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Citrus trees with resistance to citrus canker

A technology of citrus canker and citrus, which is applied in the field of genetic improvement and recombinant DNA, and can solve problems such as difficult resistance

Inactive Publication Date: 2013-10-16
TWO BLADES FOUND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

An example of high levels of tolerance to ACC was reported in kumquat (Fortunella margarita); however, introgression of this resistance into widely bred citrus types such as sweet orange and grapefruit would be extremely difficult (Khalaf et al., ( 2008) Physiol. Mol. Plant Pathol. 71:240-250)

Method used

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  • Citrus trees with resistance to citrus canker
  • Citrus trees with resistance to citrus canker
  • Citrus trees with resistance to citrus canker

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Embodiment

[0127] Based on recent findings predicting activation of the UPT box by TAL effectors and the fact that at least one prominent TAL effector, PthA, is present in X. Trigger) gene fusion of several putative UPT boxes engineered super-promoters may be used to target PthA and other dominant AvrBs3 homologue proteins in X. Execute the gene and generate HR. Selection from Xanthomonas citri strain A W AvrGf1 (Rybak et al., (2009) Mol. Plant Pathol. 10:249-262) served as an executive gene due to its ability to stimulate HR in grapefruit once delivered into plant cells. A transient assay was developed in grapefruit (Citrus paradisi) to test constructs for this resistance regimen. This assay requires transformation of grapefruit leaves with Agrobacterium tumefaciens containing a T-DNA construct consisting of a Bs3 promoter construct fused to the executive gene avrGf1, followed by Xanthomonas citri The strains were co-inoculated on the same leaf area and responses were assessed. Tran...

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PUM

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Abstract

Methods and compositions for making citrus plants with enhanced resistance to Asiatic citrus canker (ACC) and other forms of citrus canker caused by Xanthonomas are provided. The methods involve transforming citrus plant cells with polynucleotide constructs comprising a promoter operably linked to nucleotide sequence that encodes a protein that is capable of triggering cell death in a citrus plant. The promoters of the invention are inducible by one or more Xanthomonas strains that cause citrus canker. Isolated nucleic acid molecules and expression cassettes comprising such polynucleotide constructs and promoters are further provided. Citrus plants with enhanced resistance to citrus canker are also provided.

Description

field of invention [0001] The present invention relates to the field of plant molecular biology, in particular the genetic improvement of plants by using methods involving recombinant DNA. Background technique [0002] Deployment of plant resistance has been the goal of many breeding programs to reduce losses due to plant diseases. With few exceptions, resistance to Asiatic citrus canker (ACC) is not commonly observed in citrus. An example of high levels of tolerance to ACC was reported in kumquat (Fortunella margarita); however, introgression of this resistance into widely bred citrus types such as sweet orange and grapefruit would be extremely difficult (Khalaf et al., ( 2008) Physiol. Mol. Plant Pathol. 71:240-250). [0003] ACC adversely affects citrus production worldwide (Gottwald et al. (2002) Phytopathol. 92:361-77). The causative agent of ACC is the following bacterial strain: Xanthomonas citri subsp. citri (X. citri), also known as X. campestris pv citri ), X.a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H5/08C07K14/415C12N15/82
CPCC07K14/415C12N15/8238C12N15/8263C12N15/8281C12N15/8239C12N15/8216
Inventor J·B·琼斯T·拉哈耶B·J·斯塔斯卡维茨
Owner TWO BLADES FOUND
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