an effluent comprising MTBE and optionally TBA using such bacteria.

In order to provide a method for culturing an immunopotentiator-containing organism having the experience of being eaten, inexpensively without requiring usage of a component derived from an animal, Acetobacter, Gluconobacter, Xanthomonas, Zymomonas or Enterobacter which is an edible gram-negative bacterium having an immunopotentiation function is cultured using a culture solution composed mainly of wheat or bean curd refuse. Thereby, Acetobacter can be obtained inexpensively and safely, and also a low molecular weight lipopolysaccharide which is the immunopotentiator can be obtained inexpensively and safely. Furthermore, no impurity derived from animal components is mixed.